Multi-omics profiling of hepatic macromolecules in laying hens with difference feed intake: Mechanistic insights into antioxidant capacity modulation

Currently, the cost of poultry feed accounts for more than 70 % of the total cost of poultry production. Therefore, it is crucial to find appropriate strategies to reduce feed cost and improve feed efficiency in livestock genetic improvement programs. The feed intake of poultry not only affects thei...

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Main Authors: Zhouyang Gao, Yuchen Jie, Yuhui Qin, Xianli Liu, Chuanwei Zheng, Guoming Ma, Yan Wu, Songchuan Fu, Xiangrui Meng, Guiyun Xu
Format: Article
Language:English
Published: Elsevier 2025-10-01
Series:Poultry Science
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Online Access:http://www.sciencedirect.com/science/article/pii/S003257912500817X
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author Zhouyang Gao
Yuchen Jie
Yuhui Qin
Xianli Liu
Chuanwei Zheng
Guoming Ma
Yan Wu
Songchuan Fu
Xiangrui Meng
Guiyun Xu
author_facet Zhouyang Gao
Yuchen Jie
Yuhui Qin
Xianli Liu
Chuanwei Zheng
Guoming Ma
Yan Wu
Songchuan Fu
Xiangrui Meng
Guiyun Xu
author_sort Zhouyang Gao
collection DOAJ
description Currently, the cost of poultry feed accounts for more than 70 % of the total cost of poultry production. Therefore, it is crucial to find appropriate strategies to reduce feed cost and improve feed efficiency in livestock genetic improvement programs. The feed intake of poultry not only affects their growth and development but also acts as a key factor that affects feed efficiency. Liver, the most important digestive and metabolic organ in the body, has a critical regulatory role in the feeding behavior of laying hens. However, to date, limited research has been conducted on the effect of the liver on the feed intake of laying hens. In the present study, we used RNA sequencing and liquid chromatography-mass spectrometry to analyze the differential genes and key metabolites in the livers of high-yield laying hens with low and high feed intake levels. The results indicated that the genes TRPM1, SLCO2A1, CFTR, GRIA1, and SLC4A5 as well as the metabolites l-threonine, l-ascorbate, and l-tyrosine were the key factors regulating feed intake in laying hens. Additionally, the measurement of antioxidant activity revealed that low-feed laying hens showed higher antioxidant enzyme activity than high-feed laying hens, which suggests that reducing feed intake does not negatively affect the health of laying hens.
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publisher Elsevier
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series Poultry Science
spelling doaj-art-3279e648c710412bb9178365e7b34f2c2025-08-20T03:41:04ZengElsevierPoultry Science0032-57912025-10-011041010557510.1016/j.psj.2025.105575Multi-omics profiling of hepatic macromolecules in laying hens with difference feed intake: Mechanistic insights into antioxidant capacity modulationZhouyang Gao0Yuchen Jie1Yuhui Qin2Xianli Liu3Chuanwei Zheng4Guoming Ma5Yan Wu6Songchuan Fu7Xiangrui Meng8Guiyun Xu9College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaCollege of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaCollege of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaBeinongda Technology Co., Ltd., Beijing 100083, ChinaBeinongda Technology Co., Ltd., Beijing 100083, ChinaBeinongda Technology Co., Ltd., Beijing 100083, ChinaBeinongda Technology Co., Ltd., Beijing 100083, ChinaInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaHinggan League Institute of Agricultural and Husbandry Sciences 137400, ChinaCollege of Animal Science and Technology, China Agricultural University, Beijing 100193, China; Corresponding author.Currently, the cost of poultry feed accounts for more than 70 % of the total cost of poultry production. Therefore, it is crucial to find appropriate strategies to reduce feed cost and improve feed efficiency in livestock genetic improvement programs. The feed intake of poultry not only affects their growth and development but also acts as a key factor that affects feed efficiency. Liver, the most important digestive and metabolic organ in the body, has a critical regulatory role in the feeding behavior of laying hens. However, to date, limited research has been conducted on the effect of the liver on the feed intake of laying hens. In the present study, we used RNA sequencing and liquid chromatography-mass spectrometry to analyze the differential genes and key metabolites in the livers of high-yield laying hens with low and high feed intake levels. The results indicated that the genes TRPM1, SLCO2A1, CFTR, GRIA1, and SLC4A5 as well as the metabolites l-threonine, l-ascorbate, and l-tyrosine were the key factors regulating feed intake in laying hens. Additionally, the measurement of antioxidant activity revealed that low-feed laying hens showed higher antioxidant enzyme activity than high-feed laying hens, which suggests that reducing feed intake does not negatively affect the health of laying hens.http://www.sciencedirect.com/science/article/pii/S003257912500817XLaying hensFeed intakeTranscriptomicsMetabolomicsAntioxidant capacity
spellingShingle Zhouyang Gao
Yuchen Jie
Yuhui Qin
Xianli Liu
Chuanwei Zheng
Guoming Ma
Yan Wu
Songchuan Fu
Xiangrui Meng
Guiyun Xu
Multi-omics profiling of hepatic macromolecules in laying hens with difference feed intake: Mechanistic insights into antioxidant capacity modulation
Poultry Science
Laying hens
Feed intake
Transcriptomics
Metabolomics
Antioxidant capacity
title Multi-omics profiling of hepatic macromolecules in laying hens with difference feed intake: Mechanistic insights into antioxidant capacity modulation
title_full Multi-omics profiling of hepatic macromolecules in laying hens with difference feed intake: Mechanistic insights into antioxidant capacity modulation
title_fullStr Multi-omics profiling of hepatic macromolecules in laying hens with difference feed intake: Mechanistic insights into antioxidant capacity modulation
title_full_unstemmed Multi-omics profiling of hepatic macromolecules in laying hens with difference feed intake: Mechanistic insights into antioxidant capacity modulation
title_short Multi-omics profiling of hepatic macromolecules in laying hens with difference feed intake: Mechanistic insights into antioxidant capacity modulation
title_sort multi omics profiling of hepatic macromolecules in laying hens with difference feed intake mechanistic insights into antioxidant capacity modulation
topic Laying hens
Feed intake
Transcriptomics
Metabolomics
Antioxidant capacity
url http://www.sciencedirect.com/science/article/pii/S003257912500817X
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