Fusion-Expressed CTB Improves Both Systemic and Mucosal T-Cell Responses Elicited by an Intranasal DNA Priming/Intramuscular Recombinant Vaccinia Boosting Regimen
Previous study showed that CTB (Cholera toxin subunit B) can be used as a genetic adjuvant to enhance the systemic immune responses. To further investigate whether it can also be used as a genetic adjuvant to improve mucosal immune responses, we constructed DNA and recombinant Tiantan vaccinia (rTTV...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2014/308732 |
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| author | Sugan Qiu Xiaonan Ren Yinyin Ben Yanqin Ren Jing Wang Xiaoyan Zhang Yanmin Wan Jianqing Xu |
| author_facet | Sugan Qiu Xiaonan Ren Yinyin Ben Yanqin Ren Jing Wang Xiaoyan Zhang Yanmin Wan Jianqing Xu |
| author_sort | Sugan Qiu |
| collection | DOAJ |
| description | Previous study showed that CTB (Cholera toxin subunit B) can be used as a genetic adjuvant to enhance the systemic immune responses. To further investigate whether it can also be used as a genetic adjuvant to improve mucosal immune responses, we constructed DNA and recombinant Tiantan vaccinia (rTTV) vaccines expressing OVA-CTB fusion antigen. Female C57BL/6 mice were immunized with an intranasal DNA priming/intramuscular rTTV boosting regimen. OVA specific T-cell responses were measured by IFN-γ ELISPOT and specific antibody responses were determined by ELISA. Compared to the nonadjuvant group (pSV-OVA intranasal priming/rTTV-OVA intramuscular boosting), pSV-OVA-CTB intranasal priming/rTTV-OVA-CTB intramuscular boosting group significantly improved the magnitudes of T-cell responses at spleen (1562±567 SFCs/106 splenocytes versus 330±182 SFCs/106 splenocytes, P<0.01), mesenteric LN (96±83 SFCs/106 lymphocytes versus 1±2 SFCs/106 lymphocytes, P<0.05), draining LNs of respiratory tract (109±60 SFCs/106 lymphocytes versus 2±2 SFCs/106 lymphocytes, P<0.01) and female genital tract (89±48 SFCs/106 lymphocytes versus 23±21 SFCs/106 lymphocytes, P<0.01). These results collectively demonstrated that fusion-expressed CTB could act as a potent adjuvant to improve both systemic and mucosal T-cell responses. |
| format | Article |
| id | doaj-art-32770b8070ad43e7b219ee96bc86e7cf |
| institution | OA Journals |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
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| series | Journal of Immunology Research |
| spelling | doaj-art-32770b8070ad43e7b219ee96bc86e7cf2025-08-20T02:24:04ZengWileyJournal of Immunology Research2314-88612314-71562014-01-01201410.1155/2014/308732308732Fusion-Expressed CTB Improves Both Systemic and Mucosal T-Cell Responses Elicited by an Intranasal DNA Priming/Intramuscular Recombinant Vaccinia Boosting RegimenSugan Qiu0Xiaonan Ren1Yinyin Ben2Yanqin Ren3Jing Wang4Xiaoyan Zhang5Yanmin Wan6Jianqing Xu7Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, ChinaShanghai Public Health Clinical Center, Fudan University, Shanghai 201508, ChinaShanghai Public Health Clinical Center, Fudan University, Shanghai 201508, ChinaShanghai Public Health Clinical Center, Fudan University, Shanghai 201508, ChinaShanghai Public Health Clinical Center, Fudan University, Shanghai 201508, ChinaShanghai Public Health Clinical Center, Fudan University, Shanghai 201508, ChinaShanghai Public Health Clinical Center, Fudan University, Shanghai 201508, ChinaShanghai Public Health Clinical Center, Fudan University, Shanghai 201508, ChinaPrevious study showed that CTB (Cholera toxin subunit B) can be used as a genetic adjuvant to enhance the systemic immune responses. To further investigate whether it can also be used as a genetic adjuvant to improve mucosal immune responses, we constructed DNA and recombinant Tiantan vaccinia (rTTV) vaccines expressing OVA-CTB fusion antigen. Female C57BL/6 mice were immunized with an intranasal DNA priming/intramuscular rTTV boosting regimen. OVA specific T-cell responses were measured by IFN-γ ELISPOT and specific antibody responses were determined by ELISA. Compared to the nonadjuvant group (pSV-OVA intranasal priming/rTTV-OVA intramuscular boosting), pSV-OVA-CTB intranasal priming/rTTV-OVA-CTB intramuscular boosting group significantly improved the magnitudes of T-cell responses at spleen (1562±567 SFCs/106 splenocytes versus 330±182 SFCs/106 splenocytes, P<0.01), mesenteric LN (96±83 SFCs/106 lymphocytes versus 1±2 SFCs/106 lymphocytes, P<0.05), draining LNs of respiratory tract (109±60 SFCs/106 lymphocytes versus 2±2 SFCs/106 lymphocytes, P<0.01) and female genital tract (89±48 SFCs/106 lymphocytes versus 23±21 SFCs/106 lymphocytes, P<0.01). These results collectively demonstrated that fusion-expressed CTB could act as a potent adjuvant to improve both systemic and mucosal T-cell responses.http://dx.doi.org/10.1155/2014/308732 |
| spellingShingle | Sugan Qiu Xiaonan Ren Yinyin Ben Yanqin Ren Jing Wang Xiaoyan Zhang Yanmin Wan Jianqing Xu Fusion-Expressed CTB Improves Both Systemic and Mucosal T-Cell Responses Elicited by an Intranasal DNA Priming/Intramuscular Recombinant Vaccinia Boosting Regimen Journal of Immunology Research |
| title | Fusion-Expressed CTB Improves Both Systemic and Mucosal T-Cell Responses Elicited by an Intranasal DNA Priming/Intramuscular Recombinant Vaccinia Boosting Regimen |
| title_full | Fusion-Expressed CTB Improves Both Systemic and Mucosal T-Cell Responses Elicited by an Intranasal DNA Priming/Intramuscular Recombinant Vaccinia Boosting Regimen |
| title_fullStr | Fusion-Expressed CTB Improves Both Systemic and Mucosal T-Cell Responses Elicited by an Intranasal DNA Priming/Intramuscular Recombinant Vaccinia Boosting Regimen |
| title_full_unstemmed | Fusion-Expressed CTB Improves Both Systemic and Mucosal T-Cell Responses Elicited by an Intranasal DNA Priming/Intramuscular Recombinant Vaccinia Boosting Regimen |
| title_short | Fusion-Expressed CTB Improves Both Systemic and Mucosal T-Cell Responses Elicited by an Intranasal DNA Priming/Intramuscular Recombinant Vaccinia Boosting Regimen |
| title_sort | fusion expressed ctb improves both systemic and mucosal t cell responses elicited by an intranasal dna priming intramuscular recombinant vaccinia boosting regimen |
| url | http://dx.doi.org/10.1155/2014/308732 |
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