Potential radiosensitive germline biomarkers in the testes of wild mice after the Fukushima accident
We investigated potential germline‐specific radiosensitive biomarkers in the testes of large Japanese field mice (Apodemus speciosus) exposed to low‐dose‐rate (LDR) radiation after the Fukushima accident. Fukushima wild mice testes were analysed via RNA‐sequencing to identify genes differentially ex...
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| Language: | English |
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Wiley
2025-02-01
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| Series: | FEBS Open Bio |
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| Online Access: | https://doi.org/10.1002/2211-5463.13927 |
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| author | Syun Tokita Ryo Nakayama Yohei Fujishima Valerie Swee Ting Goh Donovan Anderson Ippei Uemura Hikari Ikema Jin Shibata Yoh Kinoshita Yoshinaka Shimizu Hisashi Shinoda Jun Goto Maria Grazia Palmerini Abdulla Mohamed Hatha Takashi Satoh Akifumi Nakata Manabu Fukumoto Tomisato Miura Hideaki Yamashiro |
| author_facet | Syun Tokita Ryo Nakayama Yohei Fujishima Valerie Swee Ting Goh Donovan Anderson Ippei Uemura Hikari Ikema Jin Shibata Yoh Kinoshita Yoshinaka Shimizu Hisashi Shinoda Jun Goto Maria Grazia Palmerini Abdulla Mohamed Hatha Takashi Satoh Akifumi Nakata Manabu Fukumoto Tomisato Miura Hideaki Yamashiro |
| author_sort | Syun Tokita |
| collection | DOAJ |
| description | We investigated potential germline‐specific radiosensitive biomarkers in the testes of large Japanese field mice (Apodemus speciosus) exposed to low‐dose‐rate (LDR) radiation after the Fukushima accident. Fukushima wild mice testes were analysed via RNA‐sequencing to identify genes differentially expressed in the breeding and non‐breeding seasons when compared to controls. Results revealed significant changes during the breeding season, with Lsp1 showing a considerable upregulation, while Ptprk and Tspear exhibited significant reductions. Conversely, in the non‐breeding season, Fmo2 and Fmo2 (highly similar) were significantly upregulated in radiation‐exposed Fukushima mice. qPCR analysis results were consistent with transcriptome sequencing, detecting Lsp1 and Ptprk regulation in the testes of Fukushima mice. While differences in gene expression were observed, these do not imply any causal association between the identified biomarkers and chronic LDR exposure, as other factors such as the environment and developmental age may contribute. This study provides valuable insights into the reproductive biology is affected by environmental radiation and highlights the value of assessing the effects of chronic LDR radiation exposure on testicular health in wild mice. |
| format | Article |
| id | doaj-art-3276505dddc5441daf0b1e327bd6fa38 |
| institution | Kabale University |
| issn | 2211-5463 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj-art-3276505dddc5441daf0b1e327bd6fa382025-02-03T10:59:30ZengWileyFEBS Open Bio2211-54632025-02-0115229631010.1002/2211-5463.13927Potential radiosensitive germline biomarkers in the testes of wild mice after the Fukushima accidentSyun Tokita0Ryo Nakayama1Yohei Fujishima2Valerie Swee Ting Goh3Donovan Anderson4Ippei Uemura5Hikari Ikema6Jin Shibata7Yoh Kinoshita8Yoshinaka Shimizu9Hisashi Shinoda10Jun Goto11Maria Grazia Palmerini12Abdulla Mohamed Hatha13Takashi Satoh14Akifumi Nakata15Manabu Fukumoto16Tomisato Miura17Hideaki Yamashiro18Graduate School of Science and Technology Niigata University JapanDepartment of Risk Analysis and Biodosimetry, Institute of Radiation Emergency Medicine Hirosaki University Aomori JapanDepartment of Risk Analysis and Biodosimetry, Institute of Radiation Emergency Medicine Hirosaki University Aomori JapanDepartment of Radiobiology, Singapore Nuclear Research and Safety Initiative National University of Singapore SingaporeDepartment of Risk Analysis and Biodosimetry, Institute of Radiation Emergency Medicine Hirosaki University Aomori JapanFaculty of Pharmaceutical Sciences Hokkaido University of Science Sapporo JapanGraduate School of Science and Technology Niigata University JapanGraduate School of Science and Technology Niigata University JapanGraduate School of Science and Technology Niigata University JapanGraduate School of Dentistry Tohoku University Sendai JapanGraduate School of Dentistry Tohoku University Sendai JapanInstitute for Research Administration Niigata University JapanDepartment of Life, Health and Environmental Sciences University of L'Aquila ItalyDepartment of Marine Biology, Microbiology, Biochemistry Cochin University of Science and Technology IndiaFaculty of Pharmaceutical Sciences Hokkaido University of Science Sapporo JapanFaculty of Pharmaceutical Sciences Hokkaido University of Science Sapporo JapanRIKEN Centre for Advanced Intelligence Project Pathology Informatics Team Tokyo JapanDepartment of Risk Analysis and Biodosimetry, Institute of Radiation Emergency Medicine Hirosaki University Aomori JapanGraduate School of Science and Technology Niigata University JapanWe investigated potential germline‐specific radiosensitive biomarkers in the testes of large Japanese field mice (Apodemus speciosus) exposed to low‐dose‐rate (LDR) radiation after the Fukushima accident. Fukushima wild mice testes were analysed via RNA‐sequencing to identify genes differentially expressed in the breeding and non‐breeding seasons when compared to controls. Results revealed significant changes during the breeding season, with Lsp1 showing a considerable upregulation, while Ptprk and Tspear exhibited significant reductions. Conversely, in the non‐breeding season, Fmo2 and Fmo2 (highly similar) were significantly upregulated in radiation‐exposed Fukushima mice. qPCR analysis results were consistent with transcriptome sequencing, detecting Lsp1 and Ptprk regulation in the testes of Fukushima mice. While differences in gene expression were observed, these do not imply any causal association between the identified biomarkers and chronic LDR exposure, as other factors such as the environment and developmental age may contribute. This study provides valuable insights into the reproductive biology is affected by environmental radiation and highlights the value of assessing the effects of chronic LDR radiation exposure on testicular health in wild mice.https://doi.org/10.1002/2211-5463.13927Apodemus speciosusFukushima accidentlow‐dose‐rate radiationradiosensitive biomarkers |
| spellingShingle | Syun Tokita Ryo Nakayama Yohei Fujishima Valerie Swee Ting Goh Donovan Anderson Ippei Uemura Hikari Ikema Jin Shibata Yoh Kinoshita Yoshinaka Shimizu Hisashi Shinoda Jun Goto Maria Grazia Palmerini Abdulla Mohamed Hatha Takashi Satoh Akifumi Nakata Manabu Fukumoto Tomisato Miura Hideaki Yamashiro Potential radiosensitive germline biomarkers in the testes of wild mice after the Fukushima accident FEBS Open Bio Apodemus speciosus Fukushima accident low‐dose‐rate radiation radiosensitive biomarkers |
| title | Potential radiosensitive germline biomarkers in the testes of wild mice after the Fukushima accident |
| title_full | Potential radiosensitive germline biomarkers in the testes of wild mice after the Fukushima accident |
| title_fullStr | Potential radiosensitive germline biomarkers in the testes of wild mice after the Fukushima accident |
| title_full_unstemmed | Potential radiosensitive germline biomarkers in the testes of wild mice after the Fukushima accident |
| title_short | Potential radiosensitive germline biomarkers in the testes of wild mice after the Fukushima accident |
| title_sort | potential radiosensitive germline biomarkers in the testes of wild mice after the fukushima accident |
| topic | Apodemus speciosus Fukushima accident low‐dose‐rate radiation radiosensitive biomarkers |
| url | https://doi.org/10.1002/2211-5463.13927 |
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