Recent advances in biosynthesis of non-canonical amino acids and their potentials in strain engineering
Non-canonical amino acids (ncAAs), as derivatives of canonical amino acids (cAAs), when specifically incorporated into proteins, could enrich protein functions. Currently, ncAAs mostly depend on chemical synthesis; however, biosynthesis of ncAAs could solve the problems of low transmembrane transpor...
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| Language: | English |
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Elsevier
2025-03-01
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| Series: | Results in Engineering |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S259012302401884X |
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| author | Zhen Hou Junkai Tuo Xiaoyan Ma Yi-Xin Huo |
| author_facet | Zhen Hou Junkai Tuo Xiaoyan Ma Yi-Xin Huo |
| author_sort | Zhen Hou |
| collection | DOAJ |
| description | Non-canonical amino acids (ncAAs), as derivatives of canonical amino acids (cAAs), when specifically incorporated into proteins, could enrich protein functions. Currently, ncAAs mostly depend on chemical synthesis; however, biosynthesis of ncAAs could solve the problems of low transmembrane transport efficiency and potential toxicity of exogenously added ncAAs. Moreover, the biosynthesis of ncAAs is eco-friendly and economical. In this article, we firstly reviewed recent advances in the biosynthesis strategies of ncAAs, including hijacking the biosynthesis pathways of cAAs, deriving ncAAs from cAAs, and de novo biosynthesis of ncAAs, from the advantages, shortcomings, and potentials of these three strategies. Secondly, we discussed the applications of ncAAs in bacterial-based therapeutics, whole-cell biocatalysts, and biosafety. Finally, we provide guidance for the improvement of ncAAs biosynthesis and put forward perspectives on applying ncAAs in strain engineering. This work will promote the in situ synthesis of ncAAs modified proteins and unleash the potentials of ncAAs in strain engineering. |
| format | Article |
| id | doaj-art-326aaaa57f0340fa83f13e3f56d7db1b |
| institution | DOAJ |
| issn | 2590-1230 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Results in Engineering |
| spelling | doaj-art-326aaaa57f0340fa83f13e3f56d7db1b2025-08-20T02:52:08ZengElsevierResults in Engineering2590-12302025-03-012510364110.1016/j.rineng.2024.103641Recent advances in biosynthesis of non-canonical amino acids and their potentials in strain engineeringZhen Hou0Junkai Tuo1Xiaoyan Ma2Yi-Xin Huo3Key Laboratory of Molecular Medicine and Biotherapy, School of Life Science, Beijing Institute of Technology, Beijing 100081, PR ChinaKey Laboratory of Molecular Medicine and Biotherapy, School of Life Science, Beijing Institute of Technology, Beijing 100081, PR ChinaKey Laboratory of Molecular Medicine and Biotherapy, School of Life Science, Beijing Institute of Technology, Beijing 100081, PR China; Tangshan Research Institute, Beijing Institute of Technology, Hebei 063000, PR China; Center for Future Foods, Muyuan Laboratory, 110 ShangdingRoad, Zhengzhou, Henan Province 450016, PR China; Corresponding author at: Key Laboratory of Molecular Medicine and Biotherapy, School of Life Science, Beijing Institute of Technology, Beijing 100081, PR China.Department of gastroenterology, Aerospace Center Hospital, School of Life Science, Beijing Institute of Technology, Beijing 100081, PR China; Key Laboratory of Molecular Medicine and Biotherapy, School of Life Science, Beijing Institute of Technology, Beijing 100081, PR China; Tangshan Research Institute, Beijing Institute of Technology, Hebei 063000, PR China; Center for Future Foods, Muyuan Laboratory, 110 ShangdingRoad, Zhengzhou, Henan Province 450016, PR ChinaNon-canonical amino acids (ncAAs), as derivatives of canonical amino acids (cAAs), when specifically incorporated into proteins, could enrich protein functions. Currently, ncAAs mostly depend on chemical synthesis; however, biosynthesis of ncAAs could solve the problems of low transmembrane transport efficiency and potential toxicity of exogenously added ncAAs. Moreover, the biosynthesis of ncAAs is eco-friendly and economical. In this article, we firstly reviewed recent advances in the biosynthesis strategies of ncAAs, including hijacking the biosynthesis pathways of cAAs, deriving ncAAs from cAAs, and de novo biosynthesis of ncAAs, from the advantages, shortcomings, and potentials of these three strategies. Secondly, we discussed the applications of ncAAs in bacterial-based therapeutics, whole-cell biocatalysts, and biosafety. Finally, we provide guidance for the improvement of ncAAs biosynthesis and put forward perspectives on applying ncAAs in strain engineering. This work will promote the in situ synthesis of ncAAs modified proteins and unleash the potentials of ncAAs in strain engineering.http://www.sciencedirect.com/science/article/pii/S259012302401884XBiosynthesisEnzyme engineeringGenetic code expansionUnnatural amino acidsEngineered bacterial therapeutics |
| spellingShingle | Zhen Hou Junkai Tuo Xiaoyan Ma Yi-Xin Huo Recent advances in biosynthesis of non-canonical amino acids and their potentials in strain engineering Results in Engineering Biosynthesis Enzyme engineering Genetic code expansion Unnatural amino acids Engineered bacterial therapeutics |
| title | Recent advances in biosynthesis of non-canonical amino acids and their potentials in strain engineering |
| title_full | Recent advances in biosynthesis of non-canonical amino acids and their potentials in strain engineering |
| title_fullStr | Recent advances in biosynthesis of non-canonical amino acids and their potentials in strain engineering |
| title_full_unstemmed | Recent advances in biosynthesis of non-canonical amino acids and their potentials in strain engineering |
| title_short | Recent advances in biosynthesis of non-canonical amino acids and their potentials in strain engineering |
| title_sort | recent advances in biosynthesis of non canonical amino acids and their potentials in strain engineering |
| topic | Biosynthesis Enzyme engineering Genetic code expansion Unnatural amino acids Engineered bacterial therapeutics |
| url | http://www.sciencedirect.com/science/article/pii/S259012302401884X |
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