Administration Strategy-Dependent Mechanisms and Effects of Human Adipose Tissue Stem Cell Extracellular Vesicles in Mouse Allergic Rhinitis Treatment
We previously found that intravenous injection of extracellular vesicles (EVs) from human adipose tissue–derived stem cells (hADSC) could ameliorate allergic rhinitis (AR) in mice through immunomodulatory effects. In clinical trials, nasal delivery has been an attractive treatment for AR. We sought...
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SAGE Publishing
2025-04-01
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| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.1177/09636897251325673 |
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| author | Wenhan Yang Zhiyu Pan Jiacheng Zhang Lian Wang Ju Lai Kai Fan Jingjing Zhu Qian Liu Yalei Dai Jieyu Zhou Shuhui Wu Zhengliang Gao Shaoqing Yu |
| author_facet | Wenhan Yang Zhiyu Pan Jiacheng Zhang Lian Wang Ju Lai Kai Fan Jingjing Zhu Qian Liu Yalei Dai Jieyu Zhou Shuhui Wu Zhengliang Gao Shaoqing Yu |
| author_sort | Wenhan Yang |
| collection | DOAJ |
| description | We previously found that intravenous injection of extracellular vesicles (EVs) from human adipose tissue–derived stem cells (hADSC) could ameliorate allergic rhinitis (AR) in mice through immunomodulatory effects. In clinical trials, nasal delivery has been an attractive treatment for AR. We sought to determine whether there are differences in the therapeutic effects between caudal injection and their combination. We treated AR mice with ADSC-EVs via caudal vein, nasal cavity, or both. After treatment, the mice were re-sensitized and the indices of behavior, nasal mucosa morphology, and cytokine secretion of the mice under different modes of administration were calculated. The resultes show that tail vein, nasal, and combined administration could effectively relieve the inflammatory infiltration of the nasal mucosa of mice, reduce the secretion of IgE, IL-4, and other inflammatory factors, and alleviate the Th1/Th2 imbalance. Injection and nasal delivery, as well as their combination, effectively alleviated the symptoms of rhinitis in mice. Nasal administration has a better therapeutic effect when the inflammatory response is mild. It could be speculated that ADSC-EVs have excellent properties in the treatment of AR, and modes of administration can be selected for different stages of treatment in clinical therapy. |
| format | Article |
| id | doaj-art-325ab8a652394272a4850a35ef18ebd5 |
| institution | DOAJ |
| issn | 1555-3892 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj-art-325ab8a652394272a4850a35ef18ebd52025-08-20T03:16:57ZengSAGE PublishingCell Transplantation1555-38922025-04-013410.1177/09636897251325673Administration Strategy-Dependent Mechanisms and Effects of Human Adipose Tissue Stem Cell Extracellular Vesicles in Mouse Allergic Rhinitis TreatmentWenhan Yang0Zhiyu Pan1Jiacheng Zhang2Lian Wang3Ju Lai4Kai Fan5Jingjing Zhu6Qian Liu7Yalei Dai8Jieyu Zhou9Shuhui Wu10Zhengliang Gao11Shaoqing Yu12Shanghai Engineering Research Center of Organ Repair, School of Medicine, Shanghai University, Shanghai, ChinaDepartment of Otolaryngology-Head and Neck Surgery, Tongji Hospital, School of Medicine, Tongji University, Shanghai, ChinaDepartment of Otolaryngology-Head and Neck Surgery, Tongji Hospital, School of Medicine, Tongji University, Shanghai, ChinaDepartment of Otolaryngology-Head and Neck Surgery, Tongji Hospital, School of Medicine, Tongji University, Shanghai, ChinaDepartment of Anesthesiology, Shanghai Gongli Hospital, Naval Military Medical University, Shanghai, ChinaDepartment of Otolaryngology-Head and Neck Surgery, Tongji Hospital, School of Medicine, Tongji University, Shanghai, ChinaDepartment of Otorhinolaryngology, Shanghai Baoshan Hospital of Integrated Traditional Chinese and Western Medicine, Baoshan Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaDepartment of Urology, Tianjin First Central Hospital, Tianjin, ChinaDepartment of Otolaryngology-Head and Neck Surgery, Tongji Hospital, School of Medicine, Tongji University, Shanghai, ChinaDepartment of Otorhinolaryngology-Head and Neck Surgery, Shanghai Ninth People’s Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, ChinaDepartment of Otorhinolaryngology, Shanghai Baoshan Hospital of Integrated Traditional Chinese and Western Medicine, Baoshan Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaChina-Japan Friendship Medical Research Institute, Shanghai University, Shanghai, ChinaDepartment of Allergy, School of Medicine, Tongji Hospital, Tongji University, Shanghai, ChinaWe previously found that intravenous injection of extracellular vesicles (EVs) from human adipose tissue–derived stem cells (hADSC) could ameliorate allergic rhinitis (AR) in mice through immunomodulatory effects. In clinical trials, nasal delivery has been an attractive treatment for AR. We sought to determine whether there are differences in the therapeutic effects between caudal injection and their combination. We treated AR mice with ADSC-EVs via caudal vein, nasal cavity, or both. After treatment, the mice were re-sensitized and the indices of behavior, nasal mucosa morphology, and cytokine secretion of the mice under different modes of administration were calculated. The resultes show that tail vein, nasal, and combined administration could effectively relieve the inflammatory infiltration of the nasal mucosa of mice, reduce the secretion of IgE, IL-4, and other inflammatory factors, and alleviate the Th1/Th2 imbalance. Injection and nasal delivery, as well as their combination, effectively alleviated the symptoms of rhinitis in mice. Nasal administration has a better therapeutic effect when the inflammatory response is mild. It could be speculated that ADSC-EVs have excellent properties in the treatment of AR, and modes of administration can be selected for different stages of treatment in clinical therapy.https://doi.org/10.1177/09636897251325673 |
| spellingShingle | Wenhan Yang Zhiyu Pan Jiacheng Zhang Lian Wang Ju Lai Kai Fan Jingjing Zhu Qian Liu Yalei Dai Jieyu Zhou Shuhui Wu Zhengliang Gao Shaoqing Yu Administration Strategy-Dependent Mechanisms and Effects of Human Adipose Tissue Stem Cell Extracellular Vesicles in Mouse Allergic Rhinitis Treatment Cell Transplantation |
| title | Administration Strategy-Dependent Mechanisms and Effects of Human Adipose Tissue Stem Cell Extracellular Vesicles in Mouse Allergic Rhinitis Treatment |
| title_full | Administration Strategy-Dependent Mechanisms and Effects of Human Adipose Tissue Stem Cell Extracellular Vesicles in Mouse Allergic Rhinitis Treatment |
| title_fullStr | Administration Strategy-Dependent Mechanisms and Effects of Human Adipose Tissue Stem Cell Extracellular Vesicles in Mouse Allergic Rhinitis Treatment |
| title_full_unstemmed | Administration Strategy-Dependent Mechanisms and Effects of Human Adipose Tissue Stem Cell Extracellular Vesicles in Mouse Allergic Rhinitis Treatment |
| title_short | Administration Strategy-Dependent Mechanisms and Effects of Human Adipose Tissue Stem Cell Extracellular Vesicles in Mouse Allergic Rhinitis Treatment |
| title_sort | administration strategy dependent mechanisms and effects of human adipose tissue stem cell extracellular vesicles in mouse allergic rhinitis treatment |
| url | https://doi.org/10.1177/09636897251325673 |
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