Structural insights and clinical advances in small-molecule inhibitors targeting TGF-β receptor I

The dysregulation of the transforming growth factor β (TGF-β) signaling pathway plays a critical role in the onset and progression of several diseases, including cancer. Notably, TGF-β has emerged as a significant barrier to effective outcomes in cancer immunotherapies, particularly those using immu...

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Main Authors: Carlota Leonardo-Sousa, Rodrigo Barriga, Helena F. Florindo, Rita C. Acúrcio, Rita C. Guedes
Format: Article
Language:English
Published: Elsevier 2025-03-01
Series:Molecular Therapy: Oncology
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Online Access:http://www.sciencedirect.com/science/article/pii/S2950329925000141
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author Carlota Leonardo-Sousa
Rodrigo Barriga
Helena F. Florindo
Rita C. Acúrcio
Rita C. Guedes
author_facet Carlota Leonardo-Sousa
Rodrigo Barriga
Helena F. Florindo
Rita C. Acúrcio
Rita C. Guedes
author_sort Carlota Leonardo-Sousa
collection DOAJ
description The dysregulation of the transforming growth factor β (TGF-β) signaling pathway plays a critical role in the onset and progression of several diseases, including cancer. Notably, TGF-β has emerged as a significant barrier to effective outcomes in cancer immunotherapies, particularly those using immune checkpoint inhibitors. In response to this challenge, small-molecule inhibitors targeting the TGF-β receptor I (TGF-βRI) have garnered attention as promising candidates for modulating the TGF-β signaling pathway. This comprehensive review focuses on the development of small-molecule inhibitors targeting TGF-βRI. We provide a detailed analysis of the structural biology of TGF-βRI, highlighting key binding interactions and structural insights derived from high-resolution X-ray crystal structures. Additionally, we review the current landscape of TGF-βRI inhibitors in clinical trials, including eight promising inhibitors, and discuss their mechanisms of action, selectivity, and therapeutic potential. Our investigation extends to the patent literature, summarizing over 2 decades of innovation from leading pharmaceutical companies, spanning January 2000–May 2024. This consolidated structural and biochemical knowledge aims to facilitate the design of next-generation TGF-βRI inhibitors, addressing unmet clinical needs in oncology and fibrosis treatment. The synergistic potential of combining TGF-βRI and immune checkpoint inhibitors is also explored, offering promising avenues for enhancing cancer immunotherapy efficacy.
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series Molecular Therapy: Oncology
spelling doaj-art-32586f4f5bbb40b28290bebf185f4f5a2025-08-20T02:02:01ZengElsevierMolecular Therapy: Oncology2950-32992025-03-0133120094510.1016/j.omton.2025.200945Structural insights and clinical advances in small-molecule inhibitors targeting TGF-β receptor ICarlota Leonardo-Sousa0Rodrigo Barriga1Helena F. Florindo2Rita C. Acúrcio3Rita C. Guedes4Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, 1649-003 Lisboa, PortugalResearch Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, 1649-003 Lisboa, PortugalResearch Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, 1649-003 Lisboa, Portugal; Corresponding author: Helena F. Florindo, Ph.D., Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, 1649-003 Lisboa, Portugal.Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, 1649-003 Lisboa, Portugal; Corresponding author: Rita C. Acúrcio Ph.D., Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, 1649-003 Lisboa, Portugal.Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, 1649-003 Lisboa, Portugal; Corresponding author: Rita C. Guedes, Ph.D., Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, 1649-003 Lisboa, Portugal.The dysregulation of the transforming growth factor β (TGF-β) signaling pathway plays a critical role in the onset and progression of several diseases, including cancer. Notably, TGF-β has emerged as a significant barrier to effective outcomes in cancer immunotherapies, particularly those using immune checkpoint inhibitors. In response to this challenge, small-molecule inhibitors targeting the TGF-β receptor I (TGF-βRI) have garnered attention as promising candidates for modulating the TGF-β signaling pathway. This comprehensive review focuses on the development of small-molecule inhibitors targeting TGF-βRI. We provide a detailed analysis of the structural biology of TGF-βRI, highlighting key binding interactions and structural insights derived from high-resolution X-ray crystal structures. Additionally, we review the current landscape of TGF-βRI inhibitors in clinical trials, including eight promising inhibitors, and discuss their mechanisms of action, selectivity, and therapeutic potential. Our investigation extends to the patent literature, summarizing over 2 decades of innovation from leading pharmaceutical companies, spanning January 2000–May 2024. This consolidated structural and biochemical knowledge aims to facilitate the design of next-generation TGF-βRI inhibitors, addressing unmet clinical needs in oncology and fibrosis treatment. The synergistic potential of combining TGF-βRI and immune checkpoint inhibitors is also explored, offering promising avenues for enhancing cancer immunotherapy efficacy.http://www.sciencedirect.com/science/article/pii/S2950329925000141MT: Regular Issuetransforming growth factor βTGF-βTGF-β receptor ITGF-βRITGF-βRI inhibitors
spellingShingle Carlota Leonardo-Sousa
Rodrigo Barriga
Helena F. Florindo
Rita C. Acúrcio
Rita C. Guedes
Structural insights and clinical advances in small-molecule inhibitors targeting TGF-β receptor I
Molecular Therapy: Oncology
MT: Regular Issue
transforming growth factor β
TGF-β
TGF-β receptor I
TGF-βRI
TGF-βRI inhibitors
title Structural insights and clinical advances in small-molecule inhibitors targeting TGF-β receptor I
title_full Structural insights and clinical advances in small-molecule inhibitors targeting TGF-β receptor I
title_fullStr Structural insights and clinical advances in small-molecule inhibitors targeting TGF-β receptor I
title_full_unstemmed Structural insights and clinical advances in small-molecule inhibitors targeting TGF-β receptor I
title_short Structural insights and clinical advances in small-molecule inhibitors targeting TGF-β receptor I
title_sort structural insights and clinical advances in small molecule inhibitors targeting tgf β receptor i
topic MT: Regular Issue
transforming growth factor β
TGF-β
TGF-β receptor I
TGF-βRI
TGF-βRI inhibitors
url http://www.sciencedirect.com/science/article/pii/S2950329925000141
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AT helenafflorindo structuralinsightsandclinicaladvancesinsmallmoleculeinhibitorstargetingtgfbreceptori
AT ritacacurcio structuralinsightsandclinicaladvancesinsmallmoleculeinhibitorstargetingtgfbreceptori
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