Potential Antimalarial Compounds from Spectroscopically Identified Compounds of Aqueous Stem-bark Extract of Magnifera indica: An In silico Approach

Ethnobotanical studies and phytochemical screening are invaluable approaches in the search for novel drug substances needed to confront the growing challenges faced by drugs used in the treatment of many infectious diseases malaria inclusive. This study employed spectroscopic techniques to identify...

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Main Authors: Abdulhafiz Lamiya, Saihana Muhammad, Adamu Jibril Alhassan, Maryam AbdulKadir Dangambo, Hauwa Ahmed Zailani
Format: Article
Language:English
Published: Biotechnology Society of Nepal 2025-07-01
Series:Nepal Journal of Biotechnology
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Online Access:https://nepjb.com/index.php/NJB/article/view/383
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author Abdulhafiz Lamiya
Saihana Muhammad
Adamu Jibril Alhassan
Maryam AbdulKadir Dangambo
Hauwa Ahmed Zailani
author_facet Abdulhafiz Lamiya
Saihana Muhammad
Adamu Jibril Alhassan
Maryam AbdulKadir Dangambo
Hauwa Ahmed Zailani
author_sort Abdulhafiz Lamiya
collection DOAJ
description Ethnobotanical studies and phytochemical screening are invaluable approaches in the search for novel drug substances needed to confront the growing challenges faced by drugs used in the treatment of many infectious diseases malaria inclusive. This study employed spectroscopic techniques to identify major phytochemicals present in aqueous extract of Magnifera indica stem bark and in silico technique to screen the identified phytochemicals for the identification of potential Plasmodium falciparum protein inhibitors. Spectroscopic assay of the extract revealed the presence of fifteen major compounds. Molecular docking analysis of the identified compounds against some Plasmodium falciparum targets revealed that oleic acid was the top hit compound for plasmepsin II with binding energy of -5.8Kcal/mol., 9,12-Octadecadienoic acid [Z,Z]  was the top hit for histo-aspartic protease with binding energy of -6.1Kcal/mol., and Phthalic acid dibutyl ester was the to hit compound for both falcipain-2 and P. falciparum enoyl acyl carrier protein reductase with binding energy of  -5.6 and -4.9Kcal/mol respectively. ADMET analysis of these top hit compounds revealed favorable pharmacokinetics and toxicity. All the compounds poses drug like properties, high GI absorption, non-substrate to permeability-glycoprotein and safe for the major vital organs except Phthalic acid dibutyl ester which shows slight activity in nephrotoxicity. Thus, there is need for further experimental validation and optimization of the top hit compounds to improve both efficacy and toxicity. 
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institution Kabale University
issn 2091-1130
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publishDate 2025-07-01
publisher Biotechnology Society of Nepal
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spelling doaj-art-3255ed778abe42f2a347b70337b4ee822025-08-20T03:36:31ZengBiotechnology Society of NepalNepal Journal of Biotechnology2091-11302467-93132025-07-0113110.54796/njb.v13i1.383Potential Antimalarial Compounds from Spectroscopically Identified Compounds of Aqueous Stem-bark Extract of Magnifera indica: An In silico ApproachAbdulhafiz Lamiya0https://orcid.org/0000-0002-5401-3937Saihana Muhammad1https://orcid.org/0009-0008-7528-0533Adamu Jibril Alhassan2https://orcid.org/0000-0002-4626-3370Maryam AbdulKadir Dangambo3https://orcid.org/0000-0001-9948-6677Hauwa Ahmed Zailani4https://orcid.org/0000-0003-2930-430XDepartment of Science Laboratory Technology, Modibbo Adama University Yola, NigeriaDepartment of Biochemistry, Aliko Dangote University of Science and Technology Wudil Kano State, NigeriaDepartment of Biochemistry, Bayero University Kano, NigeriaDepartment of Biochemistry, Bayero University Kano, NigeriaDepartemnt of Biochemistry, Modibbo Adama University Yola, Adamawa state, Nigeria Ethnobotanical studies and phytochemical screening are invaluable approaches in the search for novel drug substances needed to confront the growing challenges faced by drugs used in the treatment of many infectious diseases malaria inclusive. This study employed spectroscopic techniques to identify major phytochemicals present in aqueous extract of Magnifera indica stem bark and in silico technique to screen the identified phytochemicals for the identification of potential Plasmodium falciparum protein inhibitors. Spectroscopic assay of the extract revealed the presence of fifteen major compounds. Molecular docking analysis of the identified compounds against some Plasmodium falciparum targets revealed that oleic acid was the top hit compound for plasmepsin II with binding energy of -5.8Kcal/mol., 9,12-Octadecadienoic acid [Z,Z]  was the top hit for histo-aspartic protease with binding energy of -6.1Kcal/mol., and Phthalic acid dibutyl ester was the to hit compound for both falcipain-2 and P. falciparum enoyl acyl carrier protein reductase with binding energy of  -5.6 and -4.9Kcal/mol respectively. ADMET analysis of these top hit compounds revealed favorable pharmacokinetics and toxicity. All the compounds poses drug like properties, high GI absorption, non-substrate to permeability-glycoprotein and safe for the major vital organs except Phthalic acid dibutyl ester which shows slight activity in nephrotoxicity. Thus, there is need for further experimental validation and optimization of the top hit compounds to improve both efficacy and toxicity.  https://nepjb.com/index.php/NJB/article/view/383antimalarial phytocompoundsdrug discoveryM. indicapharmacokinetics
spellingShingle Abdulhafiz Lamiya
Saihana Muhammad
Adamu Jibril Alhassan
Maryam AbdulKadir Dangambo
Hauwa Ahmed Zailani
Potential Antimalarial Compounds from Spectroscopically Identified Compounds of Aqueous Stem-bark Extract of Magnifera indica: An In silico Approach
Nepal Journal of Biotechnology
antimalarial phytocompounds
drug discovery
M. indica
pharmacokinetics
title Potential Antimalarial Compounds from Spectroscopically Identified Compounds of Aqueous Stem-bark Extract of Magnifera indica: An In silico Approach
title_full Potential Antimalarial Compounds from Spectroscopically Identified Compounds of Aqueous Stem-bark Extract of Magnifera indica: An In silico Approach
title_fullStr Potential Antimalarial Compounds from Spectroscopically Identified Compounds of Aqueous Stem-bark Extract of Magnifera indica: An In silico Approach
title_full_unstemmed Potential Antimalarial Compounds from Spectroscopically Identified Compounds of Aqueous Stem-bark Extract of Magnifera indica: An In silico Approach
title_short Potential Antimalarial Compounds from Spectroscopically Identified Compounds of Aqueous Stem-bark Extract of Magnifera indica: An In silico Approach
title_sort potential antimalarial compounds from spectroscopically identified compounds of aqueous stem bark extract of magnifera indica an in silico approach
topic antimalarial phytocompounds
drug discovery
M. indica
pharmacokinetics
url https://nepjb.com/index.php/NJB/article/view/383
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