Responses of largemouth bass (Micropterus salmoides) to chronic alkalinity stress: Survival, growth performance, physiology, and muscle quality

Saline-alkaline water fishery has emerged as a highly promising aquaculture mode in China. To identify potential species suitable for saline-alkaline water aquaculture, this study investigated the effects of different alkalinity stresses (0, 7, 14, 21, 28 mmol/L) on the survival, growth performance,...

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Main Authors: Xi Shi, Shijie Yuan, Wei Peng, Fengqing Chen, Xiangwei Liu, Xueke Ma, Xiao Ma, Ruwei Xu, Khor Waiho, Xianghui Kong, Cuiyun Lu, Xuejun Li
Format: Article
Language:English
Published: Elsevier 2025-07-01
Series:Aquaculture Reports
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Online Access:http://www.sciencedirect.com/science/article/pii/S2352513425001887
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author Xi Shi
Shijie Yuan
Wei Peng
Fengqing Chen
Xiangwei Liu
Xueke Ma
Xiao Ma
Ruwei Xu
Khor Waiho
Xianghui Kong
Cuiyun Lu
Xuejun Li
author_facet Xi Shi
Shijie Yuan
Wei Peng
Fengqing Chen
Xiangwei Liu
Xueke Ma
Xiao Ma
Ruwei Xu
Khor Waiho
Xianghui Kong
Cuiyun Lu
Xuejun Li
author_sort Xi Shi
collection DOAJ
description Saline-alkaline water fishery has emerged as a highly promising aquaculture mode in China. To identify potential species suitable for saline-alkaline water aquaculture, this study investigated the effects of different alkalinity stresses (0, 7, 14, 21, 28 mmol/L) on the survival, growth performance, physiology, and muscle quality of largemouth bass Micropterus salmoides. A total of 450 fish (5.58 ± 0.09 g) were randomly allocated to 15 tanks, with three replicates for each group. The experiment lasted for two months. The results indicated that alkalinity stress exhibited no significant impact on the survival rate but significantly reduced growth performance. Compared with the control group, the ALK-28 group exhibited a significantly higher feed conversion ratio and a relatively lower protein efficiency ratio. Physiologically, as alkalinity increased, the concentrations of Na+ and Mg2+, along with the Na+/K+-ATPase and Ca2+/Mg2+-ATPase activities, showed an upward trend. Histologically, alkalinity stress induced some injuries to gills, including gill lamella hyperplasia, abnormal blood cells accumulation, and chloride cells rupture. For muscle nutrition and quality, no significant differences in the nutritional composition and water-holding capacity among treatments were observed. However, ALK-14 significantly enhanced the flavor and nutritional values of amino acids and fatty acids, and ALK-28 significantly improved textural characteristics, including higher hardness, chewiness, and resilience. These results suggested that largemouth bass is a potentially economic candidate species for saline-alkaline water aquaculture because it can survive and show advantages in muscle nutrition and quality under high alkalinity conditions.
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spelling doaj-art-32482e887f6a48e9a7f9566c5e54d10b2025-08-20T02:31:12ZengElsevierAquaculture Reports2352-51342025-07-014210280210.1016/j.aqrep.2025.102802Responses of largemouth bass (Micropterus salmoides) to chronic alkalinity stress: Survival, growth performance, physiology, and muscle qualityXi Shi0Shijie Yuan1Wei Peng2Fengqing Chen3Xiangwei Liu4Xueke Ma5Xiao Ma6Ruwei Xu7Khor Waiho8Xianghui Kong9Cuiyun Lu10Xuejun Li11College of Fisheries, Henan Normal University, Xinxiang 453007, China; Key Laboratory of Freshwater Aquatic Biotechnology and Breeding, Ministry of Agriculture and Rural Affairs, Harbin 150070, China; College of Life Science, Henan Normal University, Xinxiang 453007, ChinaCollege of Fisheries, Henan Normal University, Xinxiang 453007, ChinaCollege of Fisheries, Henan Normal University, Xinxiang 453007, China; Key Laboratory of Freshwater Aquatic Biotechnology and Breeding, Ministry of Agriculture and Rural Affairs, Harbin 150070, ChinaCollege of Fisheries, Henan Normal University, Xinxiang 453007, ChinaCollege of Fisheries, Henan Normal University, Xinxiang 453007, ChinaCollege of Fisheries, Henan Normal University, Xinxiang 453007, ChinaCollege of Fisheries, Henan Normal University, Xinxiang 453007, ChinaHangzhou Xiaoshan Donghai Breeding Co., Ltd., Hangzhou 311200, ChinaHigher Institution Centre of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu 21030, MalaysiaCollege of Fisheries, Henan Normal University, Xinxiang 453007, China; College of Life Science, Henan Normal University, Xinxiang 453007, ChinaKey Laboratory of Freshwater Aquatic Biotechnology and Breeding, Ministry of Agriculture and Rural Affairs, Harbin 150070, China; Corresponding author.College of Fisheries, Henan Normal University, Xinxiang 453007, China; Observation and Research Station on Water Ecosystem in Danjiangkou Reservoir of Henan Province, Nanyang 474450, China; Corresponding author at: College of Fisheries, Henan Normal University, Xinxiang 453007, China.Saline-alkaline water fishery has emerged as a highly promising aquaculture mode in China. To identify potential species suitable for saline-alkaline water aquaculture, this study investigated the effects of different alkalinity stresses (0, 7, 14, 21, 28 mmol/L) on the survival, growth performance, physiology, and muscle quality of largemouth bass Micropterus salmoides. A total of 450 fish (5.58 ± 0.09 g) were randomly allocated to 15 tanks, with three replicates for each group. The experiment lasted for two months. The results indicated that alkalinity stress exhibited no significant impact on the survival rate but significantly reduced growth performance. Compared with the control group, the ALK-28 group exhibited a significantly higher feed conversion ratio and a relatively lower protein efficiency ratio. Physiologically, as alkalinity increased, the concentrations of Na+ and Mg2+, along with the Na+/K+-ATPase and Ca2+/Mg2+-ATPase activities, showed an upward trend. Histologically, alkalinity stress induced some injuries to gills, including gill lamella hyperplasia, abnormal blood cells accumulation, and chloride cells rupture. For muscle nutrition and quality, no significant differences in the nutritional composition and water-holding capacity among treatments were observed. However, ALK-14 significantly enhanced the flavor and nutritional values of amino acids and fatty acids, and ALK-28 significantly improved textural characteristics, including higher hardness, chewiness, and resilience. These results suggested that largemouth bass is a potentially economic candidate species for saline-alkaline water aquaculture because it can survive and show advantages in muscle nutrition and quality under high alkalinity conditions.http://www.sciencedirect.com/science/article/pii/S2352513425001887Alkalinity stressGrowth performancePhysiologyMuscle qualityLargemouth bass
spellingShingle Xi Shi
Shijie Yuan
Wei Peng
Fengqing Chen
Xiangwei Liu
Xueke Ma
Xiao Ma
Ruwei Xu
Khor Waiho
Xianghui Kong
Cuiyun Lu
Xuejun Li
Responses of largemouth bass (Micropterus salmoides) to chronic alkalinity stress: Survival, growth performance, physiology, and muscle quality
Aquaculture Reports
Alkalinity stress
Growth performance
Physiology
Muscle quality
Largemouth bass
title Responses of largemouth bass (Micropterus salmoides) to chronic alkalinity stress: Survival, growth performance, physiology, and muscle quality
title_full Responses of largemouth bass (Micropterus salmoides) to chronic alkalinity stress: Survival, growth performance, physiology, and muscle quality
title_fullStr Responses of largemouth bass (Micropterus salmoides) to chronic alkalinity stress: Survival, growth performance, physiology, and muscle quality
title_full_unstemmed Responses of largemouth bass (Micropterus salmoides) to chronic alkalinity stress: Survival, growth performance, physiology, and muscle quality
title_short Responses of largemouth bass (Micropterus salmoides) to chronic alkalinity stress: Survival, growth performance, physiology, and muscle quality
title_sort responses of largemouth bass micropterus salmoides to chronic alkalinity stress survival growth performance physiology and muscle quality
topic Alkalinity stress
Growth performance
Physiology
Muscle quality
Largemouth bass
url http://www.sciencedirect.com/science/article/pii/S2352513425001887
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