SIRT3 protects endometrial receptivity in patients with polycystic ovary syndrome
Abstract. Background. The sirtuin family is well recognized for its crucial involvement in various cellular processes. Nevertheless, studies on its role in the human endometrium are limited. This study aimed to explore the expression and localization of the sirtuin family in the human endometrium,...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer
2025-05-01
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| Series: | Chinese Medical Journal |
| Online Access: | http://journals.lww.com/10.1097/CM9.0000000000003127 |
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| author | Zhonghong Zeng Hongying Shan Mingmei Lin Siyu Bao Dan Mo Feng Deng Yang Yu Yihua Yang Ping Zhou Rong Li Yanjie Yin |
| author_facet | Zhonghong Zeng Hongying Shan Mingmei Lin Siyu Bao Dan Mo Feng Deng Yang Yu Yihua Yang Ping Zhou Rong Li Yanjie Yin |
| author_sort | Zhonghong Zeng |
| collection | DOAJ |
| description | Abstract.
Background. The sirtuin family is well recognized for its crucial involvement in various cellular processes. Nevertheless, studies on its role in the human endometrium are limited. This study aimed to explore the expression and localization of the sirtuin family in the human endometrium, focusing on sirtuin 3 (SIRT3) and its potential role in the oxidative imbalance of the endometrium in polycystic ovary syndrome (PCOS).
Methods. Endometrial specimens were collected from both patients with PCOS and controls undergoing hysteroscopy at the Center for Reproductive Medicine, Peking University Third Hospital, from July to August 2015 and used for cell culture. The protective effects of SIRT3 were investigated, and the mechanism of SIRT3 in improving endometrial receptivity of patients with PCOS was determined using various techniques, including cellular bioenergetic analysis, small interfering ribonucleic acid (siRNA) silencing, real-time quantitative polymerase chain reaction, Western blot, immunofluorescence, immunohistochemistry, and flow cytometry analysis.
Results. The sirtuin family was widely expressed in the human endometrium, with SIRT3 showing a significant increase in expression in patients with PCOS compared with controls (P <0.05), as confirmed by protein and gene assays. Concurrently, endometrial antioxidant levels were elevated, while mitochondrial respiratory capacity was reduced, in patients with PCOS (P <0.05). An endometrial oxidative stress (OS) model revealed that the downregulation of SIRT3 impaired the growth and proliferation status of endometrial cells and reduced their receptivity to day 4 mouse embryos. The results suggested that SIRT3 might be crucial in maintaining normal cellular state by regulating antioxidants, cell proliferation, and apoptosis, thereby contributing to enhanced endometrial receptivity.
Conclusions. Our findings proposed a significant role of SIRT3 in improving endometrial receptivity in patients with PCOS by alleviating OS and regulating the balance between cell proliferation and apoptosis. Therefore, SIRT3 could be a promising target for predicting and improving endometrial receptivity in this patient population. |
| format | Article |
| id | doaj-art-3245a0b2e92e489fa51840e3eb54d15d |
| institution | OA Journals |
| issn | 0366-6999 2542-5641 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Wolters Kluwer |
| record_format | Article |
| series | Chinese Medical Journal |
| spelling | doaj-art-3245a0b2e92e489fa51840e3eb54d15d2025-08-20T01:52:16ZengWolters KluwerChinese Medical Journal0366-69992542-56412025-05-01138101225123510.1097/CM9.0000000000003127202505200-00010SIRT3 protects endometrial receptivity in patients with polycystic ovary syndromeZhonghong Zeng0Hongying Shan1Mingmei Lin2Siyu Bao3Dan Mo4Feng Deng5Yang Yu6Yihua Yang7Ping Zhou8Rong Li9Yanjie Yin1 Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Peking University Third Hospital, Beijing 100191, China1 Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Peking University Third Hospital, Beijing 100191, China1 Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Peking University Third Hospital, Beijing 100191, China1 Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Peking University Third Hospital, Beijing 100191, China1 Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Peking University Third Hospital, Beijing 100191, China1 Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Peking University Third Hospital, Beijing 100191, China1 Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Peking University Third Hospital, Beijing 100191, China5 Guangxi Reproductive Medical Center, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, China1 Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Peking University Third Hospital, Beijing 100191, China1 Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Peking University Third Hospital, Beijing 100191, ChinaAbstract. Background. The sirtuin family is well recognized for its crucial involvement in various cellular processes. Nevertheless, studies on its role in the human endometrium are limited. This study aimed to explore the expression and localization of the sirtuin family in the human endometrium, focusing on sirtuin 3 (SIRT3) and its potential role in the oxidative imbalance of the endometrium in polycystic ovary syndrome (PCOS). Methods. Endometrial specimens were collected from both patients with PCOS and controls undergoing hysteroscopy at the Center for Reproductive Medicine, Peking University Third Hospital, from July to August 2015 and used for cell culture. The protective effects of SIRT3 were investigated, and the mechanism of SIRT3 in improving endometrial receptivity of patients with PCOS was determined using various techniques, including cellular bioenergetic analysis, small interfering ribonucleic acid (siRNA) silencing, real-time quantitative polymerase chain reaction, Western blot, immunofluorescence, immunohistochemistry, and flow cytometry analysis. Results. The sirtuin family was widely expressed in the human endometrium, with SIRT3 showing a significant increase in expression in patients with PCOS compared with controls (P <0.05), as confirmed by protein and gene assays. Concurrently, endometrial antioxidant levels were elevated, while mitochondrial respiratory capacity was reduced, in patients with PCOS (P <0.05). An endometrial oxidative stress (OS) model revealed that the downregulation of SIRT3 impaired the growth and proliferation status of endometrial cells and reduced their receptivity to day 4 mouse embryos. The results suggested that SIRT3 might be crucial in maintaining normal cellular state by regulating antioxidants, cell proliferation, and apoptosis, thereby contributing to enhanced endometrial receptivity. Conclusions. Our findings proposed a significant role of SIRT3 in improving endometrial receptivity in patients with PCOS by alleviating OS and regulating the balance between cell proliferation and apoptosis. Therefore, SIRT3 could be a promising target for predicting and improving endometrial receptivity in this patient population.http://journals.lww.com/10.1097/CM9.0000000000003127 |
| spellingShingle | Zhonghong Zeng Hongying Shan Mingmei Lin Siyu Bao Dan Mo Feng Deng Yang Yu Yihua Yang Ping Zhou Rong Li Yanjie Yin SIRT3 protects endometrial receptivity in patients with polycystic ovary syndrome Chinese Medical Journal |
| title | SIRT3 protects endometrial receptivity in patients with polycystic ovary syndrome |
| title_full | SIRT3 protects endometrial receptivity in patients with polycystic ovary syndrome |
| title_fullStr | SIRT3 protects endometrial receptivity in patients with polycystic ovary syndrome |
| title_full_unstemmed | SIRT3 protects endometrial receptivity in patients with polycystic ovary syndrome |
| title_short | SIRT3 protects endometrial receptivity in patients with polycystic ovary syndrome |
| title_sort | sirt3 protects endometrial receptivity in patients with polycystic ovary syndrome |
| url | http://journals.lww.com/10.1097/CM9.0000000000003127 |
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