The Diagnostic and Prognostic Role of Biomarkers in Mild Traumatic Brain Injury: An Umbrella Meta-Analysis

Background: Mild traumatic brain injury (mTBI), commonly known as concussion, is a major public health issue characterized by subtle neuronal damage that traditional imaging techniques such as computed tomography (CT) and magnetic resonance imaging (MRI) often fail to detect. Fluid biomarkers have e...

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Main Authors: Ioannis Mavroudis, Foivos Petridis, Dimitrios Kazis, Alin Ciobica, Gabriel Dăscălescu, Antoneta Dacia Petroaie, Irina Dobrin, Otilia Novac, Ioana Vata, Bogdan Novac
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Language:English
Published: MDPI AG 2025-05-01
Series:Brain Sciences
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Online Access:https://www.mdpi.com/2076-3425/15/6/581
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author Ioannis Mavroudis
Foivos Petridis
Dimitrios Kazis
Alin Ciobica
Gabriel Dăscălescu
Antoneta Dacia Petroaie
Irina Dobrin
Otilia Novac
Ioana Vata
Bogdan Novac
author_facet Ioannis Mavroudis
Foivos Petridis
Dimitrios Kazis
Alin Ciobica
Gabriel Dăscălescu
Antoneta Dacia Petroaie
Irina Dobrin
Otilia Novac
Ioana Vata
Bogdan Novac
author_sort Ioannis Mavroudis
collection DOAJ
description Background: Mild traumatic brain injury (mTBI), commonly known as concussion, is a major public health issue characterized by subtle neuronal damage that traditional imaging techniques such as computed tomography (CT) and magnetic resonance imaging (MRI) often fail to detect. Fluid biomarkers have emerged as promising diagnostic and prognostic tools for mTBI. Objectives: This umbrella meta-analysis aims to evaluate the diagnostic accuracy and clinical utility of the key fluid biomarkers, S100B, glial fibrillary acidic protein (GFAP), ubiquitin carboxy-terminal hydrolase L1 (UCH-L1, neurofilament light chain (NfL)) and tau protein, in detecting mTBI and to clarify their roles as screening, confirmatory, and complementary indicators. Methods: A systematic review was performed using PubMed, Web of Science, Scopus, and Cochrane to identify the published meta-analyses that assessed the biomarkers in mTBI. Sensitivity, specificity, and diagnostic odds ratios were then calculated using random-effects models. Heterogeneity was evaluated with the I<sup>2</sup> statistic, and publication bias was assessed via funnel plots. The results of S100B demonstrated high sensitivity (91.6%) but low specificity (42.4%), making it an effective rule-out biomarker to minimize unnecessary CT scans. In contrast, GFAP exhibited moderate sensitivity (84.5%) with improved specificity (61.0%), supporting its role in confirming mTBI diagnoses. UCH-L1 revealed a sensitivity of 86.7% alongside low specificity (37.3%), indicating its potential as a complementary marker. Additionally, the NfL levels were notably elevated in sports-related concussions, while the diagnostic utility of tau protein remains inconclusive due to limited available data. Conclusions: The findings underscore the clinical promise of fluid biomarkers in the management of mTBI. S100B and GFAP are particularly valuable as screening and confirmatory markers, respectively. Nonetheless, further standardization of biomarker thresholds and additional longitudinal studies are necessary to validate the roles of UCH-L1, NfL, and Tau protein. The integration of these biomarkers into a multimodal diagnostic panel may enhance mTBI detection accuracy and facilitate improved patient stratification and management.
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spelling doaj-art-323f1d2fcad14e27b618e4bf065e348f2025-08-20T03:27:13ZengMDPI AGBrain Sciences2076-34252025-05-0115658110.3390/brainsci15060581The Diagnostic and Prognostic Role of Biomarkers in Mild Traumatic Brain Injury: An Umbrella Meta-AnalysisIoannis Mavroudis0Foivos Petridis1Dimitrios Kazis2Alin Ciobica3Gabriel Dăscălescu4Antoneta Dacia Petroaie5Irina Dobrin6Otilia Novac7Ioana Vata8Bogdan Novac9Department of Neuroscience, Leeds Teaching Hospitals, NHS Trust, Leeds LS9 7TF, UKThird Department of Neurology, Aristotle University of Thessaloniki, 541 24 Thessaloniki, GreeceThird Department of Neurology, Aristotle University of Thessaloniki, 541 24 Thessaloniki, GreeceFaculty of Biology, “Alexandru Ioan Cuza” University of Iași, 700506 Iași, RomaniaFaculty of Biology, “Alexandru Ioan Cuza” University of Iași, 700506 Iași, RomaniaFaculty of Medicine, University of Medicine and Pharmacy “Grigore T. Popa”, 700115 Iasi, RomaniaFaculty of Medicine, University of Medicine and Pharmacy “Grigore T. Popa”, 700115 Iasi, RomaniaFaculty of Medicine, University of Medicine and Pharmacy “Grigore T. Popa”, 700115 Iasi, RomaniaFaculty of Dental Medicine, Apollonia University, Pacurari Street 11, 700511 Iași, RomaniaFaculty of Medicine, University of Medicine and Pharmacy “Grigore T. Popa”, 700115 Iasi, RomaniaBackground: Mild traumatic brain injury (mTBI), commonly known as concussion, is a major public health issue characterized by subtle neuronal damage that traditional imaging techniques such as computed tomography (CT) and magnetic resonance imaging (MRI) often fail to detect. Fluid biomarkers have emerged as promising diagnostic and prognostic tools for mTBI. Objectives: This umbrella meta-analysis aims to evaluate the diagnostic accuracy and clinical utility of the key fluid biomarkers, S100B, glial fibrillary acidic protein (GFAP), ubiquitin carboxy-terminal hydrolase L1 (UCH-L1, neurofilament light chain (NfL)) and tau protein, in detecting mTBI and to clarify their roles as screening, confirmatory, and complementary indicators. Methods: A systematic review was performed using PubMed, Web of Science, Scopus, and Cochrane to identify the published meta-analyses that assessed the biomarkers in mTBI. Sensitivity, specificity, and diagnostic odds ratios were then calculated using random-effects models. Heterogeneity was evaluated with the I<sup>2</sup> statistic, and publication bias was assessed via funnel plots. The results of S100B demonstrated high sensitivity (91.6%) but low specificity (42.4%), making it an effective rule-out biomarker to minimize unnecessary CT scans. In contrast, GFAP exhibited moderate sensitivity (84.5%) with improved specificity (61.0%), supporting its role in confirming mTBI diagnoses. UCH-L1 revealed a sensitivity of 86.7% alongside low specificity (37.3%), indicating its potential as a complementary marker. Additionally, the NfL levels were notably elevated in sports-related concussions, while the diagnostic utility of tau protein remains inconclusive due to limited available data. Conclusions: The findings underscore the clinical promise of fluid biomarkers in the management of mTBI. S100B and GFAP are particularly valuable as screening and confirmatory markers, respectively. Nonetheless, further standardization of biomarker thresholds and additional longitudinal studies are necessary to validate the roles of UCH-L1, NfL, and Tau protein. The integration of these biomarkers into a multimodal diagnostic panel may enhance mTBI detection accuracy and facilitate improved patient stratification and management.https://www.mdpi.com/2076-3425/15/6/581mild traumatic brain injuryconcussionbiomarkersS100BGFAPUCH-L1
spellingShingle Ioannis Mavroudis
Foivos Petridis
Dimitrios Kazis
Alin Ciobica
Gabriel Dăscălescu
Antoneta Dacia Petroaie
Irina Dobrin
Otilia Novac
Ioana Vata
Bogdan Novac
The Diagnostic and Prognostic Role of Biomarkers in Mild Traumatic Brain Injury: An Umbrella Meta-Analysis
Brain Sciences
mild traumatic brain injury
concussion
biomarkers
S100B
GFAP
UCH-L1
title The Diagnostic and Prognostic Role of Biomarkers in Mild Traumatic Brain Injury: An Umbrella Meta-Analysis
title_full The Diagnostic and Prognostic Role of Biomarkers in Mild Traumatic Brain Injury: An Umbrella Meta-Analysis
title_fullStr The Diagnostic and Prognostic Role of Biomarkers in Mild Traumatic Brain Injury: An Umbrella Meta-Analysis
title_full_unstemmed The Diagnostic and Prognostic Role of Biomarkers in Mild Traumatic Brain Injury: An Umbrella Meta-Analysis
title_short The Diagnostic and Prognostic Role of Biomarkers in Mild Traumatic Brain Injury: An Umbrella Meta-Analysis
title_sort diagnostic and prognostic role of biomarkers in mild traumatic brain injury an umbrella meta analysis
topic mild traumatic brain injury
concussion
biomarkers
S100B
GFAP
UCH-L1
url https://www.mdpi.com/2076-3425/15/6/581
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