Evaluation of <i>Kabuli</i> Chickpea Genotypes for Terminal Drought Tolerance in Tropical Growing Environment

Terminal drought is the major constraint for chickpea production, leading to yield losses of up to 90% in tropical environments. Understanding the morphological, phenological, and physiological traits underlying drought tolerance is crucial for developing resilient chickpea genotypes. This study elu...

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Main Authors: Megha Subedi, Mani Naiker, Ryan du Preez, Dante L. Adorada, Surya Bhattarai
Format: Article
Language:English
Published: MDPI AG 2025-03-01
Series:Plants
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Online Access:https://www.mdpi.com/2223-7747/14/5/806
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author Megha Subedi
Mani Naiker
Ryan du Preez
Dante L. Adorada
Surya Bhattarai
author_facet Megha Subedi
Mani Naiker
Ryan du Preez
Dante L. Adorada
Surya Bhattarai
author_sort Megha Subedi
collection DOAJ
description Terminal drought is the major constraint for chickpea production, leading to yield losses of up to 90% in tropical environments. Understanding the morphological, phenological, and physiological traits underlying drought tolerance is crucial for developing resilient chickpea genotypes. This study elucidates the drought-tolerant traits of eight <i>kabuli</i> chickpea genotypes under a controlled environment using polyvinyl chloride (PVC) lysimeters. Terminal drought was imposed after the flowering stage, and the response was assessed against non-stress (well-watered) treatment. Drought stress significantly impacted gas-exchange parameters, reducing the stomatal conductance (16–35%), chlorophyll content (10–22%), carbon assimilation rate (21–40%) and internal carbon concentration (7–14%). Principal component analysis (PCA) indicated three groups among these eight genotypes. The drought-tolerant group included two genotypes (AVTCPK#6 and AVTCPK#19) with higher water use efficiency (WUE), deep-rooted plants, longer maturity, and seed yield stability under drought stress. In contrast, the drought-susceptible group included two genotypes (AVTCPK#1 and AVTCPK#12) that were early-maturing and low-yielding with poor assimilation rates. The intermediate group included four genotypes (AVTCPK#3, AVTCPK8, AVTCPK#24, and AVTCPK#25) that exhibited medium maturity and medium yield, conferring intermediate tolerance to terminal drought. A significantly strong positive correlation was observed between seed yield and key physiological traits (stomatal conductance (gsw), leaf chlorophyll content (SPAD) and carbon assimilation rate (A<sub>sat</sub>)) and morphological traits (plant height, number of pods, and root biomass). Conversely, carbon discrimination (Δ<sup>13</sup>C) and intrinsic WUE (iWUE) showed a strong negative correlation with seed yield, supporting Δ<sup>13</sup>C as a surrogate for WUE and drought tolerance and a trait suitable for the selection of <i>kabuli</i> chickpea genotypes for drought resilience.
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spelling doaj-art-323e61b326f84a6fa277effdcc0ef0302025-08-20T02:59:00ZengMDPI AGPlants2223-77472025-03-0114580610.3390/plants14050806Evaluation of <i>Kabuli</i> Chickpea Genotypes for Terminal Drought Tolerance in Tropical Growing EnvironmentMegha Subedi0Mani Naiker1Ryan du Preez2Dante L. Adorada3Surya Bhattarai4School of Health, Medical and Applied Sciences, Central Queensland University, Rockhampton, QLD 4701, AustraliaSchool of Health, Medical and Applied Sciences, Central Queensland University, Rockhampton, QLD 4701, AustraliaSchool of Health, Medical and Applied Sciences, Central Queensland University, Rockhampton, QLD 4701, AustraliaCentre for Crop Health, Institute for Life Science and the Environment, University of Southern Queensland, Toowoomba, QLD 4350, AustraliaSchool of Health, Medical and Applied Sciences, Central Queensland University, Rockhampton, QLD 4701, AustraliaTerminal drought is the major constraint for chickpea production, leading to yield losses of up to 90% in tropical environments. Understanding the morphological, phenological, and physiological traits underlying drought tolerance is crucial for developing resilient chickpea genotypes. This study elucidates the drought-tolerant traits of eight <i>kabuli</i> chickpea genotypes under a controlled environment using polyvinyl chloride (PVC) lysimeters. Terminal drought was imposed after the flowering stage, and the response was assessed against non-stress (well-watered) treatment. Drought stress significantly impacted gas-exchange parameters, reducing the stomatal conductance (16–35%), chlorophyll content (10–22%), carbon assimilation rate (21–40%) and internal carbon concentration (7–14%). Principal component analysis (PCA) indicated three groups among these eight genotypes. The drought-tolerant group included two genotypes (AVTCPK#6 and AVTCPK#19) with higher water use efficiency (WUE), deep-rooted plants, longer maturity, and seed yield stability under drought stress. In contrast, the drought-susceptible group included two genotypes (AVTCPK#1 and AVTCPK#12) that were early-maturing and low-yielding with poor assimilation rates. The intermediate group included four genotypes (AVTCPK#3, AVTCPK8, AVTCPK#24, and AVTCPK#25) that exhibited medium maturity and medium yield, conferring intermediate tolerance to terminal drought. A significantly strong positive correlation was observed between seed yield and key physiological traits (stomatal conductance (gsw), leaf chlorophyll content (SPAD) and carbon assimilation rate (A<sub>sat</sub>)) and morphological traits (plant height, number of pods, and root biomass). Conversely, carbon discrimination (Δ<sup>13</sup>C) and intrinsic WUE (iWUE) showed a strong negative correlation with seed yield, supporting Δ<sup>13</sup>C as a surrogate for WUE and drought tolerance and a trait suitable for the selection of <i>kabuli</i> chickpea genotypes for drought resilience.https://www.mdpi.com/2223-7747/14/5/806<i>kabuli</i>chickpeaterminal droughttropical adaptation
spellingShingle Megha Subedi
Mani Naiker
Ryan du Preez
Dante L. Adorada
Surya Bhattarai
Evaluation of <i>Kabuli</i> Chickpea Genotypes for Terminal Drought Tolerance in Tropical Growing Environment
Plants
<i>kabuli</i>
chickpea
terminal drought
tropical adaptation
title Evaluation of <i>Kabuli</i> Chickpea Genotypes for Terminal Drought Tolerance in Tropical Growing Environment
title_full Evaluation of <i>Kabuli</i> Chickpea Genotypes for Terminal Drought Tolerance in Tropical Growing Environment
title_fullStr Evaluation of <i>Kabuli</i> Chickpea Genotypes for Terminal Drought Tolerance in Tropical Growing Environment
title_full_unstemmed Evaluation of <i>Kabuli</i> Chickpea Genotypes for Terminal Drought Tolerance in Tropical Growing Environment
title_short Evaluation of <i>Kabuli</i> Chickpea Genotypes for Terminal Drought Tolerance in Tropical Growing Environment
title_sort evaluation of i kabuli i chickpea genotypes for terminal drought tolerance in tropical growing environment
topic <i>kabuli</i>
chickpea
terminal drought
tropical adaptation
url https://www.mdpi.com/2223-7747/14/5/806
work_keys_str_mv AT meghasubedi evaluationofikabuliichickpeagenotypesforterminaldroughttoleranceintropicalgrowingenvironment
AT maninaiker evaluationofikabuliichickpeagenotypesforterminaldroughttoleranceintropicalgrowingenvironment
AT ryandupreez evaluationofikabuliichickpeagenotypesforterminaldroughttoleranceintropicalgrowingenvironment
AT danteladorada evaluationofikabuliichickpeagenotypesforterminaldroughttoleranceintropicalgrowingenvironment
AT suryabhattarai evaluationofikabuliichickpeagenotypesforterminaldroughttoleranceintropicalgrowingenvironment