Proteomic profile of human sinoatrial and atrioventricular nodes in comparison to working myocardium
Abstract The proteomic profile of the human cardiac conduction system: the sinoatrial node (SAN) and atrioventricular node (AVN), remains poorly understood. The aim of the current study is to identify proteomic characteristic of the human SAN and AVN in the comparison to working myocardium of the ri...
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Nature Portfolio
2025-02-01
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| Online Access: | https://doi.org/10.1038/s41598-025-89255-y |
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| author | Agata Krawczyk-Ożóg Aneta Stachowicz Grzegorz Szoniec Jakub Batko Kamila Stachyra Filip Bolechała Marcin Strona Paweł P. Wołkow Zeyuan Yin Halina Dobrzynski Mateusz K. Hołda |
| author_facet | Agata Krawczyk-Ożóg Aneta Stachowicz Grzegorz Szoniec Jakub Batko Kamila Stachyra Filip Bolechała Marcin Strona Paweł P. Wołkow Zeyuan Yin Halina Dobrzynski Mateusz K. Hołda |
| author_sort | Agata Krawczyk-Ożóg |
| collection | DOAJ |
| description | Abstract The proteomic profile of the human cardiac conduction system: the sinoatrial node (SAN) and atrioventricular node (AVN), remains poorly understood. The aim of the current study is to identify proteomic characteristic of the human SAN and AVN in the comparison to working myocardium of the right atrium (RAM) and right ventricle (RVM). The proteomic analysis was performed on 10 autopsied human heart specimens collected from healthy adults. During the data-independent acquisition proteomics analysis 2752 different proteins were identified in all sample sets. In both nodal tissues (compared to working myocardium), the following pathways were upregulated: regulation of Insulin-like Growth Factor transport and uptake by Insulin-like Growth Factor Binding Proteins, post-translational protein phosphorylation, glutathione metabolism, metabolism of carbohydrates, glycolysis and gluconeogenesis. Other common for nodal tissue pathways were these related to immune system and related to extracellular matrix. The pathways related to cardiac muscle contraction were more abundant in RAM and RVM samples. The current study presents extensive comparative analysis of protein abundance in the human SAN and AVN. Few key differences may be found in the nodal proteome in comparison to working cardiomyocytes, including involvement of immune system and upregulated pathways related to extracellular matrix. The SAN exhibits enrichment in the PPAR signaling and pentose phosphate pathways, as well as prostaglandin synthesis and regulatory proteins, compared to the AVN. |
| format | Article |
| id | doaj-art-322f06407ff54125b6cf5bfe4b2cb6d9 |
| institution | DOAJ |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-322f06407ff54125b6cf5bfe4b2cb6d92025-08-20T03:05:18ZengNature PortfolioScientific Reports2045-23222025-02-0115111410.1038/s41598-025-89255-yProteomic profile of human sinoatrial and atrioventricular nodes in comparison to working myocardiumAgata Krawczyk-Ożóg0Aneta Stachowicz1Grzegorz Szoniec2Jakub Batko3Kamila Stachyra4Filip Bolechała5Marcin Strona6Paweł P. Wołkow7Zeyuan Yin8Halina Dobrzynski9Mateusz K. Hołda10HEART - Heart Embryology and Anatomy Research Team, Department of Anatomy, Jagiellonian University Medical CollegeDepartment of Pharmacology, Faculty of Medicine, Jagiellonian University Medical CollegeCenter for Medical Genomics OMICRON, Faculty of Medicine, Jagiellonian University Medical CollegeHEART - Heart Embryology and Anatomy Research Team, Department of Anatomy, Jagiellonian University Medical CollegeDepartment of Pharmacology, Faculty of Medicine, Jagiellonian University Medical CollegeDepartment of Forensic Medicine, Jagiellonian University Medical CollegeDepartment of Forensic Medicine, Jagiellonian University Medical CollegeDivision of Laboratory Diagnostics and Clinical Epigenetics, Faculty of Medicine, Institute of Medical Sciences, University of Rzeszów Medical CollegeDivision of Cardiovascular Sciences, The University of ManchesterHEART - Heart Embryology and Anatomy Research Team, Department of Anatomy, Jagiellonian University Medical CollegeHEART - Heart Embryology and Anatomy Research Team, Department of Anatomy, Jagiellonian University Medical CollegeAbstract The proteomic profile of the human cardiac conduction system: the sinoatrial node (SAN) and atrioventricular node (AVN), remains poorly understood. The aim of the current study is to identify proteomic characteristic of the human SAN and AVN in the comparison to working myocardium of the right atrium (RAM) and right ventricle (RVM). The proteomic analysis was performed on 10 autopsied human heart specimens collected from healthy adults. During the data-independent acquisition proteomics analysis 2752 different proteins were identified in all sample sets. In both nodal tissues (compared to working myocardium), the following pathways were upregulated: regulation of Insulin-like Growth Factor transport and uptake by Insulin-like Growth Factor Binding Proteins, post-translational protein phosphorylation, glutathione metabolism, metabolism of carbohydrates, glycolysis and gluconeogenesis. Other common for nodal tissue pathways were these related to immune system and related to extracellular matrix. The pathways related to cardiac muscle contraction were more abundant in RAM and RVM samples. The current study presents extensive comparative analysis of protein abundance in the human SAN and AVN. Few key differences may be found in the nodal proteome in comparison to working cardiomyocytes, including involvement of immune system and upregulated pathways related to extracellular matrix. The SAN exhibits enrichment in the PPAR signaling and pentose phosphate pathways, as well as prostaglandin synthesis and regulatory proteins, compared to the AVN.https://doi.org/10.1038/s41598-025-89255-yAtrioventricular nodeProteomicsRight atriumRight ventricleSinoatrial node |
| spellingShingle | Agata Krawczyk-Ożóg Aneta Stachowicz Grzegorz Szoniec Jakub Batko Kamila Stachyra Filip Bolechała Marcin Strona Paweł P. Wołkow Zeyuan Yin Halina Dobrzynski Mateusz K. Hołda Proteomic profile of human sinoatrial and atrioventricular nodes in comparison to working myocardium Scientific Reports Atrioventricular node Proteomics Right atrium Right ventricle Sinoatrial node |
| title | Proteomic profile of human sinoatrial and atrioventricular nodes in comparison to working myocardium |
| title_full | Proteomic profile of human sinoatrial and atrioventricular nodes in comparison to working myocardium |
| title_fullStr | Proteomic profile of human sinoatrial and atrioventricular nodes in comparison to working myocardium |
| title_full_unstemmed | Proteomic profile of human sinoatrial and atrioventricular nodes in comparison to working myocardium |
| title_short | Proteomic profile of human sinoatrial and atrioventricular nodes in comparison to working myocardium |
| title_sort | proteomic profile of human sinoatrial and atrioventricular nodes in comparison to working myocardium |
| topic | Atrioventricular node Proteomics Right atrium Right ventricle Sinoatrial node |
| url | https://doi.org/10.1038/s41598-025-89255-y |
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