Phytochemical Screening, Pharmacognostic Characterization, Antioxidant Activity, and Hepatoprotective Effects of <i>Abroma augustum</i> (L.) L.f. on Human Hepatocellular Carcinoma (HepG2) Cells and Goat Liver Homogenate
<i>Abroma augustum</i> (L.) L. f. is characterized by its fibrous structure, spiny trichomes, and distinctive leaf formations, which collectively contribute to its unique morphology and potential medicinal applications. This study aims to investigate the phytochemical constituents and el...
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2025-04-01
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| author | Sandipan Das Tanushree Deb Filomena Mottola Nithar Ranjan Madhu Yogisharadhya Revanaiah Israel Maldonado Rosas Sarbani Dey Ray Shubhadeep Roychoudhury |
| author_facet | Sandipan Das Tanushree Deb Filomena Mottola Nithar Ranjan Madhu Yogisharadhya Revanaiah Israel Maldonado Rosas Sarbani Dey Ray Shubhadeep Roychoudhury |
| author_sort | Sandipan Das |
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| description | <i>Abroma augustum</i> (L.) L. f. is characterized by its fibrous structure, spiny trichomes, and distinctive leaf formations, which collectively contribute to its unique morphology and potential medicinal applications. This study aims to investigate the phytochemical constituents and elucidate the pharmacognostic and physicochemical characteristics of the stem bark powder, including evaluation of its antioxidant capacity and hepatoprotective effects against carbon tetrachloride (CCl<sub>4</sub>)-induced hepatotoxicity in both in vitro and ex vivo experimental models. Comprehensive phytochemical screening identified 50 distinct phytochemicals, including a range of alkaloids, flavonoids, terpenes, phenolics, and coumarins, among others. The extract displayed substantial solubility, with total phenolic and flavonoid content quantified as 12.32 ± 0.01 mg/g and 42.14 ± 3.5 mg/g, respectively. The antioxidant activity revealed IC<sub>50</sub> values obtained from 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), and 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic) acid (ABTS), measured at 214.007 µg/mL, 132.307 µg/mL, and 45.455 µg/mL, respectively. Additionally, the methanolic extract exhibited significant hepatoprotective properties, with observable reductions in lipid peroxidation and decreased concentrations of liver damage biomarkers (ALT, AST, and LDH) in both HepG2 cells and goat liver homogenate. Future investigations are warranted to elucidate the underlying mechanisms of these effects, including histopathological examinations and biochemical assays, followed by the administration of plant methanolic extracts. |
| format | Article |
| id | doaj-art-32286d76c8db4793b837441f1f00d44d |
| institution | OA Journals |
| issn | 2076-3921 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj-art-32286d76c8db4793b837441f1f00d44d2025-08-20T02:17:19ZengMDPI AGAntioxidants2076-39212025-04-0114447210.3390/antiox14040472Phytochemical Screening, Pharmacognostic Characterization, Antioxidant Activity, and Hepatoprotective Effects of <i>Abroma augustum</i> (L.) L.f. on Human Hepatocellular Carcinoma (HepG2) Cells and Goat Liver HomogenateSandipan Das0Tanushree Deb1Filomena Mottola2Nithar Ranjan Madhu3Yogisharadhya Revanaiah4Israel Maldonado Rosas5Sarbani Dey Ray6Shubhadeep Roychoudhury7Department of Life Science and Bioinformatics, Assam University, Silchar 788011, IndiaDepartment of Medicine, Silchar Medical College, Silchar 788014, IndiaDepartment of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania Luigi Vanvitelli, 81100 Caserta, ItalyDepartment of Zoology, Acharya Prafulla Chandra College, New Barrackpore 700131, IndiaICAR-Krishi Vigyan Kendra Hailakandi, ICAR Research Complex for NEH Region, Hailakandi 788152, IndiaCitmer Reproductive Medicine, Mexico City 11520, MexicoDepartment of Pharmaceutical Sciences, Assam University, Silchar 788011, IndiaDepartment of Life Science and Bioinformatics, Assam University, Silchar 788011, India<i>Abroma augustum</i> (L.) L. f. is characterized by its fibrous structure, spiny trichomes, and distinctive leaf formations, which collectively contribute to its unique morphology and potential medicinal applications. This study aims to investigate the phytochemical constituents and elucidate the pharmacognostic and physicochemical characteristics of the stem bark powder, including evaluation of its antioxidant capacity and hepatoprotective effects against carbon tetrachloride (CCl<sub>4</sub>)-induced hepatotoxicity in both in vitro and ex vivo experimental models. Comprehensive phytochemical screening identified 50 distinct phytochemicals, including a range of alkaloids, flavonoids, terpenes, phenolics, and coumarins, among others. The extract displayed substantial solubility, with total phenolic and flavonoid content quantified as 12.32 ± 0.01 mg/g and 42.14 ± 3.5 mg/g, respectively. The antioxidant activity revealed IC<sub>50</sub> values obtained from 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), and 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic) acid (ABTS), measured at 214.007 µg/mL, 132.307 µg/mL, and 45.455 µg/mL, respectively. Additionally, the methanolic extract exhibited significant hepatoprotective properties, with observable reductions in lipid peroxidation and decreased concentrations of liver damage biomarkers (ALT, AST, and LDH) in both HepG2 cells and goat liver homogenate. Future investigations are warranted to elucidate the underlying mechanisms of these effects, including histopathological examinations and biochemical assays, followed by the administration of plant methanolic extracts.https://www.mdpi.com/2076-3921/14/4/472Devil’s cottonmethanolic extractphenolicsflavonoidsoxidative stress |
| spellingShingle | Sandipan Das Tanushree Deb Filomena Mottola Nithar Ranjan Madhu Yogisharadhya Revanaiah Israel Maldonado Rosas Sarbani Dey Ray Shubhadeep Roychoudhury Phytochemical Screening, Pharmacognostic Characterization, Antioxidant Activity, and Hepatoprotective Effects of <i>Abroma augustum</i> (L.) L.f. on Human Hepatocellular Carcinoma (HepG2) Cells and Goat Liver Homogenate Antioxidants Devil’s cotton methanolic extract phenolics flavonoids oxidative stress |
| title | Phytochemical Screening, Pharmacognostic Characterization, Antioxidant Activity, and Hepatoprotective Effects of <i>Abroma augustum</i> (L.) L.f. on Human Hepatocellular Carcinoma (HepG2) Cells and Goat Liver Homogenate |
| title_full | Phytochemical Screening, Pharmacognostic Characterization, Antioxidant Activity, and Hepatoprotective Effects of <i>Abroma augustum</i> (L.) L.f. on Human Hepatocellular Carcinoma (HepG2) Cells and Goat Liver Homogenate |
| title_fullStr | Phytochemical Screening, Pharmacognostic Characterization, Antioxidant Activity, and Hepatoprotective Effects of <i>Abroma augustum</i> (L.) L.f. on Human Hepatocellular Carcinoma (HepG2) Cells and Goat Liver Homogenate |
| title_full_unstemmed | Phytochemical Screening, Pharmacognostic Characterization, Antioxidant Activity, and Hepatoprotective Effects of <i>Abroma augustum</i> (L.) L.f. on Human Hepatocellular Carcinoma (HepG2) Cells and Goat Liver Homogenate |
| title_short | Phytochemical Screening, Pharmacognostic Characterization, Antioxidant Activity, and Hepatoprotective Effects of <i>Abroma augustum</i> (L.) L.f. on Human Hepatocellular Carcinoma (HepG2) Cells and Goat Liver Homogenate |
| title_sort | phytochemical screening pharmacognostic characterization antioxidant activity and hepatoprotective effects of i abroma augustum i l l f on human hepatocellular carcinoma hepg2 cells and goat liver homogenate |
| topic | Devil’s cotton methanolic extract phenolics flavonoids oxidative stress |
| url | https://www.mdpi.com/2076-3921/14/4/472 |
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