XYLEM NAC DOMAIN 1 (EjXND1) relieves cold-induced lignification by negatively regulating the EjHB1-EjPRX12 module in loquat fruit
Introduction: Lignin is a principal constituent of the secondary cell wall, which plays a role in both plant growth and defensing against stress, such as low temperature and pest infestation. Additionally, it also accumulates in fleshy fruits and negatively affects fruit quality. Red-fleshed loquat...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-07-01
|
| Series: | Journal of Advanced Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S209012322400376X |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849329479769391104 |
|---|---|
| author | Zihao Liang Yanna Shi Yiqing Huang Jiao Lu Mengxue Zhang Xizhi Cao Ruoqian Hu Dongdong Li Wenbo Chen Changqing Zhu Di Wu Kunsong Chen |
| author_facet | Zihao Liang Yanna Shi Yiqing Huang Jiao Lu Mengxue Zhang Xizhi Cao Ruoqian Hu Dongdong Li Wenbo Chen Changqing Zhu Di Wu Kunsong Chen |
| author_sort | Zihao Liang |
| collection | DOAJ |
| description | Introduction: Lignin is a principal constituent of the secondary cell wall, which plays a role in both plant growth and defensing against stress, such as low temperature and pest infestation. Additionally, it also accumulates in fleshy fruits and negatively affects fruit quality. Red-fleshed loquat is temperature sensitive and exhibits cold-induced lignification. A number of technologies have been developed, for example, Low Temperature Conditioning (LTC) treatment, which has been applied in order to relieve the symptom of cold injury. Objectives: The present study seeks to elucidate the regulatory mechanism underlying cold-induced lignification in loquat fruit. Methods: The target genes were isolated through the analysis of transcriptome. The gene function was analyzed by transient transgenic method in tobacco leaves and loquat fruit, respectively, as well as stable overexpression in liverwort. The regulatory mechanism study was achieved by in vitro protein–protein interaction assays, dual-luciferase assay, and EMSA. Results: In the present study, the Xylem NAC Domain transcription factor EjXND1 was identified as a repressor of loquat fruit lignification. It was demonstrated that EjXND1 could interact with the characterized lignin activator EjHB1, resulting in a diminution of the activation of EjHB1 on EjPRX12 promoter. Furthermore, two highly methylated regions were identified in the promoter of EjXDN1. One of these regions exhibited a negative correlation between methylation level and EjXND1 expression. Additionally, it was shown that hypermethylation of this region weaken the binding affinity of EjXND1 activators to its promoter. Conclusion: The EjXND1 plays a role in modified Low Temperature Conditioning (mLTC) treatment that alleviates cold-induced lignification in red-fleshed loquat fruit by targeting the EjHB1-EjPRX12 module and EjXND1 is regulated by the dynamic of DNA methylation level in the promoter. |
| format | Article |
| id | doaj-art-321a67d2acd3433d8eb0b08044e2815a |
| institution | Kabale University |
| issn | 2090-1232 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Advanced Research |
| spelling | doaj-art-321a67d2acd3433d8eb0b08044e2815a2025-08-20T03:47:16ZengElsevierJournal of Advanced Research2090-12322025-07-01739310410.1016/j.jare.2024.08.032XYLEM NAC DOMAIN 1 (EjXND1) relieves cold-induced lignification by negatively regulating the EjHB1-EjPRX12 module in loquat fruitZihao Liang0Yanna Shi1Yiqing Huang2Jiao Lu3Mengxue Zhang4Xizhi Cao5Ruoqian Hu6Dongdong Li7Wenbo Chen8Changqing Zhu9Di Wu10Kunsong Chen11College of Agriculture and Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, ChinaCollege of Agriculture and Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, China; Zhejiang Key Laboratory of Horticultural Crop Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China; The State Agriculture Ministry Laboratory of Horticultural Plant Growth and Development, Zhejiang University, Zijingang Campus, Hangzhou 310058, ChinaCollege of Agriculture and Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, ChinaCollege of Agriculture and Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, ChinaCollege of Agriculture and Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, ChinaCollege of Agriculture and Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, ChinaCollege of Agriculture and Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, ChinaCollege of Agriculture and Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, China; Zhejiang Key Laboratory of Horticultural Crop Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China; The State Agriculture Ministry Laboratory of Horticultural Plant Growth and Development, Zhejiang University, Zijingang Campus, Hangzhou 310058, ChinaCollege of Agriculture and Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, China; Zhejiang Key Laboratory of Horticultural Crop Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China; The State Agriculture Ministry Laboratory of Horticultural Plant Growth and Development, Zhejiang University, Zijingang Campus, Hangzhou 310058, ChinaCollege of Agriculture and Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, China; Zhejiang Key Laboratory of Horticultural Crop Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China; The State Agriculture Ministry Laboratory of Horticultural Plant Growth and Development, Zhejiang University, Zijingang Campus, Hangzhou 310058, ChinaCollege of Agriculture and Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, China; Zhejiang Key Laboratory of Horticultural Crop Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China; The State Agriculture Ministry Laboratory of Horticultural Plant Growth and Development, Zhejiang University, Zijingang Campus, Hangzhou 310058, ChinaCollege of Agriculture and Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, China; Zhejiang Key Laboratory of Horticultural Crop Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China; The State Agriculture Ministry Laboratory of Horticultural Plant Growth and Development, Zhejiang University, Zijingang Campus, Hangzhou 310058, China; Corresponding author.Introduction: Lignin is a principal constituent of the secondary cell wall, which plays a role in both plant growth and defensing against stress, such as low temperature and pest infestation. Additionally, it also accumulates in fleshy fruits and negatively affects fruit quality. Red-fleshed loquat is temperature sensitive and exhibits cold-induced lignification. A number of technologies have been developed, for example, Low Temperature Conditioning (LTC) treatment, which has been applied in order to relieve the symptom of cold injury. Objectives: The present study seeks to elucidate the regulatory mechanism underlying cold-induced lignification in loquat fruit. Methods: The target genes were isolated through the analysis of transcriptome. The gene function was analyzed by transient transgenic method in tobacco leaves and loquat fruit, respectively, as well as stable overexpression in liverwort. The regulatory mechanism study was achieved by in vitro protein–protein interaction assays, dual-luciferase assay, and EMSA. Results: In the present study, the Xylem NAC Domain transcription factor EjXND1 was identified as a repressor of loquat fruit lignification. It was demonstrated that EjXND1 could interact with the characterized lignin activator EjHB1, resulting in a diminution of the activation of EjHB1 on EjPRX12 promoter. Furthermore, two highly methylated regions were identified in the promoter of EjXDN1. One of these regions exhibited a negative correlation between methylation level and EjXND1 expression. Additionally, it was shown that hypermethylation of this region weaken the binding affinity of EjXND1 activators to its promoter. Conclusion: The EjXND1 plays a role in modified Low Temperature Conditioning (mLTC) treatment that alleviates cold-induced lignification in red-fleshed loquat fruit by targeting the EjHB1-EjPRX12 module and EjXND1 is regulated by the dynamic of DNA methylation level in the promoter.http://www.sciencedirect.com/science/article/pii/S209012322400376XFruit qualityLignificationXND1Transcriptional regulationDNA methylation |
| spellingShingle | Zihao Liang Yanna Shi Yiqing Huang Jiao Lu Mengxue Zhang Xizhi Cao Ruoqian Hu Dongdong Li Wenbo Chen Changqing Zhu Di Wu Kunsong Chen XYLEM NAC DOMAIN 1 (EjXND1) relieves cold-induced lignification by negatively regulating the EjHB1-EjPRX12 module in loquat fruit Journal of Advanced Research Fruit quality Lignification XND1 Transcriptional regulation DNA methylation |
| title | XYLEM NAC DOMAIN 1 (EjXND1) relieves cold-induced lignification by negatively regulating the EjHB1-EjPRX12 module in loquat fruit |
| title_full | XYLEM NAC DOMAIN 1 (EjXND1) relieves cold-induced lignification by negatively regulating the EjHB1-EjPRX12 module in loquat fruit |
| title_fullStr | XYLEM NAC DOMAIN 1 (EjXND1) relieves cold-induced lignification by negatively regulating the EjHB1-EjPRX12 module in loquat fruit |
| title_full_unstemmed | XYLEM NAC DOMAIN 1 (EjXND1) relieves cold-induced lignification by negatively regulating the EjHB1-EjPRX12 module in loquat fruit |
| title_short | XYLEM NAC DOMAIN 1 (EjXND1) relieves cold-induced lignification by negatively regulating the EjHB1-EjPRX12 module in loquat fruit |
| title_sort | xylem nac domain 1 ejxnd1 relieves cold induced lignification by negatively regulating the ejhb1 ejprx12 module in loquat fruit |
| topic | Fruit quality Lignification XND1 Transcriptional regulation DNA methylation |
| url | http://www.sciencedirect.com/science/article/pii/S209012322400376X |
| work_keys_str_mv | AT zihaoliang xylemnacdomain1ejxnd1relievescoldinducedlignificationbynegativelyregulatingtheejhb1ejprx12moduleinloquatfruit AT yannashi xylemnacdomain1ejxnd1relievescoldinducedlignificationbynegativelyregulatingtheejhb1ejprx12moduleinloquatfruit AT yiqinghuang xylemnacdomain1ejxnd1relievescoldinducedlignificationbynegativelyregulatingtheejhb1ejprx12moduleinloquatfruit AT jiaolu xylemnacdomain1ejxnd1relievescoldinducedlignificationbynegativelyregulatingtheejhb1ejprx12moduleinloquatfruit AT mengxuezhang xylemnacdomain1ejxnd1relievescoldinducedlignificationbynegativelyregulatingtheejhb1ejprx12moduleinloquatfruit AT xizhicao xylemnacdomain1ejxnd1relievescoldinducedlignificationbynegativelyregulatingtheejhb1ejprx12moduleinloquatfruit AT ruoqianhu xylemnacdomain1ejxnd1relievescoldinducedlignificationbynegativelyregulatingtheejhb1ejprx12moduleinloquatfruit AT dongdongli xylemnacdomain1ejxnd1relievescoldinducedlignificationbynegativelyregulatingtheejhb1ejprx12moduleinloquatfruit AT wenbochen xylemnacdomain1ejxnd1relievescoldinducedlignificationbynegativelyregulatingtheejhb1ejprx12moduleinloquatfruit AT changqingzhu xylemnacdomain1ejxnd1relievescoldinducedlignificationbynegativelyregulatingtheejhb1ejprx12moduleinloquatfruit AT diwu xylemnacdomain1ejxnd1relievescoldinducedlignificationbynegativelyregulatingtheejhb1ejprx12moduleinloquatfruit AT kunsongchen xylemnacdomain1ejxnd1relievescoldinducedlignificationbynegativelyregulatingtheejhb1ejprx12moduleinloquatfruit |