Identification of the P450 gene family and genes involved in the formation of floral fragrance in Rosa chinensis(月季P450基因家族及其参与月季花香形成基因的鉴定)
In this study, evolutionary and expression analyses of the P450 gene family in Rosa chinensis were conducted using bioinformatics analysis methods, and the mechanism by which these genes are involved in the synthesis of rose fragrance was explored. A total of 448 P450 genes were identified in the ro...
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Zhejiang University Press
2025-02-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://doi.org/10.3785/j.issn.1008-9209.2024.10.091 |
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| author | 王君妍(WANG Junyan) 王峰(WANG Feng) 龚瑶(GONG Yao) 方慧仪(FANG Huiyi) 张亮生(ZHANG Liangsheng) |
| author_facet | 王君妍(WANG Junyan) 王峰(WANG Feng) 龚瑶(GONG Yao) 方慧仪(FANG Huiyi) 张亮生(ZHANG Liangsheng) |
| author_sort | 王君妍(WANG Junyan) |
| collection | DOAJ |
| description | In this study, evolutionary and expression analyses of the P450 gene family in Rosa chinensis were conducted using bioinformatics analysis methods, and the mechanism by which these genes are involved in the synthesis of rose fragrance was explored. A total of 448 P450 genes were identified in the rose genome. A phylogenetic tree of P450 genes with 4 849 P450 genes from 14 species, including Rosa chinensis, Rosa rugosa, Rosa wichurana, Pyrus bretschneideri,and Prunus persica was constructed and could be divided into 38 families. Among them, CYP71, CYP76, CYP81, CYP82, CYP706, CYP72, CYP714, CYP90, CYP86 and CYP94 underwent specific expansion within the Rosaceae. The results of transcriptome analysis showed that the expression patterns of rose P450 genes were tissue-specific. There were four genes highly expressed in petals. Most of the highly expressed P450 genes were related to the synthesis of terpenoids. Combined with metabolic data, 21 P450 genes potentially involved in rose fragrance synthesis were identified through weighted gene coexpression network analysis (WGCNA), such as RcHm_v2.0_Chr1g0351001_CYP86, RcHm_v2.0_Chr2g0115981_CYP71, RcHm_v2.0_Chr1g0359661_CYP82, etc. Most of these genes belonged to the significantly expanded CYP71 clan. This study reveals that multiple families expand in the Rosaceae and identified multiple P450 genes involve in fragrance synthesis, most of which are derived from the expanded CYP71 clan, indicating that P450 expansion may have promoted the diversity of rose fragrance.
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本研究运用生物信息学分析方法对月季P450基因家族进行进化与表达分析，并对其参与月季花香合成的机制进行探究。在月季基因组中，共鉴定出448个P450基因。通过对月季、玫瑰、光叶蔷薇、白梨、桃等14个物种的4 849个P450基因构建系统发育树，发现这些基因被划分为38个家族。其中，CYP71、CYP76、CYP81、CYP82、CYP706、CYP72、CYP714、CYP90、CYP86和CYP94在蔷薇科植物中发生特异性扩张。转录组分析结果显示，月季P450基因表达模式存在组织特异性，有4个基因在花瓣中高表达，且高表达的P450基因多与萜类物质合成相关。结合代谢数据，通过加权基因共表达网络分析鉴定到21个可能参与月季花香化合物合成的P450基因，如RcHm_v2.0_Chr1g0351001_CYP86、RcHm_v2.0_Chr2g0115981_CYP71、RcHm_v2.0_Chr1g0359661_CYP82等，其中大部分属于显著扩张的CYP71家族簇。本研究发现多个P450家族在蔷薇科植物中扩张，并鉴定到多个参与花香化合物合成的P450基因，且其大部分来自扩张的CYP71家族簇，表明P450基因扩张可能促进了月季花香多样性的形成。 |
| format | Article |
| id | doaj-art-3216172049ef458c96d31fa9101db7de |
| institution | Kabale University |
| issn | 2097-5155 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-3216172049ef458c96d31fa9101db7de2025-08-20T04:02:18ZengZhejiang University Press浙江大学学报. 农业与生命科学版2097-51552025-02-01511546610.3785/j.issn.1008-9209.2024.10.09110089209Identification of the P450 gene family and genes involved in the formation of floral fragrance in Rosa chinensis(月季P450基因家族及其参与月季花香形成基因的鉴定)王君妍(WANG Junyan)0https://orcid.org/0009-0003-3697-4624王峰(WANG Feng)1龚瑶(GONG Yao)2方慧仪(FANG Huiyi)3张亮生(ZHANG Liangsheng)4https://orcid.org/0000-0003-1919-3677Genomics and Genetic Engineering Laboratory of Ornamental Plants, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, Zhejiang, China(浙江大学农业与生物技术学院，观赏植物基因组与基因工程实验室，浙江 杭州 310058)Genomics and Genetic Engineering Laboratory of Ornamental Plants, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, Zhejiang, China(浙江大学农业与生物技术学院，观赏植物基因组与基因工程实验室，浙江 杭州 310058)Genomics and Genetic Engineering Laboratory of Ornamental Plants, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, Zhejiang, China(浙江大学农业与生物技术学院，观赏植物基因组与基因工程实验室，浙江 杭州 310058)Genomics and Genetic Engineering Laboratory of Ornamental Plants, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, Zhejiang, China(浙江大学农业与生物技术学院，观赏植物基因组与基因工程实验室，浙江 杭州 310058)Genomics and Genetic Engineering Laboratory of Ornamental Plants, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, Zhejiang, China(浙江大学农业与生物技术学院，观赏植物基因组与基因工程实验室，浙江 杭州 310058)In this study, evolutionary and expression analyses of the P450 gene family in Rosa chinensis were conducted using bioinformatics analysis methods, and the mechanism by which these genes are involved in the synthesis of rose fragrance was explored. A total of 448 P450 genes were identified in the rose genome. A phylogenetic tree of P450 genes with 4 849 P450 genes from 14 species, including Rosa chinensis, Rosa rugosa, Rosa wichurana, Pyrus bretschneideri,and Prunus persica was constructed and could be divided into 38 families. Among them, CYP71, CYP76, CYP81, CYP82, CYP706, CYP72, CYP714, CYP90, CYP86 and CYP94 underwent specific expansion within the Rosaceae. The results of transcriptome analysis showed that the expression patterns of rose P450 genes were tissue-specific. There were four genes highly expressed in petals. Most of the highly expressed P450 genes were related to the synthesis of terpenoids. Combined with metabolic data, 21 P450 genes potentially involved in rose fragrance synthesis were identified through weighted gene coexpression network analysis (WGCNA), such as RcHm_v2.0_Chr1g0351001_CYP86, RcHm_v2.0_Chr2g0115981_CYP71, RcHm_v2.0_Chr1g0359661_CYP82, etc. Most of these genes belonged to the significantly expanded CYP71 clan. This study reveals that multiple families expand in the Rosaceae and identified multiple P450 genes involve in fragrance synthesis, most of which are derived from the expanded CYP71 clan, indicating that P450 expansion may have promoted the diversity of rose fragrance. . 本研究运用生物信息学分析方法对月季P450基因家族进行进化与表达分析，并对其参与月季花香合成的机制进行探究。在月季基因组中，共鉴定出448个P450基因。通过对月季、玫瑰、光叶蔷薇、白梨、桃等14个物种的4 849个P450基因构建系统发育树，发现这些基因被划分为38个家族。其中，CYP71、CYP76、CYP81、CYP82、CYP706、CYP72、CYP714、CYP90、CYP86和CYP94在蔷薇科植物中发生特异性扩张。转录组分析结果显示，月季P450基因表达模式存在组织特异性，有4个基因在花瓣中高表达，且高表达的P450基因多与萜类物质合成相关。结合代谢数据，通过加权基因共表达网络分析鉴定到21个可能参与月季花香化合物合成的P450基因，如RcHm_v2.0_Chr1g0351001_CYP86、RcHm_v2.0_Chr2g0115981_CYP71、RcHm_v2.0_Chr1g0359661_CYP82等，其中大部分属于显著扩张的CYP71家族簇。本研究发现多个P450家族在蔷薇科植物中扩张，并鉴定到多个参与花香化合物合成的P450基因，且其大部分来自扩张的CYP71家族簇，表明P450基因扩张可能促进了月季花香多样性的形成。https://doi.org/10.3785/j.issn.1008-9209.2024.10.091p450基因家族(p450 gene family)蔷薇科(rosaceae)月季(rosa chinensis)花香(floral fragrance)萜类物质(terpenoids) |
| spellingShingle | 王君妍(WANG Junyan) 王峰(WANG Feng) 龚瑶(GONG Yao) 方慧仪(FANG Huiyi) 张亮生(ZHANG Liangsheng) Identification of the P450 gene family and genes involved in the formation of floral fragrance in Rosa chinensis(月季P450基因家族及其参与月季花香形成基因的鉴定) 浙江大学学报. 农业与生命科学版 p450基因家族(p450 gene family) 蔷薇科(rosaceae) 月季(rosa chinensis) 花香(floral fragrance) 萜类物质(terpenoids) |
| title | Identification of the P450 gene family and genes involved in the formation of floral fragrance in Rosa chinensis(月季P450基因家族及其参与月季花香形成基因的鉴定) |
| title_full | Identification of the P450 gene family and genes involved in the formation of floral fragrance in Rosa chinensis(月季P450基因家族及其参与月季花香形成基因的鉴定) |
| title_fullStr | Identification of the P450 gene family and genes involved in the formation of floral fragrance in Rosa chinensis(月季P450基因家族及其参与月季花香形成基因的鉴定) |
| title_full_unstemmed | Identification of the P450 gene family and genes involved in the formation of floral fragrance in Rosa chinensis(月季P450基因家族及其参与月季花香形成基因的鉴定) |
| title_short | Identification of the P450 gene family and genes involved in the formation of floral fragrance in Rosa chinensis(月季P450基因家族及其参与月季花香形成基因的鉴定) |
| title_sort | identification of the p450 gene family and genes involved in the formation of floral fragrance in rosa chinensis 月季p450基因家族及其参与月季花香形成基因的鉴定 |
| topic | p450基因家族(p450 gene family) 蔷薇科(rosaceae) 月季(rosa chinensis) 花香(floral fragrance) 萜类物质(terpenoids) |
| url | https://doi.org/10.3785/j.issn.1008-9209.2024.10.091 |
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