Revolutionizing Eco-Friendly Leather Production: A Freeze-Thaw and Liquid Fermentation Approach with Fungal Mycelium

The environmental impact and resource demands of traditional leather manufacturing have driven the search for sustainable alternatives. Fungal mycelium leather, recognised for its eco-friendly and renewable characteristics, has emerged as a promising option. This study established a cyclic freeze-th...

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Main Authors: Linxin Song, Yuxin Liu, Shijun Xiao, Xiaohui Yuan, Xuerong Han
Format: Article
Language:English
Published: MDPI AG 2025-04-01
Series:Journal of Fungi
Subjects:
Online Access:https://www.mdpi.com/2309-608X/11/4/326
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author Linxin Song
Yuxin Liu
Shijun Xiao
Xiaohui Yuan
Xuerong Han
author_facet Linxin Song
Yuxin Liu
Shijun Xiao
Xiaohui Yuan
Xuerong Han
author_sort Linxin Song
collection DOAJ
description The environmental impact and resource demands of traditional leather manufacturing have driven the search for sustainable alternatives. Fungal mycelium leather, recognised for its eco-friendly and renewable characteristics, has emerged as a promising option. This study established a cyclic freeze-thaw dehydration protocol for preparing mycelial leather using <i>Ganoderma</i> mycelium produced through liquid fermentation. By precisely controlling the fermentation parameters (pH 5.5, 150 rpm agitation, 28 °C), the liquid fermentation process ensures uniform mycelial growth, which is critical for subsequent structural enhancement during freeze-thaw cycles. After three freeze-thaw cycles were performed at −15 °C, uniformly distributed ice crystals facilitated effective water removal, achieving a minimum moisture content of 47.6%. The optimized freeze-thaw process produced membranes with a tensile strength of 6.22 MPa and elongation at break of 18.92%, demonstrating high mechanical performance. The freeze-thaw process was demonstrated to enhance structural integrity and mechanical properties while offering reduced energy consumption compared to conventional dehydration methods. This research provides a theoretical foundation and technical guidance for optimising fungal mycelium leather production and contributes to the development of sustainable bio-based materials for industrial applications.
format Article
id doaj-art-320dd59b0bb944e4b2815156cce82e1e
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issn 2309-608X
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publishDate 2025-04-01
publisher MDPI AG
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series Journal of Fungi
spelling doaj-art-320dd59b0bb944e4b2815156cce82e1e2025-08-20T02:28:31ZengMDPI AGJournal of Fungi2309-608X2025-04-0111432610.3390/jof11040326Revolutionizing Eco-Friendly Leather Production: A Freeze-Thaw and Liquid Fermentation Approach with Fungal MyceliumLinxin Song0Yuxin Liu1Shijun Xiao2Xiaohui Yuan3Xuerong Han4International Cooperation Research Center of China for New Germplasm Breeding of Edible Mushrooms, Jilin Agricultural University, Changchun 130118, ChinaInternational Cooperation Research Center of China for New Germplasm Breeding of Edible Mushrooms, Jilin Agricultural University, Changchun 130118, ChinaInternational Cooperation Research Center of China for New Germplasm Breeding of Edible Mushrooms, Jilin Agricultural University, Changchun 130118, ChinaInternational Cooperation Research Center of China for New Germplasm Breeding of Edible Mushrooms, Jilin Agricultural University, Changchun 130118, ChinaInternational Cooperation Research Center of China for New Germplasm Breeding of Edible Mushrooms, Jilin Agricultural University, Changchun 130118, ChinaThe environmental impact and resource demands of traditional leather manufacturing have driven the search for sustainable alternatives. Fungal mycelium leather, recognised for its eco-friendly and renewable characteristics, has emerged as a promising option. This study established a cyclic freeze-thaw dehydration protocol for preparing mycelial leather using <i>Ganoderma</i> mycelium produced through liquid fermentation. By precisely controlling the fermentation parameters (pH 5.5, 150 rpm agitation, 28 °C), the liquid fermentation process ensures uniform mycelial growth, which is critical for subsequent structural enhancement during freeze-thaw cycles. After three freeze-thaw cycles were performed at −15 °C, uniformly distributed ice crystals facilitated effective water removal, achieving a minimum moisture content of 47.6%. The optimized freeze-thaw process produced membranes with a tensile strength of 6.22 MPa and elongation at break of 18.92%, demonstrating high mechanical performance. The freeze-thaw process was demonstrated to enhance structural integrity and mechanical properties while offering reduced energy consumption compared to conventional dehydration methods. This research provides a theoretical foundation and technical guidance for optimising fungal mycelium leather production and contributes to the development of sustainable bio-based materials for industrial applications.https://www.mdpi.com/2309-608X/11/4/326freeze-thaw dehydrationice crystalsliquid fermentationfungal myceliummycelium leather
spellingShingle Linxin Song
Yuxin Liu
Shijun Xiao
Xiaohui Yuan
Xuerong Han
Revolutionizing Eco-Friendly Leather Production: A Freeze-Thaw and Liquid Fermentation Approach with Fungal Mycelium
Journal of Fungi
freeze-thaw dehydration
ice crystals
liquid fermentation
fungal mycelium
mycelium leather
title Revolutionizing Eco-Friendly Leather Production: A Freeze-Thaw and Liquid Fermentation Approach with Fungal Mycelium
title_full Revolutionizing Eco-Friendly Leather Production: A Freeze-Thaw and Liquid Fermentation Approach with Fungal Mycelium
title_fullStr Revolutionizing Eco-Friendly Leather Production: A Freeze-Thaw and Liquid Fermentation Approach with Fungal Mycelium
title_full_unstemmed Revolutionizing Eco-Friendly Leather Production: A Freeze-Thaw and Liquid Fermentation Approach with Fungal Mycelium
title_short Revolutionizing Eco-Friendly Leather Production: A Freeze-Thaw and Liquid Fermentation Approach with Fungal Mycelium
title_sort revolutionizing eco friendly leather production a freeze thaw and liquid fermentation approach with fungal mycelium
topic freeze-thaw dehydration
ice crystals
liquid fermentation
fungal mycelium
mycelium leather
url https://www.mdpi.com/2309-608X/11/4/326
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AT xiaohuiyuan revolutionizingecofriendlyleatherproductionafreezethawandliquidfermentationapproachwithfungalmycelium
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