Comprehensive Characterization of Mesenchymal Stem Cells from Human Placenta and Fetal Membrane and Their Response to Osteoactivin Stimulation
Mesenchymal stem cells (MSCs) are the most promising seed cells for cell therapy and can be isolated from various sources of human adult tissues such as bone marrow (BM-MSC) and adipose tissue. However, cells from these tissues must be obtained through invasive procedures. We, therefore, characteriz...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2012/658356 |
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| author | C. M. Raynaud M. Maleki R. Lis B. Ahmed I. Al-Azwani J. Malek F. F. Safadi A. Rafii |
| author_facet | C. M. Raynaud M. Maleki R. Lis B. Ahmed I. Al-Azwani J. Malek F. F. Safadi A. Rafii |
| author_sort | C. M. Raynaud |
| collection | DOAJ |
| description | Mesenchymal stem cells (MSCs) are the most promising seed cells for cell therapy and can be isolated from various sources of human adult tissues such as bone marrow (BM-MSC) and adipose tissue. However, cells from these tissues must be obtained through invasive procedures. We, therefore, characterized MSCs isolated from fresh placenta (Pl-MSC) and fetal membrane (Mb-MSC) through morphological and fluorescent-activated cell sorting (FACS). MSC frequency is higher in membrane than placenta (2.14% ± 0.65 versus 15.67% ± 0.29%). Pl/Mb-MSCs in vitro expansion potential was significantly higher than BM-MSCs. We demonstrated that one of the MSC-specific marker is sufficient for MSC isolation and that culture in specific media is the optimal way for selecting very homogenous MSC population. These MSCs could be differentiated into mesodermal cells expressing cell markers and cytologic staining consistent with mature osteoblasts and adipocytes. Transcriptomic analysis and cytokine arrays demonstrated broad similarity between placenta- and membrane-derived MSCs and only discrete differences with BM-MSCs with enrichment of networks involved in bone differentiation. Pl/Mb-MSCs displayed higher osteogenic differentiation potential than BM-MSC when their response to osteoactivin was evaluated. Fetal-tissue-derived mesenchymal cells may, therefore, be considered as a major source of MSCs to reach clinical scale banking in particular for bone regeneration. |
| format | Article |
| id | doaj-art-320ba1b77a5d409a97dcfa37f1eea90c |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-320ba1b77a5d409a97dcfa37f1eea90c2025-08-20T03:55:06ZengWileyStem Cells International1687-966X1687-96782012-01-01201210.1155/2012/658356658356Comprehensive Characterization of Mesenchymal Stem Cells from Human Placenta and Fetal Membrane and Their Response to Osteoactivin StimulationC. M. Raynaud0M. Maleki1R. Lis2B. Ahmed3I. Al-Azwani4J. Malek5F. F. Safadi6A. Rafii7Stem Cell and Microenvironment Laboratory, Weill Cornell Medical College in Qatar, Qatar Foundation, Doha 24144, QatarStem Cell and Microenvironment Laboratory, Weill Cornell Medical College in Qatar, Qatar Foundation, Doha 24144, QatarStem Cell and Microenvironment Laboratory, Weill Cornell Medical College in Qatar, Qatar Foundation, Doha 24144, QatarMaternal and Fetal Unit, Women Hospital, Hamad Medical Corporation, Doha 3050, QatarGenomic Core, Weill Cornell Medical College in Qatar, Qatar Foundation, Doha 24144, QatarGenomic Core, Weill Cornell Medical College in Qatar, Qatar Foundation, Doha 24144, QatarDepartments of Anatomy and Cell Biology, Orthopaedic Surgery and Sport Medicine, Temple University School of Medicine, Philadelphia, PA 19140, USAStem Cell and Microenvironment Laboratory, Weill Cornell Medical College in Qatar, Qatar Foundation, Doha 24144, QatarMesenchymal stem cells (MSCs) are the most promising seed cells for cell therapy and can be isolated from various sources of human adult tissues such as bone marrow (BM-MSC) and adipose tissue. However, cells from these tissues must be obtained through invasive procedures. We, therefore, characterized MSCs isolated from fresh placenta (Pl-MSC) and fetal membrane (Mb-MSC) through morphological and fluorescent-activated cell sorting (FACS). MSC frequency is higher in membrane than placenta (2.14% ± 0.65 versus 15.67% ± 0.29%). Pl/Mb-MSCs in vitro expansion potential was significantly higher than BM-MSCs. We demonstrated that one of the MSC-specific marker is sufficient for MSC isolation and that culture in specific media is the optimal way for selecting very homogenous MSC population. These MSCs could be differentiated into mesodermal cells expressing cell markers and cytologic staining consistent with mature osteoblasts and adipocytes. Transcriptomic analysis and cytokine arrays demonstrated broad similarity between placenta- and membrane-derived MSCs and only discrete differences with BM-MSCs with enrichment of networks involved in bone differentiation. Pl/Mb-MSCs displayed higher osteogenic differentiation potential than BM-MSC when their response to osteoactivin was evaluated. Fetal-tissue-derived mesenchymal cells may, therefore, be considered as a major source of MSCs to reach clinical scale banking in particular for bone regeneration.http://dx.doi.org/10.1155/2012/658356 |
| spellingShingle | C. M. Raynaud M. Maleki R. Lis B. Ahmed I. Al-Azwani J. Malek F. F. Safadi A. Rafii Comprehensive Characterization of Mesenchymal Stem Cells from Human Placenta and Fetal Membrane and Their Response to Osteoactivin Stimulation Stem Cells International |
| title | Comprehensive Characterization of Mesenchymal Stem Cells from Human Placenta and Fetal Membrane and Their Response to Osteoactivin Stimulation |
| title_full | Comprehensive Characterization of Mesenchymal Stem Cells from Human Placenta and Fetal Membrane and Their Response to Osteoactivin Stimulation |
| title_fullStr | Comprehensive Characterization of Mesenchymal Stem Cells from Human Placenta and Fetal Membrane and Their Response to Osteoactivin Stimulation |
| title_full_unstemmed | Comprehensive Characterization of Mesenchymal Stem Cells from Human Placenta and Fetal Membrane and Their Response to Osteoactivin Stimulation |
| title_short | Comprehensive Characterization of Mesenchymal Stem Cells from Human Placenta and Fetal Membrane and Their Response to Osteoactivin Stimulation |
| title_sort | comprehensive characterization of mesenchymal stem cells from human placenta and fetal membrane and their response to osteoactivin stimulation |
| url | http://dx.doi.org/10.1155/2012/658356 |
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