Brucellosis laboratory tests in Syria: what are their diagnostic efficacies in different clinical manifestations?
Introduction: Diagnosis of brucellosis in Syria is based on the presence of compatible symptoms in addition to positive agglutination results. This study investigated the potential of culture, ELISA and real-time PCR to support the diagnosis in different clinical manifestations of brucellosis. Met...
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The Journal of Infection in Developing Countries
2012-05-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/2453 |
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| author | Yara Alsayed Fawza Monem |
| author_facet | Yara Alsayed Fawza Monem |
| author_sort | Yara Alsayed |
| collection | DOAJ |
| description | Introduction: Diagnosis of brucellosis in Syria is based on the presence of compatible symptoms in addition to positive agglutination results. This study investigated the potential of culture, ELISA and real-time PCR to support the diagnosis in different clinical manifestations of brucellosis.
Methodology: Peripheral blood samples from 34 suspected brucellosis patients and 42 probable chronic or relapsed brucellosis patients were tested by agglutination tests, culture, ELISA and real-time PCR.
Results: Among 34 samples collected from suspected cases, 18/34 (53%) were agglutination tests positive, 12/34 (35%) were culture positive, 12/34 (35%) were Brucella IgG positive, and 10/34 (29%) were real-time PCR positive. Three out of 34 patients were positive by real-time PCR but not by agglutination tests or culture. Among 42 samples obtained from probable chronic or relapsed patients, 27/42 (64%) were agglutination tests positive, 26/42 (62%) were Brucella IgG positive, 4/42 (10%) were culture positive, and 1/42 (2%) was real-time PCR positive.
Conclusion: To rule in or rule out the diagnosis of brucellosis, a combination of several tests should be applied. Agglutination tests should be performed first considering their high sensitivity. If the agglutination test is negative, real-time PCR, and/or ELISA, and/or culture should be performed. When relapse or chronic brucellosis are suspected, agglutination tests and/or ELISA are recommended.
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| format | Article |
| id | doaj-art-320950a54b00403588e02b339b5fcd0c |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2012-05-01 |
| publisher | The Journal of Infection in Developing Countries |
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| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-320950a54b00403588e02b339b5fcd0c2025-08-20T02:27:18ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802012-05-0160610.3855/jidc.2453Brucellosis laboratory tests in Syria: what are their diagnostic efficacies in different clinical manifestations?Yara Alsayed0Fawza Monem1Faculty of Pharmacy, Damascus University, Damascus, SyriaAL-Assad Hospital, Damascus University, P.O. Box 10769, Damascus, SyriaIntroduction: Diagnosis of brucellosis in Syria is based on the presence of compatible symptoms in addition to positive agglutination results. This study investigated the potential of culture, ELISA and real-time PCR to support the diagnosis in different clinical manifestations of brucellosis. Methodology: Peripheral blood samples from 34 suspected brucellosis patients and 42 probable chronic or relapsed brucellosis patients were tested by agglutination tests, culture, ELISA and real-time PCR. Results: Among 34 samples collected from suspected cases, 18/34 (53%) were agglutination tests positive, 12/34 (35%) were culture positive, 12/34 (35%) were Brucella IgG positive, and 10/34 (29%) were real-time PCR positive. Three out of 34 patients were positive by real-time PCR but not by agglutination tests or culture. Among 42 samples obtained from probable chronic or relapsed patients, 27/42 (64%) were agglutination tests positive, 26/42 (62%) were Brucella IgG positive, 4/42 (10%) were culture positive, and 1/42 (2%) was real-time PCR positive. Conclusion: To rule in or rule out the diagnosis of brucellosis, a combination of several tests should be applied. Agglutination tests should be performed first considering their high sensitivity. If the agglutination test is negative, real-time PCR, and/or ELISA, and/or culture should be performed. When relapse or chronic brucellosis are suspected, agglutination tests and/or ELISA are recommended. https://jidc.org/index.php/journal/article/view/2453brucellosisagglutination testsculturereal-time PCRELISASyria |
| spellingShingle | Yara Alsayed Fawza Monem Brucellosis laboratory tests in Syria: what are their diagnostic efficacies in different clinical manifestations? Journal of Infection in Developing Countries brucellosis agglutination tests culture real-time PCR ELISA Syria |
| title | Brucellosis laboratory tests in Syria: what are their diagnostic efficacies in different clinical manifestations? |
| title_full | Brucellosis laboratory tests in Syria: what are their diagnostic efficacies in different clinical manifestations? |
| title_fullStr | Brucellosis laboratory tests in Syria: what are their diagnostic efficacies in different clinical manifestations? |
| title_full_unstemmed | Brucellosis laboratory tests in Syria: what are their diagnostic efficacies in different clinical manifestations? |
| title_short | Brucellosis laboratory tests in Syria: what are their diagnostic efficacies in different clinical manifestations? |
| title_sort | brucellosis laboratory tests in syria what are their diagnostic efficacies in different clinical manifestations |
| topic | brucellosis agglutination tests culture real-time PCR ELISA Syria |
| url | https://jidc.org/index.php/journal/article/view/2453 |
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