A Bioelectric Active Hydrogel Sensor for Trace Detection of Heavy Metal Ions in Livestock and Poultry Farm Wastewater
Heavy metal contamination in livestock and poultry farm wastewater poses significant risks to both the environment and human health, so it is critical to accurately and rapidly quantify heavy metal ion concentrations in water. This research develops a bioelectric active hydrogel sensor for detecting...
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| Format: | Article |
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MDPI AG
2025-05-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/15/6/341 |
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| author | Heng-Chi Liu Jia-Xin Du Jie Wang Junying Liu Luyu Yang Yang-Chun Yong |
| author_facet | Heng-Chi Liu Jia-Xin Du Jie Wang Junying Liu Luyu Yang Yang-Chun Yong |
| author_sort | Heng-Chi Liu |
| collection | DOAJ |
| description | Heavy metal contamination in livestock and poultry farm wastewater poses significant risks to both the environment and human health, so it is critical to accurately and rapidly quantify heavy metal ion concentrations in water. This research develops a bioelectric active hydrogel sensor for detecting heavy metal ions in livestock wastewater. The sensor integrates microbial surface display technology with graphene hydrogel, displaying glucose oxidase (GOx) on the surface of yeast cells, and covalently incorporating it into the graphene hydrogel through the bio-reduction activity of metal-reducing bacteria, enhancing its electrochemical performance. The sensor demonstrates excellent sensitivity and stability in detecting Cu<sup>2+</sup>, with a detection limit for Cu<sup>2+</sup> of 17.0 µM. This sensor is also applicable for detecting Zn<sup>2+</sup> in wastewater. When various heavy metal ions coexist in the solution, they exert a more pronounced inhibitory effect on enzyme activity. Consequently, the sensor can be employed to assess the overall heavy metal content in water samples. In the detection of Cu<sup>2+</sup> in real livestock and poultry wastewater, the recovery rate of the graphene hydrogel electrode ranged from 88% to 106.5%, indicating that the sensor holds significant potential for application in actual sample analysis. |
| format | Article |
| id | doaj-art-31fcb4d85fcd495da955a6c5e02affca |
| institution | OA Journals |
| issn | 2079-6374 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj-art-31fcb4d85fcd495da955a6c5e02affca2025-08-20T02:24:37ZengMDPI AGBiosensors2079-63742025-05-0115634110.3390/bios15060341A Bioelectric Active Hydrogel Sensor for Trace Detection of Heavy Metal Ions in Livestock and Poultry Farm WastewaterHeng-Chi Liu0Jia-Xin Du1Jie Wang2Junying Liu3Luyu Yang4Yang-Chun Yong5Institute for Energy Research, School of Environment and Safety Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, ChinaInstitute for Energy Research, School of Environment and Safety Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, ChinaInstitute for Energy Research, School of Environment and Safety Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, ChinaInstitute for Energy Research, School of Environment and Safety Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, ChinaInstitute for Energy Research, School of Environment and Safety Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, ChinaInstitute for Energy Research, School of Environment and Safety Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, ChinaHeavy metal contamination in livestock and poultry farm wastewater poses significant risks to both the environment and human health, so it is critical to accurately and rapidly quantify heavy metal ion concentrations in water. This research develops a bioelectric active hydrogel sensor for detecting heavy metal ions in livestock wastewater. The sensor integrates microbial surface display technology with graphene hydrogel, displaying glucose oxidase (GOx) on the surface of yeast cells, and covalently incorporating it into the graphene hydrogel through the bio-reduction activity of metal-reducing bacteria, enhancing its electrochemical performance. The sensor demonstrates excellent sensitivity and stability in detecting Cu<sup>2+</sup>, with a detection limit for Cu<sup>2+</sup> of 17.0 µM. This sensor is also applicable for detecting Zn<sup>2+</sup> in wastewater. When various heavy metal ions coexist in the solution, they exert a more pronounced inhibitory effect on enzyme activity. Consequently, the sensor can be employed to assess the overall heavy metal content in water samples. In the detection of Cu<sup>2+</sup> in real livestock and poultry wastewater, the recovery rate of the graphene hydrogel electrode ranged from 88% to 106.5%, indicating that the sensor holds significant potential for application in actual sample analysis.https://www.mdpi.com/2079-6374/15/6/341livestock and poultry wastewatermicrobial surface displaygraphene hydrogelheavy metalsensors |
| spellingShingle | Heng-Chi Liu Jia-Xin Du Jie Wang Junying Liu Luyu Yang Yang-Chun Yong A Bioelectric Active Hydrogel Sensor for Trace Detection of Heavy Metal Ions in Livestock and Poultry Farm Wastewater Biosensors livestock and poultry wastewater microbial surface display graphene hydrogel heavy metal sensors |
| title | A Bioelectric Active Hydrogel Sensor for Trace Detection of Heavy Metal Ions in Livestock and Poultry Farm Wastewater |
| title_full | A Bioelectric Active Hydrogel Sensor for Trace Detection of Heavy Metal Ions in Livestock and Poultry Farm Wastewater |
| title_fullStr | A Bioelectric Active Hydrogel Sensor for Trace Detection of Heavy Metal Ions in Livestock and Poultry Farm Wastewater |
| title_full_unstemmed | A Bioelectric Active Hydrogel Sensor for Trace Detection of Heavy Metal Ions in Livestock and Poultry Farm Wastewater |
| title_short | A Bioelectric Active Hydrogel Sensor for Trace Detection of Heavy Metal Ions in Livestock and Poultry Farm Wastewater |
| title_sort | bioelectric active hydrogel sensor for trace detection of heavy metal ions in livestock and poultry farm wastewater |
| topic | livestock and poultry wastewater microbial surface display graphene hydrogel heavy metal sensors |
| url | https://www.mdpi.com/2079-6374/15/6/341 |
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