Protocol to investigate bivalent histone modification dynamics via chromatin immunoprecipitation followed by re-chromatin immunoprecipitation
Summary: Epigenetic modifications play key roles in regulating transcription by altering chromatin structure and accessibility in response to internal cues or external stimuli. Here, we present a protocol for assessing bivalent histone modification changes through chromatin immunoprecipitation (ChIP...
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| Language: | English |
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Elsevier
2025-06-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725002102 |
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| author | Zhengyao Shao Hong Qiao |
| author_facet | Zhengyao Shao Hong Qiao |
| author_sort | Zhengyao Shao |
| collection | DOAJ |
| description | Summary: Epigenetic modifications play key roles in regulating transcription by altering chromatin structure and accessibility in response to internal cues or external stimuli. Here, we present a protocol for assessing bivalent histone modification changes through chromatin immunoprecipitation (ChIP)-reChIP assay in response to warm ambient temperature in Arabidopsis. We describe steps to perform transient warm ambient temperature treatment on Arabidopsis seedlings. We then detailed procedures for ChIP-reChIP, which can be applied to investigate various DNA-protein interaction dynamics in different experimental contexts.For complete details on the use and execution of this protocol, please refer to Shao et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-31f975f2dd2c4c97bd92fd53ab973a14 |
| institution | DOAJ |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-31f975f2dd2c4c97bd92fd53ab973a142025-08-20T03:11:06ZengElsevierSTAR Protocols2666-16672025-06-016210380410.1016/j.xpro.2025.103804Protocol to investigate bivalent histone modification dynamics via chromatin immunoprecipitation followed by re-chromatin immunoprecipitationZhengyao Shao0Hong Qiao1Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, TX 78712, USA; Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX 78712, USA; Corresponding authorInstitute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, TX 78712, USA; Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX 78712, USA; Corresponding authorSummary: Epigenetic modifications play key roles in regulating transcription by altering chromatin structure and accessibility in response to internal cues or external stimuli. Here, we present a protocol for assessing bivalent histone modification changes through chromatin immunoprecipitation (ChIP)-reChIP assay in response to warm ambient temperature in Arabidopsis. We describe steps to perform transient warm ambient temperature treatment on Arabidopsis seedlings. We then detailed procedures for ChIP-reChIP, which can be applied to investigate various DNA-protein interaction dynamics in different experimental contexts.For complete details on the use and execution of this protocol, please refer to Shao et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166725002102Plant sciencesMolecular BiologyChromatin immunoprecipitationChIP |
| spellingShingle | Zhengyao Shao Hong Qiao Protocol to investigate bivalent histone modification dynamics via chromatin immunoprecipitation followed by re-chromatin immunoprecipitation STAR Protocols Plant sciences Molecular Biology Chromatin immunoprecipitation ChIP |
| title | Protocol to investigate bivalent histone modification dynamics via chromatin immunoprecipitation followed by re-chromatin immunoprecipitation |
| title_full | Protocol to investigate bivalent histone modification dynamics via chromatin immunoprecipitation followed by re-chromatin immunoprecipitation |
| title_fullStr | Protocol to investigate bivalent histone modification dynamics via chromatin immunoprecipitation followed by re-chromatin immunoprecipitation |
| title_full_unstemmed | Protocol to investigate bivalent histone modification dynamics via chromatin immunoprecipitation followed by re-chromatin immunoprecipitation |
| title_short | Protocol to investigate bivalent histone modification dynamics via chromatin immunoprecipitation followed by re-chromatin immunoprecipitation |
| title_sort | protocol to investigate bivalent histone modification dynamics via chromatin immunoprecipitation followed by re chromatin immunoprecipitation |
| topic | Plant sciences Molecular Biology Chromatin immunoprecipitation ChIP |
| url | http://www.sciencedirect.com/science/article/pii/S2666166725002102 |
| work_keys_str_mv | AT zhengyaoshao protocoltoinvestigatebivalenthistonemodificationdynamicsviachromatinimmunoprecipitationfollowedbyrechromatinimmunoprecipitation AT hongqiao protocoltoinvestigatebivalenthistonemodificationdynamicsviachromatinimmunoprecipitationfollowedbyrechromatinimmunoprecipitation |