Protocol to investigate bivalent histone modification dynamics via chromatin immunoprecipitation followed by re-chromatin immunoprecipitation
Summary: Epigenetic modifications play key roles in regulating transcription by altering chromatin structure and accessibility in response to internal cues or external stimuli. Here, we present a protocol for assessing bivalent histone modification changes through chromatin immunoprecipitation (ChIP...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-06-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725002102 |
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| Summary: | Summary: Epigenetic modifications play key roles in regulating transcription by altering chromatin structure and accessibility in response to internal cues or external stimuli. Here, we present a protocol for assessing bivalent histone modification changes through chromatin immunoprecipitation (ChIP)-reChIP assay in response to warm ambient temperature in Arabidopsis. We describe steps to perform transient warm ambient temperature treatment on Arabidopsis seedlings. We then detailed procedures for ChIP-reChIP, which can be applied to investigate various DNA-protein interaction dynamics in different experimental contexts.For complete details on the use and execution of this protocol, please refer to Shao et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
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| ISSN: | 2666-1667 |