Isolation and Characterization of a Lytic Phage PaTJ Against <i>Pseudomonas aeruginosa</i>
<i>Pseudomonas aeruginosa</i> is a major global threat to human health, and phage therapy has emerged as a promising strategy for treating infections caused by multidrug-resistant pathogens. In this study, we isolated and characterized a <i>Pseudomonas</i> lytic phage, PaTJ,...
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2024-11-01
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| author | Jiayu Gu Xinqiao Zhang Tianlang Liu Yunxue Guo |
| author_facet | Jiayu Gu Xinqiao Zhang Tianlang Liu Yunxue Guo |
| author_sort | Jiayu Gu |
| collection | DOAJ |
| description | <i>Pseudomonas aeruginosa</i> is a major global threat to human health, and phage therapy has emerged as a promising strategy for treating infections caused by multidrug-resistant pathogens. In this study, we isolated and characterized a <i>Pseudomonas</i> lytic phage, PaTJ, from wastewater. PaTJ belongs to the phage family <i>Mesyanzhinovviridae</i>, and is featured by short latency (30 min) and large burst size (10<sup>3</sup> PFU per infected cell). Our investigation revealed that PaTJ utilizes the type IV Pili (T4P) as a receptor. Transcriptome analysis of PaTJ infected host at latent stage showed distinct expression patterns of PaTJ encoding genes involved in replication and structure assembly, without expression of the majority of toxic accessory genes responsible for phage release. In addition, host bacteria exhibited specific induction of host metabolism-related genes in response to the PaTJ’s infection. Furthermore, our findings demonstrated the PaTJ’s potential in degrading biofilms. This work sheds light on the multifaceted impact of this lytic phage PaTJ on <i>P. aeruginosa</i>, presenting potential applications in both gene expression modulation and biofilm management. |
| format | Article |
| id | doaj-art-31f5e2d2efbf4b4aac2e41a09bb8ddfb |
| institution | DOAJ |
| issn | 1999-4915 |
| language | English |
| publishDate | 2024-11-01 |
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| series | Viruses |
| spelling | doaj-art-31f5e2d2efbf4b4aac2e41a09bb8ddfb2025-08-20T02:43:46ZengMDPI AGViruses1999-49152024-11-011612181610.3390/v16121816Isolation and Characterization of a Lytic Phage PaTJ Against <i>Pseudomonas aeruginosa</i>Jiayu Gu0Xinqiao Zhang1Tianlang Liu2Yunxue Guo3Key Laboratory of Tropical Marine Bio-Resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510000, ChinaFrontiers Science Center for Synthetic Biology (Ministry of Education), Tianjin University, Tianjin 300000, ChinaKey Laboratory of Tropical Marine Bio-Resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510000, ChinaKey Laboratory of Tropical Marine Bio-Resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510000, China<i>Pseudomonas aeruginosa</i> is a major global threat to human health, and phage therapy has emerged as a promising strategy for treating infections caused by multidrug-resistant pathogens. In this study, we isolated and characterized a <i>Pseudomonas</i> lytic phage, PaTJ, from wastewater. PaTJ belongs to the phage family <i>Mesyanzhinovviridae</i>, and is featured by short latency (30 min) and large burst size (10<sup>3</sup> PFU per infected cell). Our investigation revealed that PaTJ utilizes the type IV Pili (T4P) as a receptor. Transcriptome analysis of PaTJ infected host at latent stage showed distinct expression patterns of PaTJ encoding genes involved in replication and structure assembly, without expression of the majority of toxic accessory genes responsible for phage release. In addition, host bacteria exhibited specific induction of host metabolism-related genes in response to the PaTJ’s infection. Furthermore, our findings demonstrated the PaTJ’s potential in degrading biofilms. This work sheds light on the multifaceted impact of this lytic phage PaTJ on <i>P. aeruginosa</i>, presenting potential applications in both gene expression modulation and biofilm management.https://www.mdpi.com/1999-4915/16/12/1816<i>Pseudomonas aeruginosa</i>lytic phagemetabolismtype IV pili |
| spellingShingle | Jiayu Gu Xinqiao Zhang Tianlang Liu Yunxue Guo Isolation and Characterization of a Lytic Phage PaTJ Against <i>Pseudomonas aeruginosa</i> Viruses <i>Pseudomonas aeruginosa</i> lytic phage metabolism type IV pili |
| title | Isolation and Characterization of a Lytic Phage PaTJ Against <i>Pseudomonas aeruginosa</i> |
| title_full | Isolation and Characterization of a Lytic Phage PaTJ Against <i>Pseudomonas aeruginosa</i> |
| title_fullStr | Isolation and Characterization of a Lytic Phage PaTJ Against <i>Pseudomonas aeruginosa</i> |
| title_full_unstemmed | Isolation and Characterization of a Lytic Phage PaTJ Against <i>Pseudomonas aeruginosa</i> |
| title_short | Isolation and Characterization of a Lytic Phage PaTJ Against <i>Pseudomonas aeruginosa</i> |
| title_sort | isolation and characterization of a lytic phage patj against i pseudomonas aeruginosa i |
| topic | <i>Pseudomonas aeruginosa</i> lytic phage metabolism type IV pili |
| url | https://www.mdpi.com/1999-4915/16/12/1816 |
| work_keys_str_mv | AT jiayugu isolationandcharacterizationofalyticphagepatjagainstipseudomonasaeruginosai AT xinqiaozhang isolationandcharacterizationofalyticphagepatjagainstipseudomonasaeruginosai AT tianlangliu isolationandcharacterizationofalyticphagepatjagainstipseudomonasaeruginosai AT yunxueguo isolationandcharacterizationofalyticphagepatjagainstipseudomonasaeruginosai |