Effects of FK506 on Hippocampal CA1 Cells Following Transient Global Ischemia/Reperfusion in Wistar Rat

Transient global cerebral ischemia causes loss of pyramidal cells in CA1 region of hippocampus. In this study, we investigated the neurotrophic effect of the immunosuppressant agent FK506 in rat after global cerebral ischemia. Both common carotid arteries were occluded for 20 minutes followed by rep...

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Main Authors: Zahra-Nadia Sharifi, Farid Abolhassani, Mohammad Reza Zarrindast, Shabnam Movassaghi, Nasrin Rahimian, Gholamreza Hassanzadeh
Format: Article
Language:English
Published: Wiley 2012-01-01
Series:Stroke Research and Treatment
Online Access:http://dx.doi.org/10.1155/2012/809417
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author Zahra-Nadia Sharifi
Farid Abolhassani
Mohammad Reza Zarrindast
Shabnam Movassaghi
Nasrin Rahimian
Gholamreza Hassanzadeh
author_facet Zahra-Nadia Sharifi
Farid Abolhassani
Mohammad Reza Zarrindast
Shabnam Movassaghi
Nasrin Rahimian
Gholamreza Hassanzadeh
author_sort Zahra-Nadia Sharifi
collection DOAJ
description Transient global cerebral ischemia causes loss of pyramidal cells in CA1 region of hippocampus. In this study, we investigated the neurotrophic effect of the immunosuppressant agent FK506 in rat after global cerebral ischemia. Both common carotid arteries were occluded for 20 minutes followed by reperfusion. In experimental group 1, FK506 (6 mg/kg) was given as a single dose exactly at the time of reperfusion. In the second group, FK506 was administered at the beginning of reperfusion, followed by its administration intraperitoneally (IP) 6, 24, 48, and 72 hours after reperfusion. FK506 failed to show neurotrophic effects on CA1 region when applied as a single dose of 6 mg/kg. The cell number and size of the CA1 pyramidal cells were increased, also the number of cell death decreased in this region when FK506 was administrated 48 h after reperfusion. This work supports the possible use of FK506 in treatment of ischemic brain damage.
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publisher Wiley
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series Stroke Research and Treatment
spelling doaj-art-31f4cc526eaf4f18b8a34099e7c433892025-08-20T02:07:40ZengWileyStroke Research and Treatment2090-81052042-00562012-01-01201210.1155/2012/809417809417Effects of FK506 on Hippocampal CA1 Cells Following Transient Global Ischemia/Reperfusion in Wistar RatZahra-Nadia Sharifi0Farid Abolhassani1Mohammad Reza Zarrindast2Shabnam Movassaghi3Nasrin Rahimian4Gholamreza Hassanzadeh5Institute for Cognitive Science Studies, Pezeshkpour Alley, Vali-e-Asr Street, 15948-34111 Tehran, IranDepartment of Anatomy, School of Medicine, Tehran University of Medical Sciences, Enghelab Street, 14176-13151 Tehran, IranDepartment of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Enghelab Street, 14176-13151 Tehran, IranDepartment of Anatomy, School of Medicine, Tehran Medical Branch, Islamic Azad University, Shariati Street, Zargandeh Street, 19168 Tehran, IranDepartment of Neurology, Imam Khomeini Hospital, Tehran University of Medical Sciences, Dr. Gharib Street, 14197-31357 Tehran, IranDepartment of Anatomy, School of Medicine, Tehran University of Medical Sciences, Enghelab Street, 14176-13151 Tehran, IranTransient global cerebral ischemia causes loss of pyramidal cells in CA1 region of hippocampus. In this study, we investigated the neurotrophic effect of the immunosuppressant agent FK506 in rat after global cerebral ischemia. Both common carotid arteries were occluded for 20 minutes followed by reperfusion. In experimental group 1, FK506 (6 mg/kg) was given as a single dose exactly at the time of reperfusion. In the second group, FK506 was administered at the beginning of reperfusion, followed by its administration intraperitoneally (IP) 6, 24, 48, and 72 hours after reperfusion. FK506 failed to show neurotrophic effects on CA1 region when applied as a single dose of 6 mg/kg. The cell number and size of the CA1 pyramidal cells were increased, also the number of cell death decreased in this region when FK506 was administrated 48 h after reperfusion. This work supports the possible use of FK506 in treatment of ischemic brain damage.http://dx.doi.org/10.1155/2012/809417
spellingShingle Zahra-Nadia Sharifi
Farid Abolhassani
Mohammad Reza Zarrindast
Shabnam Movassaghi
Nasrin Rahimian
Gholamreza Hassanzadeh
Effects of FK506 on Hippocampal CA1 Cells Following Transient Global Ischemia/Reperfusion in Wistar Rat
Stroke Research and Treatment
title Effects of FK506 on Hippocampal CA1 Cells Following Transient Global Ischemia/Reperfusion in Wistar Rat
title_full Effects of FK506 on Hippocampal CA1 Cells Following Transient Global Ischemia/Reperfusion in Wistar Rat
title_fullStr Effects of FK506 on Hippocampal CA1 Cells Following Transient Global Ischemia/Reperfusion in Wistar Rat
title_full_unstemmed Effects of FK506 on Hippocampal CA1 Cells Following Transient Global Ischemia/Reperfusion in Wistar Rat
title_short Effects of FK506 on Hippocampal CA1 Cells Following Transient Global Ischemia/Reperfusion in Wistar Rat
title_sort effects of fk506 on hippocampal ca1 cells following transient global ischemia reperfusion in wistar rat
url http://dx.doi.org/10.1155/2012/809417
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