A General Strategy to Fine‐Tune Group 14 Rhodamines for Ultrahigh Signal‐to‐Noise Ratio Labeling In Vivo by Nano‐Aggregation
ABSTRACT Ultrahigh signal‐to‐noise ratio (SNR) labeling enables precise visualization of biological structures in vivo. We boosted fluorogenicity in group‐14‐rhodamines by comprehensively manipulating their dynamics in physical (aggregate/monomer, KA/M) and chemical (closed/open spirolactone, KC/O)...
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Wiley
2025-08-01
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| Online Access: | https://doi.org/10.1002/agt2.70077 |
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| author | Ning Wang Ting Wang Mengting Fan Chen Li Yue Tian Xiaoyan Cui |
| author_facet | Ning Wang Ting Wang Mengting Fan Chen Li Yue Tian Xiaoyan Cui |
| author_sort | Ning Wang |
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| description | ABSTRACT Ultrahigh signal‐to‐noise ratio (SNR) labeling enables precise visualization of biological structures in vivo. We boosted fluorogenicity in group‐14‐rhodamines by comprehensively manipulating their dynamics in physical (aggregate/monomer, KA/M) and chemical (closed/open spirolactone, KC/O) states. Fluorogenic rhodamines were designed by group 14 (C, Si, Ge) substituted bridging regions in xanthene with tuned dialkylation. We quantified the impact of alkylation with the hydrophobicity (logP) over a wide range and confirmed that SNR can be sharply improved, owing to the promoted nano‐aggregation (KA/M) with high logP. Integrating KA/M with KC/O mechanisms, unparalleled fluorogenicity was observed in group‐14‐rhodamines: HaloTag probe with dipentylsilyl exhibits remarkable fluorogenicity (>2000) in vitro, enabling no‐wash and multicolor super‐resolution stimulated emission depletion imaging of high SNR (>300) in vivo. Overexpression of αvβ3 was sensitively tracked in vivo by RGDyK‐based fluorogenic SiR probe through tuned KA/M. Our proposed strategy has significantly promoted the fluorogenicity of group 14 rhodamines as a general mechanism. |
| format | Article |
| id | doaj-art-31e0c79849604e8f8c7e7abbd6cafff0 |
| institution | Kabale University |
| issn | 2692-4560 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Wiley |
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| spelling | doaj-art-31e0c79849604e8f8c7e7abbd6cafff02025-08-21T14:18:21ZengWileyAggregate2692-45602025-08-0168n/an/a10.1002/agt2.70077A General Strategy to Fine‐Tune Group 14 Rhodamines for Ultrahigh Signal‐to‐Noise Ratio Labeling In Vivo by Nano‐AggregationNing Wang0Ting Wang1Mengting Fan2Chen Li3Yue Tian4Xiaoyan Cui5Department of Chemistry School of Chemistry and Molecular Engineering East China Normal University Shanghai ChinaShanghai Skin Disease Hospital School of Medicine Tongji University Shanghai ChinaDepartment of Chemistry School of Chemistry and Molecular Engineering East China Normal University Shanghai ChinaDepartment of Chemistry School of Chemistry and Molecular Engineering East China Normal University Shanghai ChinaDepartment of Chemistry School of Chemistry and Molecular Engineering East China Normal University Shanghai ChinaDepartment of Chemistry School of Chemistry and Molecular Engineering East China Normal University Shanghai ChinaABSTRACT Ultrahigh signal‐to‐noise ratio (SNR) labeling enables precise visualization of biological structures in vivo. We boosted fluorogenicity in group‐14‐rhodamines by comprehensively manipulating their dynamics in physical (aggregate/monomer, KA/M) and chemical (closed/open spirolactone, KC/O) states. Fluorogenic rhodamines were designed by group 14 (C, Si, Ge) substituted bridging regions in xanthene with tuned dialkylation. We quantified the impact of alkylation with the hydrophobicity (logP) over a wide range and confirmed that SNR can be sharply improved, owing to the promoted nano‐aggregation (KA/M) with high logP. Integrating KA/M with KC/O mechanisms, unparalleled fluorogenicity was observed in group‐14‐rhodamines: HaloTag probe with dipentylsilyl exhibits remarkable fluorogenicity (>2000) in vitro, enabling no‐wash and multicolor super‐resolution stimulated emission depletion imaging of high SNR (>300) in vivo. Overexpression of αvβ3 was sensitively tracked in vivo by RGDyK‐based fluorogenic SiR probe through tuned KA/M. Our proposed strategy has significantly promoted the fluorogenicity of group 14 rhodamines as a general mechanism.https://doi.org/10.1002/agt2.70077fluorescence imagingnano‐aggregatessignal‐to‐noise ratiosubstituted rhodaminesuper‐resolution microscopy |
| spellingShingle | Ning Wang Ting Wang Mengting Fan Chen Li Yue Tian Xiaoyan Cui A General Strategy to Fine‐Tune Group 14 Rhodamines for Ultrahigh Signal‐to‐Noise Ratio Labeling In Vivo by Nano‐Aggregation Aggregate fluorescence imaging nano‐aggregates signal‐to‐noise ratio substituted rhodamine super‐resolution microscopy |
| title | A General Strategy to Fine‐Tune Group 14 Rhodamines for Ultrahigh Signal‐to‐Noise Ratio Labeling In Vivo by Nano‐Aggregation |
| title_full | A General Strategy to Fine‐Tune Group 14 Rhodamines for Ultrahigh Signal‐to‐Noise Ratio Labeling In Vivo by Nano‐Aggregation |
| title_fullStr | A General Strategy to Fine‐Tune Group 14 Rhodamines for Ultrahigh Signal‐to‐Noise Ratio Labeling In Vivo by Nano‐Aggregation |
| title_full_unstemmed | A General Strategy to Fine‐Tune Group 14 Rhodamines for Ultrahigh Signal‐to‐Noise Ratio Labeling In Vivo by Nano‐Aggregation |
| title_short | A General Strategy to Fine‐Tune Group 14 Rhodamines for Ultrahigh Signal‐to‐Noise Ratio Labeling In Vivo by Nano‐Aggregation |
| title_sort | general strategy to fine tune group 14 rhodamines for ultrahigh signal to noise ratio labeling in vivo by nano aggregation |
| topic | fluorescence imaging nano‐aggregates signal‐to‐noise ratio substituted rhodamine super‐resolution microscopy |
| url | https://doi.org/10.1002/agt2.70077 |
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