Cellulases production on paper and sawdust using native Trichoderma asperellum
Microbial cellulases are industrially used enzymes that catalyze the cleavage of the glycosidic bonds of cellulose. This hydrolysis yields sugars that can be used in processes such as bioethanol production. These enzymes are mainly produced by fungi belonging to the genus Trichoderma via submerge...
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| Language: | English |
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Pontificia Universidad Javeriana
2018-11-01
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| Series: | Universitas Scientiarum |
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| Online Access: | https://revistas.javeriana.edu.co/index.php/scientarium/article/view/19652 |
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| author | Y-Maritza Zapata, Angelica Galviz-Quezada, Víctor-Manuel Osorio-Echeverri |
| author_facet | Y-Maritza Zapata, Angelica Galviz-Quezada, Víctor-Manuel Osorio-Echeverri |
| author_sort | Y-Maritza Zapata, Angelica Galviz-Quezada, Víctor-Manuel Osorio-Echeverri |
| collection | DOAJ |
| description | Microbial cellulases are industrially used enzymes that catalyze the cleavage
of the glycosidic bonds of cellulose. This hydrolysis yields sugars that can be
used in processes such as bioethanol production. These enzymes are mainly
produced by fungi belonging to the genus Trichoderma via submerged or solid
state fermentation with cellulosic materials as substrates. Recent publications
have increasingly demonstrated that alternatives to T. reesei enzymes in the
production of second-generation biofuels exist. Here, cellulolytic activities of
crude extracts obtained from a native isolate of T. asperellum from coffe pulp
and a strain of T. reesei were evaluated. Solid state fermentations were performed
using paper and sawdust as substrates. The activities were measured after
12 days of incubation. The extracts obtained from T. reesei showed higher
cellulase and endoglucanase activities (6.5 and 5.8 U/g) than those obtained
using T. asperellum (5.6 and 4.1 U/g) with paper as substrate. There were
no significant differences between isolates when grown on sawdust. It was
possible to verify that native T. asperellum was able to produce cellulases on
lignocellulosic material such as moistened paper and sawdust without having
undergone a chemical pretreatment. |
| format | Article |
| id | doaj-art-31d47cf6c8f64c82b7b196a33de38b2d |
| institution | OA Journals |
| issn | 0122-7483 2027-1352 |
| language | English |
| publishDate | 2018-11-01 |
| publisher | Pontificia Universidad Javeriana |
| record_format | Article |
| series | Universitas Scientiarum |
| spelling | doaj-art-31d47cf6c8f64c82b7b196a33de38b2d2025-08-20T02:27:57ZengPontificia Universidad JaverianaUniversitas Scientiarum0122-74832027-13522018-11-0123341943610.11144/Javeriana.SC23-3.cpopCellulases production on paper and sawdust using native Trichoderma asperellumY-Maritza Zapata, Angelica Galviz-Quezada, Víctor-Manuel Osorio-Echeverri0. Semillero de investigación sifacs, Facultad de Ciencias de la Salud, Institución Universitaria Colegio Mayor de Antioquia, Carrera 78 # 65-46, Medellín, Colombia, Grupo Biociencias, Facultad de Ciencias de la Salud, Institución Universitaria Colegio Mayor de Antioquia, Carrera 78 # 65-46, Medellín, Colombia.Microbial cellulases are industrially used enzymes that catalyze the cleavage of the glycosidic bonds of cellulose. This hydrolysis yields sugars that can be used in processes such as bioethanol production. These enzymes are mainly produced by fungi belonging to the genus Trichoderma via submerged or solid state fermentation with cellulosic materials as substrates. Recent publications have increasingly demonstrated that alternatives to T. reesei enzymes in the production of second-generation biofuels exist. Here, cellulolytic activities of crude extracts obtained from a native isolate of T. asperellum from coffe pulp and a strain of T. reesei were evaluated. Solid state fermentations were performed using paper and sawdust as substrates. The activities were measured after 12 days of incubation. The extracts obtained from T. reesei showed higher cellulase and endoglucanase activities (6.5 and 5.8 U/g) than those obtained using T. asperellum (5.6 and 4.1 U/g) with paper as substrate. There were no significant differences between isolates when grown on sawdust. It was possible to verify that native T. asperellum was able to produce cellulases on lignocellulosic material such as moistened paper and sawdust without having undergone a chemical pretreatment.https://revistas.javeriana.edu.co/index.php/scientarium/article/view/19652cellulases; cellulolytic extracts; solid state fermentation; trichoderma. |
| spellingShingle | Y-Maritza Zapata, Angelica Galviz-Quezada, Víctor-Manuel Osorio-Echeverri Cellulases production on paper and sawdust using native Trichoderma asperellum Universitas Scientiarum cellulases; cellulolytic extracts; solid state fermentation; trichoderma. |
| title | Cellulases production on paper and sawdust using native Trichoderma asperellum |
| title_full | Cellulases production on paper and sawdust using native Trichoderma asperellum |
| title_fullStr | Cellulases production on paper and sawdust using native Trichoderma asperellum |
| title_full_unstemmed | Cellulases production on paper and sawdust using native Trichoderma asperellum |
| title_short | Cellulases production on paper and sawdust using native Trichoderma asperellum |
| title_sort | cellulases production on paper and sawdust using native trichoderma asperellum |
| topic | cellulases; cellulolytic extracts; solid state fermentation; trichoderma. |
| url | https://revistas.javeriana.edu.co/index.php/scientarium/article/view/19652 |
| work_keys_str_mv | AT ymaritzazapataangelicagalvizquezadavictormanuelosorioecheverri cellulasesproductiononpaperandsawdustusingnativetrichodermaasperellum |