Efficacy of umbilical cord-derived mesenchymal stem cells and exosomes in conjunction with standard IBD drug on immune responses in an IBD mouse model
Abstract Background Inflammatory bowel disease (IBD) is a persistent inflammation of the digestive system, and Mesenchymal Stem Cells (MSCs) and their exosomes have demonstrated potential as treatments for this condition. The objective of this research was to examine the possible effectiveness of in...
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BMC
2025-01-01
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| Series: | Stem Cell Research & Therapy |
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| Online Access: | https://doi.org/10.1186/s13287-024-04062-y |
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| author | Fatemeh Kheradmand Seyedeh Fatemeh Yasaman Rahimzadeh Seyed-Alireza Esmaeili Sajad Sahab Negah Najmeh Kaffash Farkhad Seyedeh Elnaz Nazari Mehrdad Hajinejad Mohammad Ali Khodadoust Afsane Fadaee Jalil Tavakol Afshari Majid Khazaei |
| author_facet | Fatemeh Kheradmand Seyedeh Fatemeh Yasaman Rahimzadeh Seyed-Alireza Esmaeili Sajad Sahab Negah Najmeh Kaffash Farkhad Seyedeh Elnaz Nazari Mehrdad Hajinejad Mohammad Ali Khodadoust Afsane Fadaee Jalil Tavakol Afshari Majid Khazaei |
| author_sort | Fatemeh Kheradmand |
| collection | DOAJ |
| description | Abstract Background Inflammatory bowel disease (IBD) is a persistent inflammation of the digestive system, and Mesenchymal Stem Cells (MSCs) and their exosomes have demonstrated potential as treatments for this condition. The objective of this research was to examine the possible effectiveness of intraperitoneal injection of umbilical cord-MSCs (UC-MSCs) and their exosomes through a two-time injection regimen in a mouse model. Method In this study, an animal model of a specific type of IBD in C57BL/6 mice, induced by dextran sulfate sodium (DSS), was utilized. The mice were treated with MSCs, exosomes, Mesalazine, and a combination of them. Upon sacrificing the mice, colon and spleen tissues were isolated to assess the changes in the mice’s weight, colon length, spleen weight, and colitis’ pathological symptoms. IL-10 and IL-17 levels were measured, and Treg and Th17 cell percentages were determined as well. Furthermore, colon tissue was stained to investigate histopathological changes. Results In the groups that received MSCs, there was a significant reduction in the disease activity index and their combinations with exosomes and Mesalazine compared to the colitis group. Colon length increased in all groups except the exosome group. Histological measures were notably reduced in the MSC groups and their combinations. Significant increases in the IL-10 level of colon tissue and the proportion of Treg present in the spleen were observed in the groups receiving MSC and combination treatment. Furthermore, these groups showed a notable reduction in the percentage of spleen Th17 cells. However, IL17A decreased non-significantly in all groups. Conclusion The results showed that intraperitoneal injection of UC-MSCs and their combination with exosome and Mesalazine in a murine colitis model improved the disease’s symptoms. Therefore, MSCs and their combination with exosomes can be a promising therapeutic approach along with other common drugs for IBD, but exosomes alone could not significantly reduce the symptoms of colitis. |
| format | Article |
| id | doaj-art-31d0b1b363bb40389e532cca1afa4073 |
| institution | Kabale University |
| issn | 1757-6512 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
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| series | Stem Cell Research & Therapy |
| spelling | doaj-art-31d0b1b363bb40389e532cca1afa40732025-01-12T12:10:18ZengBMCStem Cell Research & Therapy1757-65122025-01-0116111510.1186/s13287-024-04062-yEfficacy of umbilical cord-derived mesenchymal stem cells and exosomes in conjunction with standard IBD drug on immune responses in an IBD mouse modelFatemeh Kheradmand0Seyedeh Fatemeh Yasaman Rahimzadeh1Seyed-Alireza Esmaeili2Sajad Sahab Negah3Najmeh Kaffash Farkhad4Seyedeh Elnaz Nazari5Mehrdad Hajinejad6Mohammad Ali Khodadoust7Afsane Fadaee8Jalil Tavakol Afshari9Majid Khazaei10Immunology Research Center, Mashhad University of Medical SciencesImmunology Research Center, Mashhad University of Medical SciencesImmunology Research Center, Mashhad University of Medical SciencesNeuroscience Research Center, Mashhad University of Medical SciencesImmunology Research Center, Mashhad University of Medical SciencesDepartment of Medical Physiology, Faculty of Medicine, Mashhad University of Medical SciencesNeuroscience Research Center, Mashhad University of Medical SciencesImmunology Research Center, Mashhad University of Medical SciencesImmunology Research Center, Inflammation and Inflammatory Diseases Division, Mashhad University of Medical SciencesImmunology Research Center, Mashhad University of Medical SciencesDepartment of Medical Physiology, Faculty of Medicine, Mashhad University of Medical SciencesAbstract Background Inflammatory bowel disease (IBD) is a persistent inflammation of the digestive system, and Mesenchymal Stem Cells (MSCs) and their exosomes have demonstrated potential as treatments for this condition. The objective of this research was to examine the possible effectiveness of intraperitoneal injection of umbilical cord-MSCs (UC-MSCs) and their exosomes through a two-time injection regimen in a mouse model. Method In this study, an animal model of a specific type of IBD in C57BL/6 mice, induced by dextran sulfate sodium (DSS), was utilized. The mice were treated with MSCs, exosomes, Mesalazine, and a combination of them. Upon sacrificing the mice, colon and spleen tissues were isolated to assess the changes in the mice’s weight, colon length, spleen weight, and colitis’ pathological symptoms. IL-10 and IL-17 levels were measured, and Treg and Th17 cell percentages were determined as well. Furthermore, colon tissue was stained to investigate histopathological changes. Results In the groups that received MSCs, there was a significant reduction in the disease activity index and their combinations with exosomes and Mesalazine compared to the colitis group. Colon length increased in all groups except the exosome group. Histological measures were notably reduced in the MSC groups and their combinations. Significant increases in the IL-10 level of colon tissue and the proportion of Treg present in the spleen were observed in the groups receiving MSC and combination treatment. Furthermore, these groups showed a notable reduction in the percentage of spleen Th17 cells. However, IL17A decreased non-significantly in all groups. Conclusion The results showed that intraperitoneal injection of UC-MSCs and their combination with exosome and Mesalazine in a murine colitis model improved the disease’s symptoms. Therefore, MSCs and their combination with exosomes can be a promising therapeutic approach along with other common drugs for IBD, but exosomes alone could not significantly reduce the symptoms of colitis.https://doi.org/10.1186/s13287-024-04062-yMesenchymal stem cellsExosomeInflammatory bowel disease |
| spellingShingle | Fatemeh Kheradmand Seyedeh Fatemeh Yasaman Rahimzadeh Seyed-Alireza Esmaeili Sajad Sahab Negah Najmeh Kaffash Farkhad Seyedeh Elnaz Nazari Mehrdad Hajinejad Mohammad Ali Khodadoust Afsane Fadaee Jalil Tavakol Afshari Majid Khazaei Efficacy of umbilical cord-derived mesenchymal stem cells and exosomes in conjunction with standard IBD drug on immune responses in an IBD mouse model Stem Cell Research & Therapy Mesenchymal stem cells Exosome Inflammatory bowel disease |
| title | Efficacy of umbilical cord-derived mesenchymal stem cells and exosomes in conjunction with standard IBD drug on immune responses in an IBD mouse model |
| title_full | Efficacy of umbilical cord-derived mesenchymal stem cells and exosomes in conjunction with standard IBD drug on immune responses in an IBD mouse model |
| title_fullStr | Efficacy of umbilical cord-derived mesenchymal stem cells and exosomes in conjunction with standard IBD drug on immune responses in an IBD mouse model |
| title_full_unstemmed | Efficacy of umbilical cord-derived mesenchymal stem cells and exosomes in conjunction with standard IBD drug on immune responses in an IBD mouse model |
| title_short | Efficacy of umbilical cord-derived mesenchymal stem cells and exosomes in conjunction with standard IBD drug on immune responses in an IBD mouse model |
| title_sort | efficacy of umbilical cord derived mesenchymal stem cells and exosomes in conjunction with standard ibd drug on immune responses in an ibd mouse model |
| topic | Mesenchymal stem cells Exosome Inflammatory bowel disease |
| url | https://doi.org/10.1186/s13287-024-04062-y |
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