The genome of the sapphire damselfish Chrysiptera cyanea: a new resource to support further investigation of the evolution of Pomacentrids
The number of high-quality genomes is rapidly increasing across taxa. However, it remains limited for coral reef fish of the Pomacentrid family, with most research focused on anemonefish. Here, we present the first assembly for a Pomacentrid of the genus Chrysiptera. Using PacBio long-read sequenci...
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GigaScience Press
2024-12-01
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| Series: | GigaByte |
| Online Access: | https://gigabytejournal.com/articles/144 |
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| author | Emma Gairin Saori Miura Hiroki Takamiyagi Marcela Herrera Vincent Laudet |
| author_facet | Emma Gairin Saori Miura Hiroki Takamiyagi Marcela Herrera Vincent Laudet |
| author_sort | Emma Gairin |
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The number of high-quality genomes is rapidly increasing across taxa. However, it remains limited for coral reef fish of the Pomacentrid family, with most research focused on anemonefish. Here, we present the first assembly for a Pomacentrid of the genus Chrysiptera. Using PacBio long-read sequencing with 94.5× coverage, the genome of the Sapphire Devil, Chrysiptera cyanea, was assembled and annotated. The final assembly comprises 896 Mb pairs across 91 contigs, with a BUSCO completeness of 97.6%, and 28,173 genes. Comparative analyses with chromosome-scale assemblies of related species identified contig-chromosome correspondences. This genome will be useful as a comparison to study specific adaptations linked to the symbiotic life of closely related anemonefish. Furthermore, C. cyanea is found in most tropical coastal areas of the Indo-West Pacific and could become a model for environmental monitoring. This work will expand coral reef research efforts, highlighting the power of long-read assemblies to retrieve high quality genomes.
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| format | Article |
| id | doaj-art-31cdd6995c5d46bcb3cd595e488a19c5 |
| institution | DOAJ |
| issn | 2709-4715 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | GigaScience Press |
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| series | GigaByte |
| spelling | doaj-art-31cdd6995c5d46bcb3cd595e488a19c52025-08-20T03:12:53ZengGigaScience PressGigaByte2709-47152024-12-0110.46471/gigabyte.144The genome of the sapphire damselfish Chrysiptera cyanea: a new resource to support further investigation of the evolution of PomacentridsEmma Gairin 0https://orcid.org/0000-0001-6898-8084Saori Miura 1Hiroki Takamiyagi 2Marcela Herrera 3https://orcid.org/0000-0001-6021-3989Vincent Laudet 4https://orcid.org/0000-0003-4022-4175Marine Eco-Evo-Devo Unit, https://ror.org/02qg15b79Okinawa Institute of Science and Technology, 904-0495, Onna-son, Okinawa, JapanMarine Eco-Evo-Devo Unit, https://ror.org/02qg15b79Okinawa Institute of Science and Technology, 904-0495, Onna-son, Okinawa, JapanMarine Eco-Evo-Devo Unit, https://ror.org/02qg15b79Okinawa Institute of Science and Technology, 904-0495, Onna-son, Okinawa, JapanMarine Eco-Evo-Devo Unit, https://ror.org/02qg15b79Okinawa Institute of Science and Technology, 904-0495, Onna-son, Okinawa, JapanMarine Eco-Evo-Devo Unit, https://ror.org/02qg15b79Okinawa Institute of Science and Technology, 904-0495, Onna-son, Okinawa, Japan, Marine Research Station, https://ror.org/048evbw70Institute of Cellular and Organismic Biology, Academia Sinica, 23-10, Dah-Uen Rd, Jiau Shi, I-Lan 262, Taiwan, CNRS IRL 2028 “Eco-Evo-Devo of Coral Reef Fish Life Cycle” (EARLY), France The number of high-quality genomes is rapidly increasing across taxa. However, it remains limited for coral reef fish of the Pomacentrid family, with most research focused on anemonefish. Here, we present the first assembly for a Pomacentrid of the genus Chrysiptera. Using PacBio long-read sequencing with 94.5× coverage, the genome of the Sapphire Devil, Chrysiptera cyanea, was assembled and annotated. The final assembly comprises 896 Mb pairs across 91 contigs, with a BUSCO completeness of 97.6%, and 28,173 genes. Comparative analyses with chromosome-scale assemblies of related species identified contig-chromosome correspondences. This genome will be useful as a comparison to study specific adaptations linked to the symbiotic life of closely related anemonefish. Furthermore, C. cyanea is found in most tropical coastal areas of the Indo-West Pacific and could become a model for environmental monitoring. This work will expand coral reef research efforts, highlighting the power of long-read assemblies to retrieve high quality genomes. https://gigabytejournal.com/articles/144 |
| spellingShingle | Emma Gairin Saori Miura Hiroki Takamiyagi Marcela Herrera Vincent Laudet The genome of the sapphire damselfish Chrysiptera cyanea: a new resource to support further investigation of the evolution of Pomacentrids GigaByte |
| title | The genome of the sapphire damselfish Chrysiptera cyanea: a new resource to support further investigation of the evolution of Pomacentrids |
| title_full | The genome of the sapphire damselfish Chrysiptera cyanea: a new resource to support further investigation of the evolution of Pomacentrids |
| title_fullStr | The genome of the sapphire damselfish Chrysiptera cyanea: a new resource to support further investigation of the evolution of Pomacentrids |
| title_full_unstemmed | The genome of the sapphire damselfish Chrysiptera cyanea: a new resource to support further investigation of the evolution of Pomacentrids |
| title_short | The genome of the sapphire damselfish Chrysiptera cyanea: a new resource to support further investigation of the evolution of Pomacentrids |
| title_sort | genome of the sapphire damselfish chrysiptera cyanea a new resource to support further investigation of the evolution of pomacentrids |
| url | https://gigabytejournal.com/articles/144 |
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