Single-cell transcriptomics reveals an abnormal immune microenvironment in plasma cell mastitis
Introduction Plasma cell mastitis (PCM) is a non-bacterial inflammatory breast disorder affecting young and middle-aged nonpregnant and nonlactating women. However, a comprehensive and systematic analysis of PCM has not been conducted.Methods and Results Comprehensive cellular indexing of transcript...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2025-12-01
|
| Series: | Annals of Medicine |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/07853890.2024.2446694 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850052226373910528 |
|---|---|
| author | Qingtao Ni Gaohua Han Chi Pan |
| author_facet | Qingtao Ni Gaohua Han Chi Pan |
| author_sort | Qingtao Ni |
| collection | DOAJ |
| description | Introduction Plasma cell mastitis (PCM) is a non-bacterial inflammatory breast disorder affecting young and middle-aged nonpregnant and nonlactating women. However, a comprehensive and systematic analysis of PCM has not been conducted.Methods and Results Comprehensive cellular indexing of transcriptomes and epitopes by sequencing (CITE-seq) analysis was performed on 10,185 cells derived from PCM tissue to generate a highly detailed transcriptomic atlas. Thirteen distinct clusters were identified, and eight cell types were successfully annotated, including endothelial cells, common myeloid progenitor cells and neutrophils. Additionally, the overall alterations in immune cell composition in PCM patients were evaluated. Furthermore, differentially expressed genes between normal and PCM samples were assessed, and functional enrichment analysis was conducted. Finally, cellular communication and interaction were analysed to determine cell-to-cell communications.Conclusion This study significantly contributes to the field of PCM, presenting a systematic and valuable perspective that serves as a fundamental resource for further exploration of the cellular and molecular mechanisms, pathogenesis of associated diseases and potential interventions. |
| format | Article |
| id | doaj-art-31c1e3ffbafe428c8db3b84a5d368a7b |
| institution | DOAJ |
| issn | 0785-3890 1365-2060 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Annals of Medicine |
| spelling | doaj-art-31c1e3ffbafe428c8db3b84a5d368a7b2025-08-20T02:52:53ZengTaylor & Francis GroupAnnals of Medicine0785-38901365-20602025-12-0157110.1080/07853890.2024.2446694Single-cell transcriptomics reveals an abnormal immune microenvironment in plasma cell mastitisQingtao Ni0Gaohua Han1Chi Pan2Department of Oncology, The Affiliated Taizhou People’s Hospital of Nanjing Medical University, Taizhou School of Clinical Medicine, Nanjing Medical University, Taizhou, ChinaDepartment of Oncology, The Affiliated Taizhou People’s Hospital of Nanjing Medical University, Taizhou School of Clinical Medicine, Nanjing Medical University, Taizhou, ChinaDepartment of General Surgery, The Affiliated Taizhou People’s Hospital of Nanjing Medical University, Taizhou School of Clinical Medicine, Nanjing Medical University, Taizhou, ChinaIntroduction Plasma cell mastitis (PCM) is a non-bacterial inflammatory breast disorder affecting young and middle-aged nonpregnant and nonlactating women. However, a comprehensive and systematic analysis of PCM has not been conducted.Methods and Results Comprehensive cellular indexing of transcriptomes and epitopes by sequencing (CITE-seq) analysis was performed on 10,185 cells derived from PCM tissue to generate a highly detailed transcriptomic atlas. Thirteen distinct clusters were identified, and eight cell types were successfully annotated, including endothelial cells, common myeloid progenitor cells and neutrophils. Additionally, the overall alterations in immune cell composition in PCM patients were evaluated. Furthermore, differentially expressed genes between normal and PCM samples were assessed, and functional enrichment analysis was conducted. Finally, cellular communication and interaction were analysed to determine cell-to-cell communications.Conclusion This study significantly contributes to the field of PCM, presenting a systematic and valuable perspective that serves as a fundamental resource for further exploration of the cellular and molecular mechanisms, pathogenesis of associated diseases and potential interventions.https://www.tandfonline.com/doi/10.1080/07853890.2024.2446694Single-cell transcriptomicsimmune microenvironmentsignalling pathwayplasma cell mastitis |
| spellingShingle | Qingtao Ni Gaohua Han Chi Pan Single-cell transcriptomics reveals an abnormal immune microenvironment in plasma cell mastitis Annals of Medicine Single-cell transcriptomics immune microenvironment signalling pathway plasma cell mastitis |
| title | Single-cell transcriptomics reveals an abnormal immune microenvironment in plasma cell mastitis |
| title_full | Single-cell transcriptomics reveals an abnormal immune microenvironment in plasma cell mastitis |
| title_fullStr | Single-cell transcriptomics reveals an abnormal immune microenvironment in plasma cell mastitis |
| title_full_unstemmed | Single-cell transcriptomics reveals an abnormal immune microenvironment in plasma cell mastitis |
| title_short | Single-cell transcriptomics reveals an abnormal immune microenvironment in plasma cell mastitis |
| title_sort | single cell transcriptomics reveals an abnormal immune microenvironment in plasma cell mastitis |
| topic | Single-cell transcriptomics immune microenvironment signalling pathway plasma cell mastitis |
| url | https://www.tandfonline.com/doi/10.1080/07853890.2024.2446694 |
| work_keys_str_mv | AT qingtaoni singlecelltranscriptomicsrevealsanabnormalimmunemicroenvironmentinplasmacellmastitis AT gaohuahan singlecelltranscriptomicsrevealsanabnormalimmunemicroenvironmentinplasmacellmastitis AT chipan singlecelltranscriptomicsrevealsanabnormalimmunemicroenvironmentinplasmacellmastitis |