Anti-tumor effect and immune-related mechanism study of compound aluminum sulfate injection in transplanted tumor-bearing mice
This study investigates the antitumor and immunomodulatory effects of compound aluminum sulfate (CAS) solution in murine melanoma models. Using syngeneic B16-F10 and B16-OVA tumor models, we demonstrate that intratumoral CAS injection significantly inhibits primary tumor growth and lung metastasis....
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-05-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1583275/full |
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| author | Zhenwei Shi Zhenwei Shi Zhifa Xia Zhifa Xia Songtao Huang Songtao Huang Zeteng Chen Zeteng Chen Fan Yin Haili Xin Fenghua Xu |
| author_facet | Zhenwei Shi Zhenwei Shi Zhifa Xia Zhifa Xia Songtao Huang Songtao Huang Zeteng Chen Zeteng Chen Fan Yin Haili Xin Fenghua Xu |
| author_sort | Zhenwei Shi |
| collection | DOAJ |
| description | This study investigates the antitumor and immunomodulatory effects of compound aluminum sulfate (CAS) solution in murine melanoma models. Using syngeneic B16-F10 and B16-OVA tumor models, we demonstrate that intratumoral CAS injection significantly inhibits primary tumor growth and lung metastasis. Flow cytometry analysis reveals that CAS treatment increases splenic populations of CD3+CD8+ cytotoxic T cells, CD3+CD44+ memory T cells, and NK cells, while enhancing CD8+ T cell infiltration in tumor tissue. ELISA results show elevated levels of pro-inflammatory cytokines (IFN-γ, TNF-α, and IL-2) in splenic culture supernatants and serum following CAS administration. Immunofluorescence staining confirms increased expression of CD8 and IFN-γ proteins in tumor tissues of CAS-treated mice. Results indicate that CAS exerts its antitumor effects through direct cytotoxicity and by modulating both systemic and local immune responses. The dual action of CAS, which combines tumor necrosis with immunostimulation, positions it as a promising therapeutic agent for cancer treatment. This study offers valuable insights into the mechanisms underlying CAS’s action and underscores its potential clinical applications in oncology. |
| format | Article |
| id | doaj-art-31b3fe9931ea4cd6ad7bc5ba5d4944e5 |
| institution | OA Journals |
| issn | 1664-3224 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj-art-31b3fe9931ea4cd6ad7bc5ba5d4944e52025-08-20T01:48:40ZengFrontiers Media S.A.Frontiers in Immunology1664-32242025-05-011610.3389/fimmu.2025.15832751583275Anti-tumor effect and immune-related mechanism study of compound aluminum sulfate injection in transplanted tumor-bearing miceZhenwei Shi0Zhenwei Shi1Zhifa Xia2Zhifa Xia3Songtao Huang4Songtao Huang5Zeteng Chen6Zeteng Chen7Fan Yin8Haili Xin9Fenghua Xu10Medical School of Chinese People’s Liberation Army General Hospital, Beijing, ChinaPharmaceutical Sciences Research Division, Department of Pharmacy, Medical Supplies Center, Chinese People's Liberation Army General Hospital, Beijing, ChinaMedical School of Chinese People’s Liberation Army General Hospital, Beijing, ChinaPharmaceutical Sciences Research Division, Department of Pharmacy, Medical Supplies Center, Chinese People's Liberation Army General Hospital, Beijing, ChinaMedical School of Chinese People’s Liberation Army General Hospital, Beijing, ChinaPharmaceutical Sciences Research Division, Department of Pharmacy, Medical Supplies Center, Chinese People's Liberation Army General Hospital, Beijing, ChinaMedical School of Chinese People’s Liberation Army General Hospital, Beijing, ChinaPharmaceutical Sciences Research Division, Department of Pharmacy, Medical Supplies Center, Chinese People's Liberation Army General Hospital, Beijing, ChinaThe Second Medical Center, Chinese People's Liberation Army General Hospital, Beijing, ChinaDepartment of Pharmacy, Medical Supplies Center, Chinese People's Liberation Army General Hospital, Beijing, ChinaPharmaceutical Sciences Research Division, Department of Pharmacy, Medical Supplies Center, Chinese People's Liberation Army General Hospital, Beijing, ChinaThis study investigates the antitumor and immunomodulatory effects of compound aluminum sulfate (CAS) solution in murine melanoma models. Using syngeneic B16-F10 and B16-OVA tumor models, we demonstrate that intratumoral CAS injection significantly inhibits primary tumor growth and lung metastasis. Flow cytometry analysis reveals that CAS treatment increases splenic populations of CD3+CD8+ cytotoxic T cells, CD3+CD44+ memory T cells, and NK cells, while enhancing CD8+ T cell infiltration in tumor tissue. ELISA results show elevated levels of pro-inflammatory cytokines (IFN-γ, TNF-α, and IL-2) in splenic culture supernatants and serum following CAS administration. Immunofluorescence staining confirms increased expression of CD8 and IFN-γ proteins in tumor tissues of CAS-treated mice. Results indicate that CAS exerts its antitumor effects through direct cytotoxicity and by modulating both systemic and local immune responses. The dual action of CAS, which combines tumor necrosis with immunostimulation, positions it as a promising therapeutic agent for cancer treatment. This study offers valuable insights into the mechanisms underlying CAS’s action and underscores its potential clinical applications in oncology.https://www.frontiersin.org/articles/10.3389/fimmu.2025.1583275/fullcompound aluminum sulfate injectionanti-tumor effectmelanomaimmunomodulatormetastasis |
| spellingShingle | Zhenwei Shi Zhenwei Shi Zhifa Xia Zhifa Xia Songtao Huang Songtao Huang Zeteng Chen Zeteng Chen Fan Yin Haili Xin Fenghua Xu Anti-tumor effect and immune-related mechanism study of compound aluminum sulfate injection in transplanted tumor-bearing mice Frontiers in Immunology compound aluminum sulfate injection anti-tumor effect melanoma immunomodulator metastasis |
| title | Anti-tumor effect and immune-related mechanism study of compound aluminum sulfate injection in transplanted tumor-bearing mice |
| title_full | Anti-tumor effect and immune-related mechanism study of compound aluminum sulfate injection in transplanted tumor-bearing mice |
| title_fullStr | Anti-tumor effect and immune-related mechanism study of compound aluminum sulfate injection in transplanted tumor-bearing mice |
| title_full_unstemmed | Anti-tumor effect and immune-related mechanism study of compound aluminum sulfate injection in transplanted tumor-bearing mice |
| title_short | Anti-tumor effect and immune-related mechanism study of compound aluminum sulfate injection in transplanted tumor-bearing mice |
| title_sort | anti tumor effect and immune related mechanism study of compound aluminum sulfate injection in transplanted tumor bearing mice |
| topic | compound aluminum sulfate injection anti-tumor effect melanoma immunomodulator metastasis |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1583275/full |
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