Genetic Diversity and Evaluation of Agro-Morphological Traits in Lettuce Core Collection

Lettuce (<i>Lactuca sativa</i>) is a globally significant leafy vegetable, valued for both its economic and nutritional contributions. The efficient conservation and use of the lettuce germplasm are crucial for breeding and genetic improvement. This study examined the genetic diversity a...

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Main Authors: Suyun Moon, Onsook Hur, Seong-Hoon Kim, Yoonjung Lee, Hyeonseok Oh, Jungyoon Yi, Ho-Cheol Ko, Hee-Jong Woo, Nayoung Ro, Young-Wang Na
Format: Article
Language:English
Published: MDPI AG 2024-12-01
Series:Plants
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Online Access:https://www.mdpi.com/2223-7747/13/24/3552
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author Suyun Moon
Onsook Hur
Seong-Hoon Kim
Yoonjung Lee
Hyeonseok Oh
Jungyoon Yi
Ho-Cheol Ko
Hee-Jong Woo
Nayoung Ro
Young-Wang Na
author_facet Suyun Moon
Onsook Hur
Seong-Hoon Kim
Yoonjung Lee
Hyeonseok Oh
Jungyoon Yi
Ho-Cheol Ko
Hee-Jong Woo
Nayoung Ro
Young-Wang Na
author_sort Suyun Moon
collection DOAJ
description Lettuce (<i>Lactuca sativa</i>) is a globally significant leafy vegetable, valued for both its economic and nutritional contributions. The efficient conservation and use of the lettuce germplasm are crucial for breeding and genetic improvement. This study examined the genetic diversity and population structure of a core collection of the lettuce germplasm using genotyping by sequencing (GBS). A total of 7136 high-quality single-nucleotide polymorphisms (SNPs) were identified across nine chromosomes. Population analysis through Bayesian clustering and discriminant analysis of principal components (DAPC) revealed three distinct genetic clusters. Cluster 2 exhibited the greatest genetic diversity (<i>He</i> = 0.29, <i>I</i> = 0.44), while Cluster 3 had high levels of inbreeding (<i>F</i> = 0.79). Agro-morphological trait evaluation further identified significant differences in leaf length, plant weight, and head height across clusters. These findings provide valuable insights into the genetic and phenotypic diversity of lettuce, facilitating the development of more robust breeding programs. Additionally, the core collection established in this study offers a representative subset of the lettuce germplasm for future genomic research and conservation efforts.
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spelling doaj-art-31b189b6db8d46a2b6e932b644a0586c2025-08-20T02:01:28ZengMDPI AGPlants2223-77472024-12-011324355210.3390/plants13243552Genetic Diversity and Evaluation of Agro-Morphological Traits in Lettuce Core CollectionSuyun Moon0Onsook Hur1Seong-Hoon Kim2Yoonjung Lee3Hyeonseok Oh4Jungyoon Yi5Ho-Cheol Ko6Hee-Jong Woo7Nayoung Ro8Young-Wang Na9National Agrobiodiversity Center, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 54874, Republic of KoreaDepartment of Crop Breeding, National Institute of Crop Science, Rural Development Administration, Wanju 55365, Republic of KoreaNational Agrobiodiversity Center, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 54874, Republic of KoreaNational Agrobiodiversity Center, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 54874, Republic of KoreaNational Agrobiodiversity Center, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 54874, Republic of KoreaNational Agrobiodiversity Center, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 54874, Republic of KoreaNational Agrobiodiversity Center, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 54874, Republic of KoreaNational Agrobiodiversity Center, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 54874, Republic of KoreaNational Agrobiodiversity Center, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 54874, Republic of KoreaNational Agrobiodiversity Center, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 54874, Republic of KoreaLettuce (<i>Lactuca sativa</i>) is a globally significant leafy vegetable, valued for both its economic and nutritional contributions. The efficient conservation and use of the lettuce germplasm are crucial for breeding and genetic improvement. This study examined the genetic diversity and population structure of a core collection of the lettuce germplasm using genotyping by sequencing (GBS). A total of 7136 high-quality single-nucleotide polymorphisms (SNPs) were identified across nine chromosomes. Population analysis through Bayesian clustering and discriminant analysis of principal components (DAPC) revealed three distinct genetic clusters. Cluster 2 exhibited the greatest genetic diversity (<i>He</i> = 0.29, <i>I</i> = 0.44), while Cluster 3 had high levels of inbreeding (<i>F</i> = 0.79). Agro-morphological trait evaluation further identified significant differences in leaf length, plant weight, and head height across clusters. These findings provide valuable insights into the genetic and phenotypic diversity of lettuce, facilitating the development of more robust breeding programs. Additionally, the core collection established in this study offers a representative subset of the lettuce germplasm for future genomic research and conservation efforts.https://www.mdpi.com/2223-7747/13/24/3552<i>Lactuca sativa</i>germplasmgenetic diversitypopulation structureagro-morphological characterizationgenotyping by sequencing
spellingShingle Suyun Moon
Onsook Hur
Seong-Hoon Kim
Yoonjung Lee
Hyeonseok Oh
Jungyoon Yi
Ho-Cheol Ko
Hee-Jong Woo
Nayoung Ro
Young-Wang Na
Genetic Diversity and Evaluation of Agro-Morphological Traits in Lettuce Core Collection
Plants
<i>Lactuca sativa</i>
germplasm
genetic diversity
population structure
agro-morphological characterization
genotyping by sequencing
title Genetic Diversity and Evaluation of Agro-Morphological Traits in Lettuce Core Collection
title_full Genetic Diversity and Evaluation of Agro-Morphological Traits in Lettuce Core Collection
title_fullStr Genetic Diversity and Evaluation of Agro-Morphological Traits in Lettuce Core Collection
title_full_unstemmed Genetic Diversity and Evaluation of Agro-Morphological Traits in Lettuce Core Collection
title_short Genetic Diversity and Evaluation of Agro-Morphological Traits in Lettuce Core Collection
title_sort genetic diversity and evaluation of agro morphological traits in lettuce core collection
topic <i>Lactuca sativa</i>
germplasm
genetic diversity
population structure
agro-morphological characterization
genotyping by sequencing
url https://www.mdpi.com/2223-7747/13/24/3552
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