Quenching-Chemiluminescence Determination of Trace Amounts of l-Tyrosine Contained in Dietary Supplement by Chemiluminescence Reaction of an Iron-Phthalocyanine Complex
The chemiluminescence (CL) signal immediately appeared when a hydrogen peroxide solution was injected into an iron-phthalocyanine tetrasulfonic acid (Fe-PTS) aqueous solution. Moreover, the CL intensity of Fe-PTS decreased by adding L-tyrosine. Based on these results, the determination of trace amo...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2012/520248 |
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| author | Takao Ohtomo Shukuro Igarashi Yoshitaka Takagai Osamu Ohno |
| author_facet | Takao Ohtomo Shukuro Igarashi Yoshitaka Takagai Osamu Ohno |
| author_sort | Takao Ohtomo |
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| description | The chemiluminescence (CL) signal immediately appeared when a hydrogen peroxide solution was injected into an iron-phthalocyanine tetrasulfonic acid (Fe-PTS) aqueous solution. Moreover, the CL intensity of Fe-PTS decreased by adding L-tyrosine. Based on these results, the determination of trace amounts of L-tyrosine was developed using the quenching-chemiluminescence. The calibration curve of L-tyrosine was obtained in the concentration range of 2.0×10−7 M to 2.0×10−5 M. Moreover, the relative standard deviation (RSD) was 1.63 % (𝑛=5) for 2.0×10−6 M L-tyrosine, and its detection limits (3σ) were 1.81×10−7 M. The spike and recovery experiments for L-tyrosine were performed using a soft drink. Furthermore, the determination of L-tyrosine was applied to supplements containing various kinds of amino acids. Each satisfactory relative recovery was obtained at 98 to 102%. |
| format | Article |
| id | doaj-art-3193263bfab3461bb5d3636a97aebec5 |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-3193263bfab3461bb5d3636a97aebec52025-02-03T06:13:19ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732012-01-01201210.1155/2012/520248520248Quenching-Chemiluminescence Determination of Trace Amounts of l-Tyrosine Contained in Dietary Supplement by Chemiluminescence Reaction of an Iron-Phthalocyanine ComplexTakao Ohtomo0Shukuro Igarashi1Yoshitaka Takagai2Osamu Ohno3Department of Biomolecular Functional Engineering, Faculty of Engineering, Ibaraki University, 4-12-1 Nakanarusawa, Hitachi, Ibaraki 316-8511, JapanDepartment of Biomolecular Functional Engineering, Faculty of Engineering, Ibaraki University, 4-12-1 Nakanarusawa, Hitachi, Ibaraki 316-8511, JapanFaculty of Symbiotic Systems Science, Cluster of Science and Technology, Fukushima University, Kanayagawa 1, Fukushima 960-1296, JapanDepartment of Biomolecular Functional Engineering, Faculty of Engineering, Ibaraki University, 4-12-1 Nakanarusawa, Hitachi, Ibaraki 316-8511, JapanThe chemiluminescence (CL) signal immediately appeared when a hydrogen peroxide solution was injected into an iron-phthalocyanine tetrasulfonic acid (Fe-PTS) aqueous solution. Moreover, the CL intensity of Fe-PTS decreased by adding L-tyrosine. Based on these results, the determination of trace amounts of L-tyrosine was developed using the quenching-chemiluminescence. The calibration curve of L-tyrosine was obtained in the concentration range of 2.0×10−7 M to 2.0×10−5 M. Moreover, the relative standard deviation (RSD) was 1.63 % (𝑛=5) for 2.0×10−6 M L-tyrosine, and its detection limits (3σ) were 1.81×10−7 M. The spike and recovery experiments for L-tyrosine were performed using a soft drink. Furthermore, the determination of L-tyrosine was applied to supplements containing various kinds of amino acids. Each satisfactory relative recovery was obtained at 98 to 102%.http://dx.doi.org/10.1155/2012/520248 |
| spellingShingle | Takao Ohtomo Shukuro Igarashi Yoshitaka Takagai Osamu Ohno Quenching-Chemiluminescence Determination of Trace Amounts of l-Tyrosine Contained in Dietary Supplement by Chemiluminescence Reaction of an Iron-Phthalocyanine Complex Journal of Analytical Methods in Chemistry |
| title | Quenching-Chemiluminescence Determination of Trace Amounts of
l-Tyrosine Contained in Dietary Supplement by Chemiluminescence Reaction of an Iron-Phthalocyanine Complex |
| title_full | Quenching-Chemiluminescence Determination of Trace Amounts of
l-Tyrosine Contained in Dietary Supplement by Chemiluminescence Reaction of an Iron-Phthalocyanine Complex |
| title_fullStr | Quenching-Chemiluminescence Determination of Trace Amounts of
l-Tyrosine Contained in Dietary Supplement by Chemiluminescence Reaction of an Iron-Phthalocyanine Complex |
| title_full_unstemmed | Quenching-Chemiluminescence Determination of Trace Amounts of
l-Tyrosine Contained in Dietary Supplement by Chemiluminescence Reaction of an Iron-Phthalocyanine Complex |
| title_short | Quenching-Chemiluminescence Determination of Trace Amounts of
l-Tyrosine Contained in Dietary Supplement by Chemiluminescence Reaction of an Iron-Phthalocyanine Complex |
| title_sort | quenching chemiluminescence determination of trace amounts of l tyrosine contained in dietary supplement by chemiluminescence reaction of an iron phthalocyanine complex |
| url | http://dx.doi.org/10.1155/2012/520248 |
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