Computational design of drug candidates for influenza A virus subtype H1N1 by inhibiting the viral neuraminidase-1 enzyme

It is critical to seek potential alternative treatments for H1N1 infections by inhibiting neuraminidase-1 enzyme. One of the viable options for inhibiting the activity of neuraminidase- 1 is peptide drug design. In order to increase peptide stability, cyclization is necessary to prevent its digestio...

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Main Authors: Tambunan Usman Sumo Friend, Parikesit Arli Aditya, Dephinto Yonaniko, Sipahutar Feimmy Ruth Pratiwi
Format: Article
Language:English
Published: Sciendo 2014-06-01
Series:Acta Pharmaceutica
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Online Access:http://www.degruyter.com/view/j/acph.2014.64.issue-2/acph-2014-0015/acph-2014-0015.xml?format=INT
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author Tambunan Usman Sumo Friend
Parikesit Arli Aditya
Dephinto Yonaniko
Sipahutar Feimmy Ruth Pratiwi
author_facet Tambunan Usman Sumo Friend
Parikesit Arli Aditya
Dephinto Yonaniko
Sipahutar Feimmy Ruth Pratiwi
author_sort Tambunan Usman Sumo Friend
collection DOAJ
description It is critical to seek potential alternative treatments for H1N1 infections by inhibiting neuraminidase-1 enzyme. One of the viable options for inhibiting the activity of neuraminidase- 1 is peptide drug design. In order to increase peptide stability, cyclization is necessary to prevent its digestion by protease enzyme. Cyclization of peptide ligands by formation of disulfide bridges is preferable for designing inhibitors of neuraminidase-1 because of their high activity and specificity. Here we designed ligands by using molecular docking, drug scan and dynamics computational methods. Based on our docking results, short polypeptides of cystein-arginine-methionine-tyrosine- -proline-cysteine (CRMYPC) and cysteine-arginine-aspargine- phenylalanine-proline-cysteine (CRNFPC) have good residual interactions with the target and the binding energy ΔGbinding of -31.7402 and -31.0144 kcal mol-1, respectively. These values are much lower than those of the standards, and it means that both ligands are more accessible to ligand-receptor binding. Based on drug scan results, both of these ligands are neither mutagenic nor carcinogenic. They also show good oral bioavailability. Moreover, both ligands show relatively stable molecular dynamics progression of RMSD vs. time plot. However, based on our metods, the CRMYPC ligand has sufficient hydrogen bonding interactions with residues of the active side of neuraminidase-1 and can be therefore proposed as a potential inhibitor of neuraminidase-1
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spelling doaj-art-318e2ea435044aa881051f33751b2b9b2025-02-02T04:37:56ZengSciendoActa Pharmaceutica1330-00751846-95582014-06-0164215717210.2478/acph-2014-0015acph-2014-0015Computational design of drug candidates for influenza A virus subtype H1N1 by inhibiting the viral neuraminidase-1 enzymeTambunan Usman Sumo Friend0Parikesit Arli Aditya1Dephinto Yonaniko2Sipahutar Feimmy Ruth Pratiwi3Department of Chemistry Faculty of Mathematics and Sciences Depok, University of IndonesiaDepartment of Chemistry Faculty of Mathematics and Sciences Depok, University of IndonesiaDepartment of Chemistry Faculty of Mathematics and Sciences Depok, University of IndonesiaDepartment of Chemistry Faculty of Mathematics and Sciences Depok, University of IndonesiaIt is critical to seek potential alternative treatments for H1N1 infections by inhibiting neuraminidase-1 enzyme. One of the viable options for inhibiting the activity of neuraminidase- 1 is peptide drug design. In order to increase peptide stability, cyclization is necessary to prevent its digestion by protease enzyme. Cyclization of peptide ligands by formation of disulfide bridges is preferable for designing inhibitors of neuraminidase-1 because of their high activity and specificity. Here we designed ligands by using molecular docking, drug scan and dynamics computational methods. Based on our docking results, short polypeptides of cystein-arginine-methionine-tyrosine- -proline-cysteine (CRMYPC) and cysteine-arginine-aspargine- phenylalanine-proline-cysteine (CRNFPC) have good residual interactions with the target and the binding energy ΔGbinding of -31.7402 and -31.0144 kcal mol-1, respectively. These values are much lower than those of the standards, and it means that both ligands are more accessible to ligand-receptor binding. Based on drug scan results, both of these ligands are neither mutagenic nor carcinogenic. They also show good oral bioavailability. Moreover, both ligands show relatively stable molecular dynamics progression of RMSD vs. time plot. However, based on our metods, the CRMYPC ligand has sufficient hydrogen bonding interactions with residues of the active side of neuraminidase-1 and can be therefore proposed as a potential inhibitor of neuraminidase-1http://www.degruyter.com/view/j/acph.2014.64.issue-2/acph-2014-0015/acph-2014-0015.xml?format=INTH1N1neuraminidase-1cyclic peptide disulfidemolecular dockingmolecular dynamics
spellingShingle Tambunan Usman Sumo Friend
Parikesit Arli Aditya
Dephinto Yonaniko
Sipahutar Feimmy Ruth Pratiwi
Computational design of drug candidates for influenza A virus subtype H1N1 by inhibiting the viral neuraminidase-1 enzyme
Acta Pharmaceutica
H1N1
neuraminidase-1
cyclic peptide disulfide
molecular docking
molecular dynamics
title Computational design of drug candidates for influenza A virus subtype H1N1 by inhibiting the viral neuraminidase-1 enzyme
title_full Computational design of drug candidates for influenza A virus subtype H1N1 by inhibiting the viral neuraminidase-1 enzyme
title_fullStr Computational design of drug candidates for influenza A virus subtype H1N1 by inhibiting the viral neuraminidase-1 enzyme
title_full_unstemmed Computational design of drug candidates for influenza A virus subtype H1N1 by inhibiting the viral neuraminidase-1 enzyme
title_short Computational design of drug candidates for influenza A virus subtype H1N1 by inhibiting the viral neuraminidase-1 enzyme
title_sort computational design of drug candidates for influenza a virus subtype h1n1 by inhibiting the viral neuraminidase 1 enzyme
topic H1N1
neuraminidase-1
cyclic peptide disulfide
molecular docking
molecular dynamics
url http://www.degruyter.com/view/j/acph.2014.64.issue-2/acph-2014-0015/acph-2014-0015.xml?format=INT
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