Diagnostic characteristics of Xpert MTB/RIF assay for the diagnosis of tuberculous meningitis and rifampicin resistance in Southern Brazil
ABSTRACT Background: The timely diagnosis of tuberculous meningitis (TBM) is challenging. Molecular diagnostic tools are necessary for TBM, particularly in low- and middle-income countries. Objectives: We aimed to calculate the diagnostics characteristics of Xpert MTB/RIF for the detection of Myco...
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| Series: | Arquivos de Neuro-Psiquiatria |
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| Online Access: | http://www.scielo.br/pdf/anp/v78n11/1678-4227-anp-78-11-0700.pdf |
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| author | Sergio Monteiro de Almeida Gislene Maria Botão Kussen Laura Cogo José Henrique Carvalho Keite Nogueira |
| author_facet | Sergio Monteiro de Almeida Gislene Maria Botão Kussen Laura Cogo José Henrique Carvalho Keite Nogueira |
| author_sort | Sergio Monteiro de Almeida |
| collection | DOAJ |
| description | ABSTRACT Background: The timely diagnosis of tuberculous meningitis (TBM) is challenging. Molecular diagnostic tools are necessary for TBM, particularly in low- and middle-income countries. Objectives: We aimed to calculate the diagnostics characteristics of Xpert MTB/RIF for the detection of Mycobacterium tuberculosis in the cerebrospinal fluid (CSF) and the frequency of rifampicin (RIF)-resistance in the CSF samples. Methods: A total of 313 consecutive CSF samples were studied and categorized into TBM definite, probable, possible, or not TBM cases based on the clinical, laboratory, and imaging data. Results: For the definite TBM cases (n=7), the sensitivity, specificity, efficiency, and positive likelihood ratio were 100, 97, 97, and 38%, respectively. However, for the TBM definite associated with the probable cases (n=24), the sensitivity decreased to 46%. All CSF samples that were Xpert MTB/RIF-positive were RIF susceptible. Conclusion: Xpert MTB/RIF showed high discriminating value among the microbiology-proven TBM cases, although the values for the probable and possible TBM cases were reduced. Xpert MTB/RIF contributes significantly to the diagnosis of TBM, mainly when coupled with the conventional microbiological tests and clinical algorithms. |
| format | Article |
| id | doaj-art-318d4fa30c094f29b3eee8f3c708a85f |
| institution | OA Journals |
| issn | 1678-4227 |
| language | English |
| publisher | Thieme Revinter Publicações |
| record_format | Article |
| series | Arquivos de Neuro-Psiquiatria |
| spelling | doaj-art-318d4fa30c094f29b3eee8f3c708a85f2025-08-20T02:38:54ZengThieme Revinter PublicaçõesArquivos de Neuro-Psiquiatria1678-422710.1590/0004-282x20200067Diagnostic characteristics of Xpert MTB/RIF assay for the diagnosis of tuberculous meningitis and rifampicin resistance in Southern BrazilSergio Monteiro de AlmeidaGislene Maria Botão KussenLaura CogoJosé Henrique CarvalhoKeite NogueiraABSTRACT Background: The timely diagnosis of tuberculous meningitis (TBM) is challenging. Molecular diagnostic tools are necessary for TBM, particularly in low- and middle-income countries. Objectives: We aimed to calculate the diagnostics characteristics of Xpert MTB/RIF for the detection of Mycobacterium tuberculosis in the cerebrospinal fluid (CSF) and the frequency of rifampicin (RIF)-resistance in the CSF samples. Methods: A total of 313 consecutive CSF samples were studied and categorized into TBM definite, probable, possible, or not TBM cases based on the clinical, laboratory, and imaging data. Results: For the definite TBM cases (n=7), the sensitivity, specificity, efficiency, and positive likelihood ratio were 100, 97, 97, and 38%, respectively. However, for the TBM definite associated with the probable cases (n=24), the sensitivity decreased to 46%. All CSF samples that were Xpert MTB/RIF-positive were RIF susceptible. Conclusion: Xpert MTB/RIF showed high discriminating value among the microbiology-proven TBM cases, although the values for the probable and possible TBM cases were reduced. Xpert MTB/RIF contributes significantly to the diagnosis of TBM, mainly when coupled with the conventional microbiological tests and clinical algorithms.http://www.scielo.br/pdf/anp/v78n11/1678-4227-anp-78-11-0700.pdfCentral Nervous SystemDiagnosisTuberculosis, MeningealPolymerase Chain Reaction |
| spellingShingle | Sergio Monteiro de Almeida Gislene Maria Botão Kussen Laura Cogo José Henrique Carvalho Keite Nogueira Diagnostic characteristics of Xpert MTB/RIF assay for the diagnosis of tuberculous meningitis and rifampicin resistance in Southern Brazil Arquivos de Neuro-Psiquiatria Central Nervous System Diagnosis Tuberculosis, Meningeal Polymerase Chain Reaction |
| title | Diagnostic characteristics of Xpert MTB/RIF assay for the diagnosis of tuberculous meningitis and rifampicin resistance in Southern Brazil |
| title_full | Diagnostic characteristics of Xpert MTB/RIF assay for the diagnosis of tuberculous meningitis and rifampicin resistance in Southern Brazil |
| title_fullStr | Diagnostic characteristics of Xpert MTB/RIF assay for the diagnosis of tuberculous meningitis and rifampicin resistance in Southern Brazil |
| title_full_unstemmed | Diagnostic characteristics of Xpert MTB/RIF assay for the diagnosis of tuberculous meningitis and rifampicin resistance in Southern Brazil |
| title_short | Diagnostic characteristics of Xpert MTB/RIF assay for the diagnosis of tuberculous meningitis and rifampicin resistance in Southern Brazil |
| title_sort | diagnostic characteristics of xpert mtb rif assay for the diagnosis of tuberculous meningitis and rifampicin resistance in southern brazil |
| topic | Central Nervous System Diagnosis Tuberculosis, Meningeal Polymerase Chain Reaction |
| url | http://www.scielo.br/pdf/anp/v78n11/1678-4227-anp-78-11-0700.pdf |
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