The Investigation of Association Between Il-10 -1082 Polymorphism and Human Papillomavirus Infection in Prostatic Tissue Samples
Introduction: Some studies of prostate cancer (PCa) have reported the presence of human papillomavirus (HPV) DNA. Polymorphisms in IL-10 gene can influence inflammation and immune response and may be related to the risk of prostate cancer. The capacity for IL-10 production varies according to the ge...
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| Format: | Article |
| Language: | English |
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Galenos Publishing House
2012-12-01
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| Series: | Mediterranean Journal of Infection, Microbes and Antimicrobials |
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| Online Access: | http://www.mjima.org/pdf.php?&id=6 |
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| author | Ebru ETEM Yasemin BULUT Nusret AKPOLAT Yasemin AŞKIN Halit ELYAS Fügen YARKIN |
| author_facet | Ebru ETEM Yasemin BULUT Nusret AKPOLAT Yasemin AŞKIN Halit ELYAS Fügen YARKIN |
| author_sort | Ebru ETEM |
| collection | DOAJ |
| description | Introduction: Some studies of prostate cancer (PCa) have reported the presence of human papillomavirus (HPV) DNA. Polymorphisms in IL-10 gene can influence inflammation and immune response and may be related to the risk of prostate cancer. The capacity for IL-10 production varies according to the genetic composition of the IL-10 locus. We aimed to elucidate the relation between HPV infection and IL-10 polymorphism for the development of prostate cancer. We examined 108 formalin-fixed specimens for the existence of HPVs DNA and IL-10 -1082 genotype distribution.
Materials and Methods: The DNA are extracted from archival prostate tissues of totally 108 patients, 40 of whom with adenocarcinoma and 68 with benign prostatic hyperplasia (BPH). Genotypes of IL-10 -1082 single nucleotide polymorphism (SNP) were performed using allele specific polymerase chain reaction (ARMS). HPV detection was performed by using conventional HPV primers.
Results: HPVs DNA was detected in 15 of 68 BPH specimens (22%) and in 16 of 40 prostate PCa specimens (40%). Distribution of IL-10 -1082 genotype was not statistically different between PCa and BPH using chi-square (p> 0.05). There was not any association between HPVs DNA positivity and IL-10 -1082 genotypes.
Conclusion: The results suggest that the HPVs DNA positivity might be involved in the etiology of a minority of prostate cancers. As result, we consider that future investigations are needed to provide conclusive evidence on the role of this pathogen and genes in the prostate cancer. |
| format | Article |
| id | doaj-art-3186df367a9840f4aee32846002bf706 |
| institution | Kabale University |
| issn | 2147-673X |
| language | English |
| publishDate | 2012-12-01 |
| publisher | Galenos Publishing House |
| record_format | Article |
| series | Mediterranean Journal of Infection, Microbes and Antimicrobials |
| spelling | doaj-art-3186df367a9840f4aee32846002bf7062025-08-20T03:55:01ZengGalenos Publishing HouseMediterranean Journal of Infection, Microbes and Antimicrobials2147-673X2012-12-011The Investigation of Association Between Il-10 -1082 Polymorphism and Human Papillomavirus Infection in Prostatic Tissue SamplesEbru ETEM0Yasemin BULUT1Nusret AKPOLAT2Yasemin AŞKIN3Halit ELYAS4Fügen YARKIN5Fırat Üniversitesi Tıp Fakültesi, Tıbbi Biyoloji Anabilim Dalı, Elazığ, TürkiyeFırat Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, Elazığ, Türkiyeİnönü Üniversitesi Tıp Fakültesi, Turgut Özal Tıp Merkezi, Tıbbi Patoloji Anabilim Dalı, Malatya, TürkiyeFırat Üniversitesi Tıp Fakültesi, Tıbbi Biyoloji Anabilim Dalı, Elazığ, TürkiyeFırat Üniversitesi Tıp Fakültesi, Tıbbi Biyoloji Anabilim Dalı, Elazığ, TürkiyeÇukurova Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, Adana, TürkiyeIntroduction: Some studies of prostate cancer (PCa) have reported the presence of human papillomavirus (HPV) DNA. Polymorphisms in IL-10 gene can influence inflammation and immune response and may be related to the risk of prostate cancer. The capacity for IL-10 production varies according to the genetic composition of the IL-10 locus. We aimed to elucidate the relation between HPV infection and IL-10 polymorphism for the development of prostate cancer. We examined 108 formalin-fixed specimens for the existence of HPVs DNA and IL-10 -1082 genotype distribution. Materials and Methods: The DNA are extracted from archival prostate tissues of totally 108 patients, 40 of whom with adenocarcinoma and 68 with benign prostatic hyperplasia (BPH). Genotypes of IL-10 -1082 single nucleotide polymorphism (SNP) were performed using allele specific polymerase chain reaction (ARMS). HPV detection was performed by using conventional HPV primers. Results: HPVs DNA was detected in 15 of 68 BPH specimens (22%) and in 16 of 40 prostate PCa specimens (40%). Distribution of IL-10 -1082 genotype was not statistically different between PCa and BPH using chi-square (p> 0.05). There was not any association between HPVs DNA positivity and IL-10 -1082 genotypes. Conclusion: The results suggest that the HPVs DNA positivity might be involved in the etiology of a minority of prostate cancers. As result, we consider that future investigations are needed to provide conclusive evidence on the role of this pathogen and genes in the prostate cancer.http://www.mjima.org/pdf.php?&id=6Human papillomavirusprostatic cancerbenign prostatic hyperplasiainterleukin-10polymorphism |
| spellingShingle | Ebru ETEM Yasemin BULUT Nusret AKPOLAT Yasemin AŞKIN Halit ELYAS Fügen YARKIN The Investigation of Association Between Il-10 -1082 Polymorphism and Human Papillomavirus Infection in Prostatic Tissue Samples Mediterranean Journal of Infection, Microbes and Antimicrobials Human papillomavirus prostatic cancer benign prostatic hyperplasia interleukin-10 polymorphism |
| title | The Investigation of Association Between Il-10 -1082 Polymorphism and Human Papillomavirus Infection in Prostatic Tissue Samples |
| title_full | The Investigation of Association Between Il-10 -1082 Polymorphism and Human Papillomavirus Infection in Prostatic Tissue Samples |
| title_fullStr | The Investigation of Association Between Il-10 -1082 Polymorphism and Human Papillomavirus Infection in Prostatic Tissue Samples |
| title_full_unstemmed | The Investigation of Association Between Il-10 -1082 Polymorphism and Human Papillomavirus Infection in Prostatic Tissue Samples |
| title_short | The Investigation of Association Between Il-10 -1082 Polymorphism and Human Papillomavirus Infection in Prostatic Tissue Samples |
| title_sort | investigation of association between il 10 1082 polymorphism and human papillomavirus infection in prostatic tissue samples |
| topic | Human papillomavirus prostatic cancer benign prostatic hyperplasia interleukin-10 polymorphism |
| url | http://www.mjima.org/pdf.php?&id=6 |
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