GLP-1 Gene-Modified Human Umbilical Cord Mesenchymal Stem Cell Line Improves Blood Glucose Level in Type 2 Diabetic Mice
Type 2 diabetes constitutes a serious threat to the health of patients, but there is currently no ideal treatment in the clinic. Glucagon-like peptide-1 and human umbilical cord mesenchymal stem cells have been confirmed to have antidiabetic effects, but both of them have certain defects in the proc...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2019/4961865 |
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| author | Ying Chang Mingxin Dong Yan Wang Haotian Yu Chengbiao Sun Xin Jiang Wei Chen Xin Wang Na Xu Wensen Liu Ningyi Jin |
| author_facet | Ying Chang Mingxin Dong Yan Wang Haotian Yu Chengbiao Sun Xin Jiang Wei Chen Xin Wang Na Xu Wensen Liu Ningyi Jin |
| author_sort | Ying Chang |
| collection | DOAJ |
| description | Type 2 diabetes constitutes a serious threat to the health of patients, but there is currently no ideal treatment in the clinic. Glucagon-like peptide-1 and human umbilical cord mesenchymal stem cells have been confirmed to have antidiabetic effects, but both of them have certain defects in the process of antidiabetes, which cannot meet the need of clinical treatment. We hypothesized that human umbilical cord mesenchymal stem cells can be used as a vector to construct a novel cell line that expresses GLP-1 in vivo for a long time. And this cell strain results in lowering blood glucose in type 2 diabetic mice. The results showed that after 3 weeks of intramuscular injection of the new cell line, the fasting blood glucose of type 2 diabetic mice returned to the normal range, and the hypoglycemic effect was maintained within 3 weeks after putting an end to the drug. At the same time, during the administration, the mice lost weight, the food intake decreased, the half-life of GLP-1 in the body prolonged, the IR reduced, and the pancreatic function recovered. The results of this study indicate that the novel cell line can prolong the half-life of GLP-1 in vivo and effectively lower blood sugar, which is a feasible method to improve type 2 diabetes. |
| format | Article |
| id | doaj-art-318352e6e69c4e248e7ddc37fe43ff2f |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-318352e6e69c4e248e7ddc37fe43ff2f2025-02-03T05:45:39ZengWileyStem Cells International1687-966X1687-96782019-01-01201910.1155/2019/49618654961865GLP-1 Gene-Modified Human Umbilical Cord Mesenchymal Stem Cell Line Improves Blood Glucose Level in Type 2 Diabetic MiceYing Chang0Mingxin Dong1Yan Wang2Haotian Yu3Chengbiao Sun4Xin Jiang5Wei Chen6Xin Wang7Na Xu8Wensen Liu9Ningyi Jin10Medical College, Yanbian University, Yanji 133002, ChinaInstitute of Military Veterinary Medicine, Academy of Military Medical Sciences, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun 130122, ChinaInstitute of Military Veterinary Medicine, Academy of Military Medical Sciences, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun 130122, ChinaInstitute of Military Veterinary Medicine, Academy of Military Medical Sciences, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun 130122, ChinaInstitute of Military Veterinary Medicine, Academy of Military Medical Sciences, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun 130122, ChinaJilin Medical University, Jilin 132013, ChinaMedical College, Yanbian University, Yanji 133002, ChinaInstitute of Military Veterinary Medicine, Academy of Military Medical Sciences, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun 130122, ChinaInstitute of Military Veterinary Medicine, Academy of Military Medical Sciences, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun 130122, ChinaInstitute of Military Veterinary Medicine, Academy of Military Medical Sciences, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun 130122, ChinaMedical College, Yanbian University, Yanji 133002, ChinaType 2 diabetes constitutes a serious threat to the health of patients, but there is currently no ideal treatment in the clinic. Glucagon-like peptide-1 and human umbilical cord mesenchymal stem cells have been confirmed to have antidiabetic effects, but both of them have certain defects in the process of antidiabetes, which cannot meet the need of clinical treatment. We hypothesized that human umbilical cord mesenchymal stem cells can be used as a vector to construct a novel cell line that expresses GLP-1 in vivo for a long time. And this cell strain results in lowering blood glucose in type 2 diabetic mice. The results showed that after 3 weeks of intramuscular injection of the new cell line, the fasting blood glucose of type 2 diabetic mice returned to the normal range, and the hypoglycemic effect was maintained within 3 weeks after putting an end to the drug. At the same time, during the administration, the mice lost weight, the food intake decreased, the half-life of GLP-1 in the body prolonged, the IR reduced, and the pancreatic function recovered. The results of this study indicate that the novel cell line can prolong the half-life of GLP-1 in vivo and effectively lower blood sugar, which is a feasible method to improve type 2 diabetes.http://dx.doi.org/10.1155/2019/4961865 |
| spellingShingle | Ying Chang Mingxin Dong Yan Wang Haotian Yu Chengbiao Sun Xin Jiang Wei Chen Xin Wang Na Xu Wensen Liu Ningyi Jin GLP-1 Gene-Modified Human Umbilical Cord Mesenchymal Stem Cell Line Improves Blood Glucose Level in Type 2 Diabetic Mice Stem Cells International |
| title | GLP-1 Gene-Modified Human Umbilical Cord Mesenchymal Stem Cell Line Improves Blood Glucose Level in Type 2 Diabetic Mice |
| title_full | GLP-1 Gene-Modified Human Umbilical Cord Mesenchymal Stem Cell Line Improves Blood Glucose Level in Type 2 Diabetic Mice |
| title_fullStr | GLP-1 Gene-Modified Human Umbilical Cord Mesenchymal Stem Cell Line Improves Blood Glucose Level in Type 2 Diabetic Mice |
| title_full_unstemmed | GLP-1 Gene-Modified Human Umbilical Cord Mesenchymal Stem Cell Line Improves Blood Glucose Level in Type 2 Diabetic Mice |
| title_short | GLP-1 Gene-Modified Human Umbilical Cord Mesenchymal Stem Cell Line Improves Blood Glucose Level in Type 2 Diabetic Mice |
| title_sort | glp 1 gene modified human umbilical cord mesenchymal stem cell line improves blood glucose level in type 2 diabetic mice |
| url | http://dx.doi.org/10.1155/2019/4961865 |
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