Evaluating peripheral blood lymphocyte subset composition and lipopolysaccharide-induced cytokine secretory activity in mononuclear leukocyte cultures from patients with febrile and meningeal forms of tick-borne encephalitis
Introduction.Multiple studies on immune response in patients with various clinical forms of tick-borne encephalitis (TBE) have shown conflicting results. The study aim was to estimate the changes in peripheral blood lymphocyte subset counts and activity of spontaneous and lipopolysaccharide (LPS)-st...
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Sankt-Peterburg : NIIÈM imeni Pastera
2024-10-01
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| Series: | Инфекция и иммунитет |
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| Online Access: | https://iimmun.ru/iimm/article/viewFile/17647/2081 |
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| author | Ekaterina N. Ilyinskikh O. V. Voronkova A. V. Reshetova R. R. Hasanova I. E. Esimova N. A. Chernyshov O. V. Yampolskaya A. V. Yampolskaya E. M. Polomoshnova E. V. Minakova |
| author_facet | Ekaterina N. Ilyinskikh O. V. Voronkova A. V. Reshetova R. R. Hasanova I. E. Esimova N. A. Chernyshov O. V. Yampolskaya A. V. Yampolskaya E. M. Polomoshnova E. V. Minakova |
| author_sort | Ekaterina N. Ilyinskikh |
| collection | DOAJ |
| description | Introduction.Multiple studies on immune response in patients with various clinical forms of tick-borne encephalitis (TBE) have shown conflicting results. The study aim was to estimate the changes in peripheral blood lymphocyte subset counts and activity of spontaneous and lipopolysaccharide (LPS)-stimulated cytokine production in the mononuclear leukocyte cultures in patients with febrile and meningeal acute TBE.Materials and methods.Groups 1 and 2 included 16 and 12 patients with febrile and meningeal acute TBE, respectively. The control group included 13 healthy donors. Hemogram and T-lymphocyte, T-helper cell, T-cytotoxic and NK cell counts were analyzed by flow cytometry at week 1 of the disease as well as the spontaneous and LPS-stimulated secretion levels of TNFα, IL-1β, IL-6, IL-10, IL-8, and MCP-1 were assessed by ELISA in the supernatants of mononuclear cell cultures twice: during hospitalization and two weeks later. Statistical analysis was performed by the Mann–Whitney U-test, and Wilcoxon test.Results.Group 2 demonstrated significant decrease in spontaneous and/or LPS-stimulated levels of proinflammatory cytokines IL-1β, IL-6, MCP-1, and TNFα, but showed higher IL-8 level as compared with Group 1. In addition, spontaneous and/or LPS-induced levels of IL-6 and TNFαin Group 2 did not significantly differ from the controls, which presumably also indicated the suppression of their production. In contrast, spontaneous and/or LPS-induced levels of IL-1β, IL-6, MCP-1, and TNFαin Group 1 were higher than in the controls. The spontaneous IL-10 level in the patients from both groups were higher than in the controls. Peripheral blood lymphocyte and T-cytotoxic T-lymphocyte counts in both groups of TBE patients were lower than in the controls. There was significant increase in neutrophil counts, decrease in NK cell count in Group 2 as compared to the patients with a milder febrile form.Conclusion.Meningeal acute TBE patients was presumably associated with inadequate immune response with NK cell deficiency, T-cell dysfunction, increased neutrophil count in the peripheral blood and impaired pro-inflammatory cytokine production related to innate immune response. |
| format | Article |
| id | doaj-art-3173d19e0c8d4e1bbafe2a4a7ef59380 |
| institution | OA Journals |
| issn | 2220-7619 2313-7398 |
| language | Russian |
| publishDate | 2024-10-01 |
| publisher | Sankt-Peterburg : NIIÈM imeni Pastera |
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| series | Инфекция и иммунитет |
| spelling | doaj-art-3173d19e0c8d4e1bbafe2a4a7ef593802025-08-20T02:26:19ZrusSankt-Peterburg : NIIÈM imeni PasteraИнфекция и иммунитет2220-76192313-73982024-10-0114469070010.15789/2220-7619-EPB-176471441Evaluating peripheral blood lymphocyte subset composition and lipopolysaccharide-induced cytokine secretory activity in mononuclear leukocyte cultures from patients with febrile and meningeal forms of tick-borne encephalitisEkaterina N. Ilyinskikh0O. V. Voronkova1A. V. Reshetova2R. R. Hasanova3I. E. Esimova4N. A. Chernyshov5O. V. Yampolskaya6A. V. Yampolskaya7E. M. Polomoshnova8E. V. Minakova9Siberian State Medical UniversitySiberian State Medical UniversitySiberian State Medical UniversitySiberian State Medical UniversitySiberian State Medical UniversitySiberian State Medical UniversitySiberian State Medical UniversitySiberian State Medical UniversitySiberian State Medical UniversitySiberian State Medical UniversityIntroduction.Multiple studies on immune response in patients with various clinical forms of tick-borne encephalitis (TBE) have shown conflicting results. The study aim was to estimate the changes in peripheral blood lymphocyte subset counts and activity of spontaneous and lipopolysaccharide (LPS)-stimulated cytokine production in the mononuclear leukocyte cultures in patients with febrile and meningeal acute TBE.Materials and methods.Groups 1 and 2 included 16 and 12 patients with febrile and meningeal acute TBE, respectively. The control group included 13 healthy donors. Hemogram and T-lymphocyte, T-helper cell, T-cytotoxic and NK cell counts were analyzed by flow cytometry at week 1 of the disease as well as the spontaneous and LPS-stimulated secretion levels of TNFα, IL-1β, IL-6, IL-10, IL-8, and MCP-1 were assessed by ELISA in the supernatants of mononuclear cell cultures twice: during hospitalization and two weeks later. Statistical analysis was performed by the Mann–Whitney U-test, and Wilcoxon test.Results.Group 2 demonstrated significant decrease in spontaneous and/or LPS-stimulated levels of proinflammatory cytokines IL-1β, IL-6, MCP-1, and TNFα, but showed higher IL-8 level as compared with Group 1. In addition, spontaneous and/or LPS-induced levels of IL-6 and TNFαin Group 2 did not significantly differ from the controls, which presumably also indicated the suppression of their production. In contrast, spontaneous and/or LPS-induced levels of IL-1β, IL-6, MCP-1, and TNFαin Group 1 were higher than in the controls. The spontaneous IL-10 level in the patients from both groups were higher than in the controls. Peripheral blood lymphocyte and T-cytotoxic T-lymphocyte counts in both groups of TBE patients were lower than in the controls. There was significant increase in neutrophil counts, decrease in NK cell count in Group 2 as compared to the patients with a milder febrile form.Conclusion.Meningeal acute TBE patients was presumably associated with inadequate immune response with NK cell deficiency, T-cell dysfunction, increased neutrophil count in the peripheral blood and impaired pro-inflammatory cytokine production related to innate immune response.https://iimmun.ru/iimm/article/viewFile/17647/2081tick-borne encephalitisfebrile formmeningeal formcytokineslymphocyte subpopulationslipopolysaccharidemononuclear blood leukocytes |
| spellingShingle | Ekaterina N. Ilyinskikh O. V. Voronkova A. V. Reshetova R. R. Hasanova I. E. Esimova N. A. Chernyshov O. V. Yampolskaya A. V. Yampolskaya E. M. Polomoshnova E. V. Minakova Evaluating peripheral blood lymphocyte subset composition and lipopolysaccharide-induced cytokine secretory activity in mononuclear leukocyte cultures from patients with febrile and meningeal forms of tick-borne encephalitis Инфекция и иммунитет tick-borne encephalitis febrile form meningeal form cytokines lymphocyte subpopulations lipopolysaccharide mononuclear blood leukocytes |
| title | Evaluating peripheral blood lymphocyte subset composition and lipopolysaccharide-induced cytokine secretory activity in mononuclear leukocyte cultures from patients with febrile and meningeal forms of tick-borne encephalitis |
| title_full | Evaluating peripheral blood lymphocyte subset composition and lipopolysaccharide-induced cytokine secretory activity in mononuclear leukocyte cultures from patients with febrile and meningeal forms of tick-borne encephalitis |
| title_fullStr | Evaluating peripheral blood lymphocyte subset composition and lipopolysaccharide-induced cytokine secretory activity in mononuclear leukocyte cultures from patients with febrile and meningeal forms of tick-borne encephalitis |
| title_full_unstemmed | Evaluating peripheral blood lymphocyte subset composition and lipopolysaccharide-induced cytokine secretory activity in mononuclear leukocyte cultures from patients with febrile and meningeal forms of tick-borne encephalitis |
| title_short | Evaluating peripheral blood lymphocyte subset composition and lipopolysaccharide-induced cytokine secretory activity in mononuclear leukocyte cultures from patients with febrile and meningeal forms of tick-borne encephalitis |
| title_sort | evaluating peripheral blood lymphocyte subset composition and lipopolysaccharide induced cytokine secretory activity in mononuclear leukocyte cultures from patients with febrile and meningeal forms of tick borne encephalitis |
| topic | tick-borne encephalitis febrile form meningeal form cytokines lymphocyte subpopulations lipopolysaccharide mononuclear blood leukocytes |
| url | https://iimmun.ru/iimm/article/viewFile/17647/2081 |
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