Huaier Cream Protects against Adriamycin-Induced Nephropathy by Restoring Mitochondrial Function via PGC-1α Upregulation
The mechanism by which Huaier, a Chinese traditional medicine, protects podocytes remains unclear. We designed the present study to examine whether mitochondrial function restored by PGC-1α serves as the major target of Huaier cream in protecting ADR nephropathy. After ADR administration, the podocy...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | PPAR Research |
| Online Access: | http://dx.doi.org/10.1155/2015/720383 |
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| author | Ruochen Che Chunhua Zhu Guixia Ding Min Zhao Mi Bai Zhanjun Jia Aihua Zhang Songming Huang |
| author_facet | Ruochen Che Chunhua Zhu Guixia Ding Min Zhao Mi Bai Zhanjun Jia Aihua Zhang Songming Huang |
| author_sort | Ruochen Che |
| collection | DOAJ |
| description | The mechanism by which Huaier, a Chinese traditional medicine, protects podocytes remains unclear. We designed the present study to examine whether mitochondrial function restored by PGC-1α serves as the major target of Huaier cream in protecting ADR nephropathy. After ADR administration, the podocytes exhibited remarkable cell injury and mitochondrial dysfunction. Additionally, ADR also reduced PGC-1α both in vivo and in vitro. Following the Huaier treatment, the notable downregulation of PGC-1α and its downstream molecule mitochondrial transcription factor A (TFAM) were almost entirely blocked. Correspondingly, Huaier markedly ameliorated ADR-induced podocyte injury and mitochondrial dysfunction in both rat kidneys and incubated cells as it inhibited the decrease of nephrin and podocin expression, mtDNA copy number, MMP, and ATP content. Transmission electron microscopy result also showed that Huaier protected mitochondria against ADR-induced severe mitophagy and abnormal changes of ultrastructural morphology. In conclusion, Huaier can protect podocytes against ADR-induced cytotoxicity possibly by reversing the dysfunction of mitochondria via PGC-1α overexpression, which may be a novel therapeutic drug target in glomerular diseases. |
| format | Article |
| id | doaj-art-3168730636ff488bb28371089bdb26cf |
| institution | Kabale University |
| issn | 1687-4757 1687-4765 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | PPAR Research |
| spelling | doaj-art-3168730636ff488bb28371089bdb26cf2025-08-20T03:55:01ZengWileyPPAR Research1687-47571687-47652015-01-01201510.1155/2015/720383720383Huaier Cream Protects against Adriamycin-Induced Nephropathy by Restoring Mitochondrial Function via PGC-1α UpregulationRuochen Che0Chunhua Zhu1Guixia Ding2Min Zhao3Mi Bai4Zhanjun Jia5Aihua Zhang6Songming Huang7Department of Nephrology, Nanjing Children’s Hospital, Nanjing Medical University, 72 Guangzhou Road, Nanjing, Jiangsu 210029, ChinaDepartment of Nephrology, Nanjing Children’s Hospital, Nanjing Medical University, 72 Guangzhou Road, Nanjing, Jiangsu 210029, ChinaDepartment of Nephrology, Nanjing Children’s Hospital, Nanjing Medical University, 72 Guangzhou Road, Nanjing, Jiangsu 210029, ChinaDepartment of Nephrology, Nanjing Children’s Hospital, Nanjing Medical University, 72 Guangzhou Road, Nanjing, Jiangsu 210029, ChinaDepartment of Nephrology, Nanjing Children’s Hospital, Nanjing Medical University, 72 Guangzhou Road, Nanjing, Jiangsu 210029, ChinaDepartment of Nephrology, Nanjing Children’s Hospital, Nanjing Medical University, 72 Guangzhou Road, Nanjing, Jiangsu 210029, ChinaDepartment of Nephrology, Nanjing Children’s Hospital, Nanjing Medical University, 72 Guangzhou Road, Nanjing, Jiangsu 210029, ChinaDepartment of Nephrology, Nanjing Children’s Hospital, Nanjing Medical University, 72 Guangzhou Road, Nanjing, Jiangsu 210029, ChinaThe mechanism by which Huaier, a Chinese traditional medicine, protects podocytes remains unclear. We designed the present study to examine whether mitochondrial function restored by PGC-1α serves as the major target of Huaier cream in protecting ADR nephropathy. After ADR administration, the podocytes exhibited remarkable cell injury and mitochondrial dysfunction. Additionally, ADR also reduced PGC-1α both in vivo and in vitro. Following the Huaier treatment, the notable downregulation of PGC-1α and its downstream molecule mitochondrial transcription factor A (TFAM) were almost entirely blocked. Correspondingly, Huaier markedly ameliorated ADR-induced podocyte injury and mitochondrial dysfunction in both rat kidneys and incubated cells as it inhibited the decrease of nephrin and podocin expression, mtDNA copy number, MMP, and ATP content. Transmission electron microscopy result also showed that Huaier protected mitochondria against ADR-induced severe mitophagy and abnormal changes of ultrastructural morphology. In conclusion, Huaier can protect podocytes against ADR-induced cytotoxicity possibly by reversing the dysfunction of mitochondria via PGC-1α overexpression, which may be a novel therapeutic drug target in glomerular diseases.http://dx.doi.org/10.1155/2015/720383 |
| spellingShingle | Ruochen Che Chunhua Zhu Guixia Ding Min Zhao Mi Bai Zhanjun Jia Aihua Zhang Songming Huang Huaier Cream Protects against Adriamycin-Induced Nephropathy by Restoring Mitochondrial Function via PGC-1α Upregulation PPAR Research |
| title | Huaier Cream Protects against Adriamycin-Induced Nephropathy by Restoring Mitochondrial Function via PGC-1α Upregulation |
| title_full | Huaier Cream Protects against Adriamycin-Induced Nephropathy by Restoring Mitochondrial Function via PGC-1α Upregulation |
| title_fullStr | Huaier Cream Protects against Adriamycin-Induced Nephropathy by Restoring Mitochondrial Function via PGC-1α Upregulation |
| title_full_unstemmed | Huaier Cream Protects against Adriamycin-Induced Nephropathy by Restoring Mitochondrial Function via PGC-1α Upregulation |
| title_short | Huaier Cream Protects against Adriamycin-Induced Nephropathy by Restoring Mitochondrial Function via PGC-1α Upregulation |
| title_sort | huaier cream protects against adriamycin induced nephropathy by restoring mitochondrial function via pgc 1α upregulation |
| url | http://dx.doi.org/10.1155/2015/720383 |
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