Development of a simple allele-specific PCR for the detection of pathogenic Vibrio cholerae O1 and O139 in seafood

This study developed a simple allele specific (AS) PCR assay for the detection of pathogenic Vibrio cholerae strains in seafood. A pair of primers, (1) an AS primer targeting the mutation at site G450 in the hapR2 allele and a (2) common forward primer, were designed to detect the pathogenic O1 and...

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Bibliographic Details
Main Authors: Jant Cres Caigoy, Toshi Shimamoto, Tadashi Shimamoto
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:Journal of Agriculture and Food Research
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666154324004952
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