Development of a simple allele-specific PCR for the detection of pathogenic Vibrio cholerae O1 and O139 in seafood
This study developed a simple allele specific (AS) PCR assay for the detection of pathogenic Vibrio cholerae strains in seafood. A pair of primers, (1) an AS primer targeting the mutation at site G450 in the hapR2 allele and a (2) common forward primer, were designed to detect the pathogenic O1 and...
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| Main Authors: | Jant Cres Caigoy, Toshi Shimamoto, Tadashi Shimamoto |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-12-01
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| Series: | Journal of Agriculture and Food Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666154324004952 |
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