Methicillin-resistant staphylococcus aureus in milk from dairy cows with chronic mastitis
<b>Aim:</b> The aims of this study were: i) to isolate coagulase positive Staphylococcus (CPS) and coagulase-negative Staphylococcus (CNS) in milk from dairy cows with chronic recurrent mastitis, ii) to verify isolates in terms of Staphylococcus spp., Staphylococcus aureus and methicilli...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Selcuk University Press
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| Series: | Eurasian Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1188 |
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| Summary: | <b>Aim:</b> The aims of this study were: i) to isolate coagulase
positive Staphylococcus (CPS) and coagulase-negative Staphylococcus
(CNS) in milk from dairy cows with chronic recurrent
mastitis, ii) to verify isolates in terms of Staphylococcus spp.,
Staphylococcus aureus and methicillin-resistant Staphylococcus
aureus (MRSA) by multiplex PCR, and iii) to determine the antimicrobial
susceptibility of the isolates by disk diffusion method.<p>
<b>Materials and Methods:</b> A total of 52 milk samples were collected
from the dairy cows with chronic recurrent mastitis in
Hatay province. Isolation and identification of CPS and CNS
were detected by using classical culture method. All CPS and
CNS isolates were investigated for antimicrobial susceptibility
and also, were confirmed as Staphylococcus spp., S. aureus and
MRSA by multiplex PCR based on 16S rRNA, nuc, and mecA gene
sequences, respectively.<p>
<b>Results:</b> Positive results were found in 48.0% of the milk
samp-les. A total of 56 Staphylococcus spp. isolates (22 CPS
and 34 CNS) were obtained from the positive samples. Twenty
(90.9%) of the CPS isolates were detected as S. aureus. The
mecA gene was detected in 18 (90%) of the S. aureus isolates.
By disk dif-fusion method, resistance to erythromycin
(66%), tetracycli-ne (62.5%), ampicillin (60.7%), penicillin
(60.7%), cefoxitin (50%), oxacillin (46.4%), and
chloramphenicol (5.3%) was also determined in this study.<p>
<b>Conclusion:</b> In the study, the detection of antibiotic resistant
Staphylococcus strains and especially MRSA in milk from dairy
cows with chronic recurrent mastitis should be taken into account
for potential spread through the dairy food chain. |
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| ISSN: | 1309-6958 2146-1953 |