Toll-Like Receptor Homolog CD180 Expression Is Diminished on Natural Autoantibody-Producing B Cells of Patients with Autoimmune CNS Disorders
Purpose. Decreased expression of TLR homolog CD180 in peripheral blood B cells and its potential role in antibody production have been described in autoimmune diseases. Effectiveness of anti-CD20 therapy in neuromyelitis optica spectrum disorder (NMOSD) and multiple sclerosis (MS) strengthens the ro...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2021/9953317 |
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| author | Zsófia Hayden Szabina Erdő-Bonyár Beáta Bóné Noémi Balázs Kornélia Bodó Zsolt Illes Timea Berki Diána Simon |
| author_facet | Zsófia Hayden Szabina Erdő-Bonyár Beáta Bóné Noémi Balázs Kornélia Bodó Zsolt Illes Timea Berki Diána Simon |
| author_sort | Zsófia Hayden |
| collection | DOAJ |
| description | Purpose. Decreased expression of TLR homolog CD180 in peripheral blood B cells and its potential role in antibody production have been described in autoimmune diseases. Effectiveness of anti-CD20 therapy in neuromyelitis optica spectrum disorder (NMOSD) and multiple sclerosis (MS) strengthens the role of B cells in the pathogenesis. Therefore, we aimed to investigate the CD180 expression of peripheral blood B cell subsets in NMOSD and MS patients and analyze the levels of natural anti-citrate synthase (CS) IgG autoantibodies and IgG antibodies induced by bacterial infections reported to play a role in the pathogenesis of NMOSD or MS. Methods. We analyzed the distribution and CD180 expression of peripheral blood B cell subsets, defined by CD19/CD27/IgD staining, and measured anti-CS IgM/G natural autoantibody and antibacterial IgG serum levels in NMOSD, RRMS, and healthy controls (HC). Results. We found decreased naïve and increased memory B cells in NMOSD compared to MS. Among the investigated four B cell subsets, CD180 expression was exclusively decreased in CD19+CD27+IgD+ nonswitched (NS) memory B cells in both NMOSD and MS compared to HC. Furthermore, the anti-CS IgM natural autoantibody serum level was lower in both NMOSD and MS. In addition, we found a tendency of higher anti-CS IgG natural autoantibody levels only in anti-Chlamydia IgG antibody-positive NMOSD and MS patients. Conclusions. Our results suggest that reduced CD180 expression of NS B cells could contribute to the deficient natural IgM autoantibody production in NMOSD and MS, whereas natural IgG autoantibody levels show an association with antibacterial antibodies. |
| format | Article |
| id | doaj-art-313a4900313045049a333a34e0af45e9 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-313a4900313045049a333a34e0af45e92025-02-03T01:10:53ZengWileyJournal of Immunology Research2314-88612314-71562021-01-01202110.1155/2021/99533179953317Toll-Like Receptor Homolog CD180 Expression Is Diminished on Natural Autoantibody-Producing B Cells of Patients with Autoimmune CNS DisordersZsófia Hayden0Szabina Erdő-Bonyár1Beáta Bóné2Noémi Balázs3Kornélia Bodó4Zsolt Illes5Timea Berki6Diána Simon7Department of Immunology and Biotechnology, Clinical Center, University of Pécs Medical School, H-7624 Pécs, HungaryDepartment of Immunology and Biotechnology, Clinical Center, University of Pécs Medical School, H-7624 Pécs, HungaryDepartment of Neurology, Clinical Center, University of Pécs Medical School, H-7623 Pécs, HungaryDepartment of Immunology and Biotechnology, Clinical Center, University of Pécs Medical School, H-7624 Pécs, HungaryDepartment of Immunology and Biotechnology, Clinical Center, University of Pécs Medical School, H-7624 Pécs, HungaryDepartment of Neurology, Odense University Hospital, DenmarkDepartment of Immunology and Biotechnology, Clinical Center, University of Pécs Medical School, H-7624 Pécs, HungaryDepartment of Immunology and Biotechnology, Clinical Center, University of Pécs Medical School, H-7624 Pécs, HungaryPurpose. Decreased expression of TLR homolog CD180 in peripheral blood B cells and its potential role in antibody production have been described in autoimmune diseases. Effectiveness of anti-CD20 therapy in neuromyelitis optica spectrum disorder (NMOSD) and multiple sclerosis (MS) strengthens the role of B cells in the pathogenesis. Therefore, we aimed to investigate the CD180 expression of peripheral blood B cell subsets in NMOSD and MS patients and analyze the levels of natural anti-citrate synthase (CS) IgG autoantibodies and IgG antibodies induced by bacterial infections reported to play a role in the pathogenesis of NMOSD or MS. Methods. We analyzed the distribution and CD180 expression of peripheral blood B cell subsets, defined by CD19/CD27/IgD staining, and measured anti-CS IgM/G natural autoantibody and antibacterial IgG serum levels in NMOSD, RRMS, and healthy controls (HC). Results. We found decreased naïve and increased memory B cells in NMOSD compared to MS. Among the investigated four B cell subsets, CD180 expression was exclusively decreased in CD19+CD27+IgD+ nonswitched (NS) memory B cells in both NMOSD and MS compared to HC. Furthermore, the anti-CS IgM natural autoantibody serum level was lower in both NMOSD and MS. In addition, we found a tendency of higher anti-CS IgG natural autoantibody levels only in anti-Chlamydia IgG antibody-positive NMOSD and MS patients. Conclusions. Our results suggest that reduced CD180 expression of NS B cells could contribute to the deficient natural IgM autoantibody production in NMOSD and MS, whereas natural IgG autoantibody levels show an association with antibacterial antibodies.http://dx.doi.org/10.1155/2021/9953317 |
| spellingShingle | Zsófia Hayden Szabina Erdő-Bonyár Beáta Bóné Noémi Balázs Kornélia Bodó Zsolt Illes Timea Berki Diána Simon Toll-Like Receptor Homolog CD180 Expression Is Diminished on Natural Autoantibody-Producing B Cells of Patients with Autoimmune CNS Disorders Journal of Immunology Research |
| title | Toll-Like Receptor Homolog CD180 Expression Is Diminished on Natural Autoantibody-Producing B Cells of Patients with Autoimmune CNS Disorders |
| title_full | Toll-Like Receptor Homolog CD180 Expression Is Diminished on Natural Autoantibody-Producing B Cells of Patients with Autoimmune CNS Disorders |
| title_fullStr | Toll-Like Receptor Homolog CD180 Expression Is Diminished on Natural Autoantibody-Producing B Cells of Patients with Autoimmune CNS Disorders |
| title_full_unstemmed | Toll-Like Receptor Homolog CD180 Expression Is Diminished on Natural Autoantibody-Producing B Cells of Patients with Autoimmune CNS Disorders |
| title_short | Toll-Like Receptor Homolog CD180 Expression Is Diminished on Natural Autoantibody-Producing B Cells of Patients with Autoimmune CNS Disorders |
| title_sort | toll like receptor homolog cd180 expression is diminished on natural autoantibody producing b cells of patients with autoimmune cns disorders |
| url | http://dx.doi.org/10.1155/2021/9953317 |
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