Immunohistochemical characteristics of epithelial-mesenchymal transition in pancreatic ductal adenocarcinoma
The epithelial-mesenchymal transition (EMT) plays a crucial role in the development of pancreatic ductal adenocarcinoma (PDAC). There are insufficient and contradictory data in the scientific literature on the peculiarities of epithelial and mesenchymal marker expression in PDAC ducts and EMT zone,...
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Zaporizhzhia State Medical and Pharmaceutical University
2024-04-01
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| Series: | Zaporožskij Medicinskij Žurnal |
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| Online Access: | http://zmj.zsmu.edu.ua/article/view/297243/293359 |
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| author | M. A. Shyshkin V. O. Kabachenko |
| author_facet | M. A. Shyshkin V. O. Kabachenko |
| author_sort | M. A. Shyshkin |
| collection | DOAJ |
| description | The epithelial-mesenchymal transition (EMT) plays a crucial role in the development of pancreatic ductal adenocarcinoma (PDAC). There are insufficient and contradictory data in the scientific literature on the peculiarities of epithelial and mesenchymal marker expression in PDAC ducts and EMT zone, which requires further research on the role of EMT in the development and progression of PDAC.
Aim: to conduct a comprehensive assessment of epithelial and mesenchymal markers of EMT in the PDAC ducts and EMT zone at different degrees of differentiation.
Materials and methods. A comprehensive pathomorphological and immunohistochemical examination including 49 cases of surgical material from patients with PDAC, divided into groups of moderate (G2) and low degree of tumor differentiation (G3).
Results. PDAC was characterized by low levels of E-cadherin expression in both ducts and EMT – Me = 22.58 % [12.81; 36.23] and Me = 25.17 % [19.04; 35.37], respectively (p > 0.05). Only membrane expression of the marker was detected in the ducts and membrane-cytoplasmic one – in the EMT zone without significant differences in the groups. There was a significant decrease in the ductal β-catenin expression (p < 0.05) in G2 with membrane-cytoplasmic staining (Me = 15.58 % [10.42; 26.24]), in G3 – with membrane (Me = 4.42 % [2.35; 5.93]); in the EMT zone, only membrane-cytoplasmic expression was observed in both groups without significant difference (p > 0.05). Membrane expression of CK7 was positive in 100 % of PDAC, significantly lower at G2 (Me = 19.51 % [10.70; 27.24]; Me = 26.19 % [20.93; 30.05], p < 0.05), and CK18 (Me = 21.34 % [9.68; 29.96] – in G2, in G3 – Me = 22.50 % [8.24; 40.08], p > 0.05). Expression of α-SMA and Vim was seen in spindle-shaped stromal cells, around tubular and trabecular structures with membrane-cytoplasmic staining. There was no significant difference between α-SMA in G2 and G3, the level of vimentin was significantly lower in G3 (p < 0.05).
Conclusions. The features of PDAC E-cadherin, β-catenin, CK7 and CK18 marker expression indicate a greater EMT process at the periphery of the tumor and its severity increases with tumor progression. The optimal markers to determine the mesenchymal phenotype of PDAC cells are α-SMA and vimentin. |
| format | Article |
| id | doaj-art-3138f383cb614e7998026e883fcea2ae |
| institution | OA Journals |
| issn | 2306-4145 2310-1210 |
| language | English |
| publishDate | 2024-04-01 |
| publisher | Zaporizhzhia State Medical and Pharmaceutical University |
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| series | Zaporožskij Medicinskij Žurnal |
| spelling | doaj-art-3138f383cb614e7998026e883fcea2ae2025-08-20T02:01:35ZengZaporizhzhia State Medical and Pharmaceutical UniversityZaporožskij Medicinskij Žurnal2306-41452310-12102024-04-0126212713310.14739/2310-1210.2024.2.297243Immunohistochemical characteristics of epithelial-mesenchymal transition in pancreatic ductal adenocarcinomaM. A. Shyshkin0https://orcid.org/0000-0002-8979-8463V. O. Kabachenko1https://orcid.org/0000-0002-7219-6634Zaporizhzhia State Medical and Pharmaceutical University, UkraineZaporizhzhia State Medical and Pharmaceutical University, UkraineThe epithelial-mesenchymal transition (EMT) plays a crucial role in the development of pancreatic ductal adenocarcinoma (PDAC). There are insufficient and contradictory data in the scientific literature on the peculiarities of epithelial and mesenchymal marker expression in PDAC ducts and EMT zone, which requires further research on the role of EMT in the development and progression of PDAC. Aim: to conduct a comprehensive assessment of epithelial and mesenchymal markers of EMT in the PDAC ducts and EMT zone at different degrees of differentiation. Materials and methods. A comprehensive pathomorphological and immunohistochemical examination including 49 cases of surgical material from patients with PDAC, divided into groups of moderate (G2) and low degree of tumor differentiation (G3). Results. PDAC was characterized by low levels of E-cadherin expression in both ducts and EMT – Me = 22.58 % [12.81; 36.23] and Me = 25.17 % [19.04; 35.37], respectively (p > 0.05). Only membrane expression of the marker was detected in the ducts and membrane-cytoplasmic one – in the EMT zone without significant differences in the groups. There was a significant decrease in the ductal β-catenin expression (p < 0.05) in G2 with membrane-cytoplasmic staining (Me = 15.58 % [10.42; 26.24]), in G3 – with membrane (Me = 4.42 % [2.35; 5.93]); in the EMT zone, only membrane-cytoplasmic expression was observed in both groups without significant difference (p > 0.05). Membrane expression of CK7 was positive in 100 % of PDAC, significantly lower at G2 (Me = 19.51 % [10.70; 27.24]; Me = 26.19 % [20.93; 30.05], p < 0.05), and CK18 (Me = 21.34 % [9.68; 29.96] – in G2, in G3 – Me = 22.50 % [8.24; 40.08], p > 0.05). Expression of α-SMA and Vim was seen in spindle-shaped stromal cells, around tubular and trabecular structures with membrane-cytoplasmic staining. There was no significant difference between α-SMA in G2 and G3, the level of vimentin was significantly lower in G3 (p < 0.05). Conclusions. The features of PDAC E-cadherin, β-catenin, CK7 and CK18 marker expression indicate a greater EMT process at the periphery of the tumor and its severity increases with tumor progression. The optimal markers to determine the mesenchymal phenotype of PDAC cells are α-SMA and vimentin.http://zmj.zsmu.edu.ua/article/view/297243/293359pancreatic ductal adenocarcinomapancreasepithelial to mesenchymal transition (emt)immunohistochemistrye-cadherinbeta-cateninalpha-smavimentincytokeratin 7 (ck7)cytokeratin 18 (ck18) |
| spellingShingle | M. A. Shyshkin V. O. Kabachenko Immunohistochemical characteristics of epithelial-mesenchymal transition in pancreatic ductal adenocarcinoma Zaporožskij Medicinskij Žurnal pancreatic ductal adenocarcinoma pancreas epithelial to mesenchymal transition (emt) immunohistochemistry e-cadherin beta-catenin alpha-sma vimentin cytokeratin 7 (ck7) cytokeratin 18 (ck18) |
| title | Immunohistochemical characteristics of epithelial-mesenchymal transition in pancreatic ductal adenocarcinoma |
| title_full | Immunohistochemical characteristics of epithelial-mesenchymal transition in pancreatic ductal adenocarcinoma |
| title_fullStr | Immunohistochemical characteristics of epithelial-mesenchymal transition in pancreatic ductal adenocarcinoma |
| title_full_unstemmed | Immunohistochemical characteristics of epithelial-mesenchymal transition in pancreatic ductal adenocarcinoma |
| title_short | Immunohistochemical characteristics of epithelial-mesenchymal transition in pancreatic ductal adenocarcinoma |
| title_sort | immunohistochemical characteristics of epithelial mesenchymal transition in pancreatic ductal adenocarcinoma |
| topic | pancreatic ductal adenocarcinoma pancreas epithelial to mesenchymal transition (emt) immunohistochemistry e-cadherin beta-catenin alpha-sma vimentin cytokeratin 7 (ck7) cytokeratin 18 (ck18) |
| url | http://zmj.zsmu.edu.ua/article/view/297243/293359 |
| work_keys_str_mv | AT mashyshkin immunohistochemicalcharacteristicsofepithelialmesenchymaltransitioninpancreaticductaladenocarcinoma AT vokabachenko immunohistochemicalcharacteristicsofepithelialmesenchymaltransitioninpancreaticductaladenocarcinoma |