Microbiome within Primary Tumor Tissue from Renal Cell Carcinoma May Be Associated with PD-L1 Expression of the Venous Tumor Thrombus
Objective. To perform a proof of concept microbiome evaluation and PD-L1 expression profiling in clear-cell renal cell carcinoma (cc-RCC) with associated tumor thrombus (TT). Methods. After IRB approval, six patients underwent radical nephrectomy (RN) with venous tumor thrombectomy (VTT). We collect...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Advances in Urology |
| Online Access: | http://dx.doi.org/10.1155/2020/9068068 |
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| author | Michael A. Liss Yidong Chen Ronald Rodriguez Deepak Pruthi Teresa Johnson-Pais Hanzhang Wang Ahmed Mansour James R. White Dharam Kaushik |
| author_facet | Michael A. Liss Yidong Chen Ronald Rodriguez Deepak Pruthi Teresa Johnson-Pais Hanzhang Wang Ahmed Mansour James R. White Dharam Kaushik |
| author_sort | Michael A. Liss |
| collection | DOAJ |
| description | Objective. To perform a proof of concept microbiome evaluation and PD-L1 expression profiling in clear-cell renal cell carcinoma (cc-RCC) with associated tumor thrombus (TT). Methods. After IRB approval, six patients underwent radical nephrectomy (RN) with venous tumor thrombectomy (VTT). We collected fresh tissue specimens from normal adjacent, tumor, and thrombus tissues. We utilized RNA sequencing to obtain PD-L1 expression profiles and perform microbiome analysis. Statistical assessment was performed using Student’s t-test, chi-square, and spearman rank correlations using SPSS v25. Results. We noted the tumor thrombus to be mostly devoid of diverse microbiota. A large proportion of Staphylococcus epidermidus was detected and unknown if this is a surgical or postsurgical contaminant; however, it was noted more in the thrombus than other tissues. Microbiome diversity profiles were most abundant in the primary tumor compared to the thrombus or normal adjacent tissue. Differential expression of PD-L1 was examined in the tumor thrombus to the normal background tissue and noted three of the six subjects had a threshold above 2-fold. These three similar subjects had foreign microbiota that are typical residents of the oral microbiome. Conclusion. Renal tumors have more diverse microbiomes than normal adjacent tissue. Identification of resident oral microbiome profiles in clear-cell renal cancer with tumor thrombus provides a potential biomarker for thrombus response to PD-L1 inhibition. |
| format | Article |
| id | doaj-art-313197fe3c2049c19b7cf4eb11b6ef9b |
| institution | OA Journals |
| issn | 1687-6369 1687-6377 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Urology |
| spelling | doaj-art-313197fe3c2049c19b7cf4eb11b6ef9b2025-08-20T02:38:53ZengWileyAdvances in Urology1687-63691687-63772020-01-01202010.1155/2020/90680689068068Microbiome within Primary Tumor Tissue from Renal Cell Carcinoma May Be Associated with PD-L1 Expression of the Venous Tumor ThrombusMichael A. Liss0Yidong Chen1Ronald Rodriguez2Deepak Pruthi3Teresa Johnson-Pais4Hanzhang Wang5Ahmed Mansour6James R. White7Dharam Kaushik8Department of Urology, University of Texas Health San Antonio, San Antonio, TX, USAGreehey Children’s Cancer Research Institute, University of Texas Health San Antonio, San Antonio, TX, USADepartment of Urology, University of Texas Health San Antonio, San Antonio, TX, USADepartment of Urology, University of Texas Health San Antonio, San Antonio, TX, USADepartment of Cell and Molecular Biology, University of Texas Health San Antonio, San Antonio, TX, USADepartment of Urology, University of Texas Health San Antonio, San Antonio, TX, USADepartment of Urology, University of Texas Health San Antonio, San Antonio, TX, USAResphera Biosciences, Baltimore, MD, USADepartment of Urology, University of Texas Health San Antonio, San Antonio, TX, USAObjective. To perform a proof of concept microbiome evaluation and PD-L1 expression profiling in clear-cell renal cell carcinoma (cc-RCC) with associated tumor thrombus (TT). Methods. After IRB approval, six patients underwent radical nephrectomy (RN) with venous tumor thrombectomy (VTT). We collected fresh tissue specimens from normal adjacent, tumor, and thrombus tissues. We utilized RNA sequencing to obtain PD-L1 expression profiles and perform microbiome analysis. Statistical assessment was performed using Student’s t-test, chi-square, and spearman rank correlations using SPSS v25. Results. We noted the tumor thrombus to be mostly devoid of diverse microbiota. A large proportion of Staphylococcus epidermidus was detected and unknown if this is a surgical or postsurgical contaminant; however, it was noted more in the thrombus than other tissues. Microbiome diversity profiles were most abundant in the primary tumor compared to the thrombus or normal adjacent tissue. Differential expression of PD-L1 was examined in the tumor thrombus to the normal background tissue and noted three of the six subjects had a threshold above 2-fold. These three similar subjects had foreign microbiota that are typical residents of the oral microbiome. Conclusion. Renal tumors have more diverse microbiomes than normal adjacent tissue. Identification of resident oral microbiome profiles in clear-cell renal cancer with tumor thrombus provides a potential biomarker for thrombus response to PD-L1 inhibition.http://dx.doi.org/10.1155/2020/9068068 |
| spellingShingle | Michael A. Liss Yidong Chen Ronald Rodriguez Deepak Pruthi Teresa Johnson-Pais Hanzhang Wang Ahmed Mansour James R. White Dharam Kaushik Microbiome within Primary Tumor Tissue from Renal Cell Carcinoma May Be Associated with PD-L1 Expression of the Venous Tumor Thrombus Advances in Urology |
| title | Microbiome within Primary Tumor Tissue from Renal Cell Carcinoma May Be Associated with PD-L1 Expression of the Venous Tumor Thrombus |
| title_full | Microbiome within Primary Tumor Tissue from Renal Cell Carcinoma May Be Associated with PD-L1 Expression of the Venous Tumor Thrombus |
| title_fullStr | Microbiome within Primary Tumor Tissue from Renal Cell Carcinoma May Be Associated with PD-L1 Expression of the Venous Tumor Thrombus |
| title_full_unstemmed | Microbiome within Primary Tumor Tissue from Renal Cell Carcinoma May Be Associated with PD-L1 Expression of the Venous Tumor Thrombus |
| title_short | Microbiome within Primary Tumor Tissue from Renal Cell Carcinoma May Be Associated with PD-L1 Expression of the Venous Tumor Thrombus |
| title_sort | microbiome within primary tumor tissue from renal cell carcinoma may be associated with pd l1 expression of the venous tumor thrombus |
| url | http://dx.doi.org/10.1155/2020/9068068 |
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