Transcriptome and Metabolome Analyses of <i>Taxus chinensis</i> var. <i>mairei</i> Tissues Provide New Insights into the Regulation of Paclitaxel Biosynthesis
<i>Taxus</i> is the natural source of the anticancer drug paclitaxel. Although significant progress has been made in elucidating the biosynthetic pathway of paclitaxel, its tissue-specific accumulation and associated regulatory networks in <i>Taxus</i> remains unclear. In thi...
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2025-06-01
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| author | Luyuan Jiang Yanyan Li Xiaoyang Jiang Fenjuan Shao Wenli Wu Fan Xu Iain Wilson Angela Hoffman Yanfang Yang Deyou Qiu |
| author_facet | Luyuan Jiang Yanyan Li Xiaoyang Jiang Fenjuan Shao Wenli Wu Fan Xu Iain Wilson Angela Hoffman Yanfang Yang Deyou Qiu |
| author_sort | Luyuan Jiang |
| collection | DOAJ |
| description | <i>Taxus</i> is the natural source of the anticancer drug paclitaxel. Although significant progress has been made in elucidating the biosynthetic pathway of paclitaxel, its tissue-specific accumulation and associated regulatory networks in <i>Taxus</i> remains unclear. In this study, we conducted integrated transcriptomic and metabolomic analyses of the root, leaf, shoot, bark, and wood of <i>Taxus chinensis</i> var. <i>mairei</i> to investigate the tissue-specific biosynthesis and accumulation of paclitaxel. We found that paclitaxel, 10-deacetylbaccatin III, and several taxoids were significantly enriched in the leaf, bark, and shoot, while paclitaxel derivatives, such as taxayunnansin A and taxol B, accumulated primarily in the root. Most genes involved in paclitaxel biosynthesis showed the highest expression in the root and the lowest in the wood. Using weighted gene co-expression network analysis (WGCNA), we identified several candidate transcription factors potentially regulating paclitaxel biosynthesis. Further validation using yeast one-hybrid (Y1H) and dual-luciferase reporter assays confirmed that ERF68 activates the expression of <i>taxane-2α-hydroxylase</i> (T2H) gene, a key gene in the paclitaxel biosynthesis pathway. Collectively, our finding provides crucial insights into the transcriptional regulation of paclitaxel biosynthesis in <i>Taxus</i>. |
| format | Article |
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| institution | DOAJ |
| issn | 2223-7747 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
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| spelling | doaj-art-312079becf78496bba407ab2f7c971882025-08-20T03:16:36ZengMDPI AGPlants2223-77472025-06-011412177510.3390/plants14121775Transcriptome and Metabolome Analyses of <i>Taxus chinensis</i> var. <i>mairei</i> Tissues Provide New Insights into the Regulation of Paclitaxel BiosynthesisLuyuan Jiang0Yanyan Li1Xiaoyang Jiang2Fenjuan Shao3Wenli Wu4Fan Xu5Iain Wilson6Angela Hoffman7Yanfang Yang8Deyou Qiu9State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, ChinaCollege of Medicine, Pingdingshan University, Pingdingsha 467000, ChinaState Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, ChinaState Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, ChinaState Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, ChinaState Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, ChinaCSIRO Agriculture and Food, Canberra, ACT 2601, AustraliaDepartment of Natural Sciences, Saint Martin’s University, Lacey, WA 98503, USAState Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, ChinaState Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China<i>Taxus</i> is the natural source of the anticancer drug paclitaxel. Although significant progress has been made in elucidating the biosynthetic pathway of paclitaxel, its tissue-specific accumulation and associated regulatory networks in <i>Taxus</i> remains unclear. In this study, we conducted integrated transcriptomic and metabolomic analyses of the root, leaf, shoot, bark, and wood of <i>Taxus chinensis</i> var. <i>mairei</i> to investigate the tissue-specific biosynthesis and accumulation of paclitaxel. We found that paclitaxel, 10-deacetylbaccatin III, and several taxoids were significantly enriched in the leaf, bark, and shoot, while paclitaxel derivatives, such as taxayunnansin A and taxol B, accumulated primarily in the root. Most genes involved in paclitaxel biosynthesis showed the highest expression in the root and the lowest in the wood. Using weighted gene co-expression network analysis (WGCNA), we identified several candidate transcription factors potentially regulating paclitaxel biosynthesis. Further validation using yeast one-hybrid (Y1H) and dual-luciferase reporter assays confirmed that ERF68 activates the expression of <i>taxane-2α-hydroxylase</i> (T2H) gene, a key gene in the paclitaxel biosynthesis pathway. Collectively, our finding provides crucial insights into the transcriptional regulation of paclitaxel biosynthesis in <i>Taxus</i>.https://www.mdpi.com/2223-7747/14/12/1775<i>Taxus chinensis</i> var. <i>mairei</i>transcriptomemetabolomepaclitaxel biosynthesisregulation |
| spellingShingle | Luyuan Jiang Yanyan Li Xiaoyang Jiang Fenjuan Shao Wenli Wu Fan Xu Iain Wilson Angela Hoffman Yanfang Yang Deyou Qiu Transcriptome and Metabolome Analyses of <i>Taxus chinensis</i> var. <i>mairei</i> Tissues Provide New Insights into the Regulation of Paclitaxel Biosynthesis Plants <i>Taxus chinensis</i> var. <i>mairei</i> transcriptome metabolome paclitaxel biosynthesis regulation |
| title | Transcriptome and Metabolome Analyses of <i>Taxus chinensis</i> var. <i>mairei</i> Tissues Provide New Insights into the Regulation of Paclitaxel Biosynthesis |
| title_full | Transcriptome and Metabolome Analyses of <i>Taxus chinensis</i> var. <i>mairei</i> Tissues Provide New Insights into the Regulation of Paclitaxel Biosynthesis |
| title_fullStr | Transcriptome and Metabolome Analyses of <i>Taxus chinensis</i> var. <i>mairei</i> Tissues Provide New Insights into the Regulation of Paclitaxel Biosynthesis |
| title_full_unstemmed | Transcriptome and Metabolome Analyses of <i>Taxus chinensis</i> var. <i>mairei</i> Tissues Provide New Insights into the Regulation of Paclitaxel Biosynthesis |
| title_short | Transcriptome and Metabolome Analyses of <i>Taxus chinensis</i> var. <i>mairei</i> Tissues Provide New Insights into the Regulation of Paclitaxel Biosynthesis |
| title_sort | transcriptome and metabolome analyses of i taxus chinensis i var i mairei i tissues provide new insights into the regulation of paclitaxel biosynthesis |
| topic | <i>Taxus chinensis</i> var. <i>mairei</i> transcriptome metabolome paclitaxel biosynthesis regulation |
| url | https://www.mdpi.com/2223-7747/14/12/1775 |
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