Collagen Induced Arthritis in DBA/1J Mice Associates with Oxylipin Changes in Plasma
Oxylipins play important roles in various biological processes and are considered as mediators of inflammation for a wide range of diseases such as rheumatoid arthritis (RA). The purpose of this research was to study differences in oxylipin levels between a widely used collagen induced arthritis (CI...
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| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2015/543541 |
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| author | Min He Eduard van Wijk Ruud Berger Mei Wang Katrin Strassburg Johannes C. Schoeman Rob J. Vreeken Herman van Wietmarschen Amy C. Harms Masaki Kobayashi Thomas Hankemeier Jan van der Greef |
| author_facet | Min He Eduard van Wijk Ruud Berger Mei Wang Katrin Strassburg Johannes C. Schoeman Rob J. Vreeken Herman van Wietmarschen Amy C. Harms Masaki Kobayashi Thomas Hankemeier Jan van der Greef |
| author_sort | Min He |
| collection | DOAJ |
| description | Oxylipins play important roles in various biological processes and are considered as mediators of inflammation for a wide range of diseases such as rheumatoid arthritis (RA). The purpose of this research was to study differences in oxylipin levels between a widely used collagen induced arthritis (CIA) mice model and healthy control (Ctrl) mice. DBA/1J male mice (age: 6-7 weeks) were selected and randomly divided into two groups, namely, a CIA and a Ctrl group. The CIA mice were injected intraperitoneally (i.p.) with the joint cartilage component collagen type II (CII) and an adjuvant injection of lipopolysaccharide (LPS). Oxylipin metabolites were extracted from plasma for each individual sample using solid phase extraction (SPE) and were detected with high performance liquid chromatography/tandem mass spectrometry (HPLC-ESI-MS/MS), using dynamic multiple reaction monitoring (dMRM). Both univariate and multivariate statistical analyses were applied. The results in univariate Student’s t-test revealed 10 significantly up- or downregulated oxylipins in CIA mice, which were supplemented by another 6 additional oxylipins, contributing to group clustering upon multivariate analysis. The dysregulation of these oxylipins revealed the presence of ROS-generated oxylipins and an increase of inflammation in CIA mice. The results also suggested that the collagen induced arthritis might associate with dysregulation of apoptosis, possibly inhibited by activated NF-κB because of insufficient PPAR-γ ligands. |
| format | Article |
| id | doaj-art-311e2a59b868497abbc136e4cbc55871 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-311e2a59b868497abbc136e4cbc558712025-02-03T05:46:22ZengWileyMediators of Inflammation0962-93511466-18612015-01-01201510.1155/2015/543541543541Collagen Induced Arthritis in DBA/1J Mice Associates with Oxylipin Changes in PlasmaMin He0Eduard van Wijk1Ruud Berger2Mei Wang3Katrin Strassburg4Johannes C. Schoeman5Rob J. Vreeken6Herman van Wietmarschen7Amy C. Harms8Masaki Kobayashi9Thomas Hankemeier10Jan van der Greef11Analytical BioSciences, LACDR, Leiden University, P.O. Box 9502, 2300 RA Leiden, NetherlandsAnalytical BioSciences, LACDR, Leiden University, P.O. Box 9502, 2300 RA Leiden, NetherlandsAnalytical BioSciences, LACDR, Leiden University, P.O. Box 9502, 2300 RA Leiden, NetherlandsAnalytical BioSciences, LACDR, Leiden University, P.O. Box 9502, 2300 RA Leiden, NetherlandsAnalytical BioSciences, LACDR, Leiden University, P.O. Box 9502, 2300 RA Leiden, NetherlandsAnalytical BioSciences, LACDR, Leiden University, P.O. Box 9502, 2300 RA Leiden, NetherlandsAnalytical BioSciences, LACDR, Leiden University, P.O. Box 9502, 2300 RA Leiden, NetherlandsAnalytical BioSciences, LACDR, Leiden University, P.O. Box 9502, 2300 RA Leiden, NetherlandsAnalytical BioSciences, LACDR, Leiden University, P.O. Box 9502, 2300 RA Leiden, NetherlandsTohoku Institute of Technology, Sendai 982-8577, JapanAnalytical BioSciences, LACDR, Leiden University, P.O. Box 9502, 2300 RA Leiden, NetherlandsAnalytical BioSciences, LACDR, Leiden University, P.O. Box 9502, 2300 RA Leiden, NetherlandsOxylipins play important roles in various biological processes and are considered as mediators of inflammation for a wide range of diseases such as rheumatoid arthritis (RA). The purpose of this research was to study differences in oxylipin levels between a widely used collagen induced arthritis (CIA) mice model and healthy control (Ctrl) mice. DBA/1J male mice (age: 6-7 weeks) were selected and randomly divided into two groups, namely, a CIA and a Ctrl group. The CIA mice were injected intraperitoneally (i.p.) with the joint cartilage component collagen type II (CII) and an adjuvant injection of lipopolysaccharide (LPS). Oxylipin metabolites were extracted from plasma for each individual sample using solid phase extraction (SPE) and were detected with high performance liquid chromatography/tandem mass spectrometry (HPLC-ESI-MS/MS), using dynamic multiple reaction monitoring (dMRM). Both univariate and multivariate statistical analyses were applied. The results in univariate Student’s t-test revealed 10 significantly up- or downregulated oxylipins in CIA mice, which were supplemented by another 6 additional oxylipins, contributing to group clustering upon multivariate analysis. The dysregulation of these oxylipins revealed the presence of ROS-generated oxylipins and an increase of inflammation in CIA mice. The results also suggested that the collagen induced arthritis might associate with dysregulation of apoptosis, possibly inhibited by activated NF-κB because of insufficient PPAR-γ ligands.http://dx.doi.org/10.1155/2015/543541 |
| spellingShingle | Min He Eduard van Wijk Ruud Berger Mei Wang Katrin Strassburg Johannes C. Schoeman Rob J. Vreeken Herman van Wietmarschen Amy C. Harms Masaki Kobayashi Thomas Hankemeier Jan van der Greef Collagen Induced Arthritis in DBA/1J Mice Associates with Oxylipin Changes in Plasma Mediators of Inflammation |
| title | Collagen Induced Arthritis in DBA/1J Mice Associates with Oxylipin Changes in Plasma |
| title_full | Collagen Induced Arthritis in DBA/1J Mice Associates with Oxylipin Changes in Plasma |
| title_fullStr | Collagen Induced Arthritis in DBA/1J Mice Associates with Oxylipin Changes in Plasma |
| title_full_unstemmed | Collagen Induced Arthritis in DBA/1J Mice Associates with Oxylipin Changes in Plasma |
| title_short | Collagen Induced Arthritis in DBA/1J Mice Associates with Oxylipin Changes in Plasma |
| title_sort | collagen induced arthritis in dba 1j mice associates with oxylipin changes in plasma |
| url | http://dx.doi.org/10.1155/2015/543541 |
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