Utilization of Hydrolysis of Protein Concentrates Prepared From the Waste of Common Carp Fish using Trypsin Enzyme for the Growth of Lactic Acid Bacteria
This study aimed to use trypsin enzyme hydrolysates of protein concentrates prepared from the waste of common carp fish (Cyprinus carpio) for the growth of lactic acid bacteria. Trypsin enzyme has been used in the hydrolysis of acid and base protein concentrates for various times, including 60, 120,...
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University of Diyala
2024-12-01
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| Series: | مجلة ديالى للعلوم الزراعية |
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| Online Access: | https://journal.djas.uodiyala.edu.iq/index.php/dasj/article/view/9035 |
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| author | Ammar Shehab Mohammed Elham Isnael AL-Shamary |
| author_facet | Ammar Shehab Mohammed Elham Isnael AL-Shamary |
| author_sort | Ammar Shehab Mohammed |
| collection | DOAJ |
| description | This study aimed to use trypsin enzyme hydrolysates of protein concentrates prepared from the waste of common carp fish (Cyprinus carpio) for the growth of lactic acid bacteria. Trypsin enzyme has been used in the hydrolysis of acid and base protein concentrates for various times, including 60, 120, and 180 minutes. The solubility and emulsification property of the produced hydrolysates was studied. The EAI and ESI at pH 2 and 12 gave the highest values for solubility, , while the lowest values at pH 4. Hydrolysates were used to prepare culture media as an alternative to the MRS medium. Peptospecial and Beef extract were replaced in the commercial media with hydrolysates, and two types of culture media were prepared, one containing yeast extract and the other without it. The enzymatic reaction time had a significant effect on the number of Lactobacillus acidophilus and Bifidobacterium animalis colonies, and yeast extract had a significant effect on the number of colonies of Lactobacillus rhamnosus GG. In contrast, the enzymatic reaction time had not affected the number of colonies of Lactobacillus plantarum and Bifidobacterium infantis. This study is recommended to study the response of other types of lactic acid bacteria and the suitability of the culture media prepared in this study to their growth requirements. |
| format | Article |
| id | doaj-art-310fc72ca9b44dffbe8a4dddb5548c0d |
| institution | Kabale University |
| issn | 2073-9524 2310-8746 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | University of Diyala |
| record_format | Article |
| series | مجلة ديالى للعلوم الزراعية |
| spelling | doaj-art-310fc72ca9b44dffbe8a4dddb5548c0d2025-01-05T09:03:49ZengUniversity of Diyalaمجلة ديالى للعلوم الزراعية2073-95242310-87462024-12-01162122610.52951/dasj.24160202Utilization of Hydrolysis of Protein Concentrates Prepared From the Waste of Common Carp Fish using Trypsin Enzyme for the Growth of Lactic Acid BacteriaAmmar Shehab Mohammed0https://orcid.org/0009-0008-3778-6111Elham Isnael AL-Shamary1https://orcid.org/0000-0002-1354-3202Department of Food Sciences, College of Agricultural Engineering Sciences, University of Baghdad, Baghdad, Iraq.Department of Food Sciences, College of Agricultural Engineering Sciences, University of Baghdad, Baghdad, Iraq.This study aimed to use trypsin enzyme hydrolysates of protein concentrates prepared from the waste of common carp fish (Cyprinus carpio) for the growth of lactic acid bacteria. Trypsin enzyme has been used in the hydrolysis of acid and base protein concentrates for various times, including 60, 120, and 180 minutes. The solubility and emulsification property of the produced hydrolysates was studied. The EAI and ESI at pH 2 and 12 gave the highest values for solubility, , while the lowest values at pH 4. Hydrolysates were used to prepare culture media as an alternative to the MRS medium. Peptospecial and Beef extract were replaced in the commercial media with hydrolysates, and two types of culture media were prepared, one containing yeast extract and the other without it. The enzymatic reaction time had a significant effect on the number of Lactobacillus acidophilus and Bifidobacterium animalis colonies, and yeast extract had a significant effect on the number of colonies of Lactobacillus rhamnosus GG. In contrast, the enzymatic reaction time had not affected the number of colonies of Lactobacillus plantarum and Bifidobacterium infantis. This study is recommended to study the response of other types of lactic acid bacteria and the suitability of the culture media prepared in this study to their growth requirements.https://journal.djas.uodiyala.edu.iq/index.php/dasj/article/view/9035carp fish wasteemulsificationlactic acid bacteriasolubility |
| spellingShingle | Ammar Shehab Mohammed Elham Isnael AL-Shamary Utilization of Hydrolysis of Protein Concentrates Prepared From the Waste of Common Carp Fish using Trypsin Enzyme for the Growth of Lactic Acid Bacteria مجلة ديالى للعلوم الزراعية carp fish waste emulsification lactic acid bacteria solubility |
| title | Utilization of Hydrolysis of Protein Concentrates Prepared From the Waste of Common Carp Fish using Trypsin Enzyme for the Growth of Lactic Acid Bacteria |
| title_full | Utilization of Hydrolysis of Protein Concentrates Prepared From the Waste of Common Carp Fish using Trypsin Enzyme for the Growth of Lactic Acid Bacteria |
| title_fullStr | Utilization of Hydrolysis of Protein Concentrates Prepared From the Waste of Common Carp Fish using Trypsin Enzyme for the Growth of Lactic Acid Bacteria |
| title_full_unstemmed | Utilization of Hydrolysis of Protein Concentrates Prepared From the Waste of Common Carp Fish using Trypsin Enzyme for the Growth of Lactic Acid Bacteria |
| title_short | Utilization of Hydrolysis of Protein Concentrates Prepared From the Waste of Common Carp Fish using Trypsin Enzyme for the Growth of Lactic Acid Bacteria |
| title_sort | utilization of hydrolysis of protein concentrates prepared from the waste of common carp fish using trypsin enzyme for the growth of lactic acid bacteria |
| topic | carp fish waste emulsification lactic acid bacteria solubility |
| url | https://journal.djas.uodiyala.edu.iq/index.php/dasj/article/view/9035 |
| work_keys_str_mv | AT ammarshehabmohammed utilizationofhydrolysisofproteinconcentratespreparedfromthewasteofcommoncarpfishusingtrypsinenzymeforthegrowthoflacticacidbacteria AT elhamisnaelalshamary utilizationofhydrolysisofproteinconcentratespreparedfromthewasteofcommoncarpfishusingtrypsinenzymeforthegrowthoflacticacidbacteria |