Effects of Sildenafil on Cognitive Function Recovery and Neuronal Cell Death Protection after Transient Global Cerebral Ischemia in Gerbils
Cerebral ischemic stroke is a major cause of death worldwide due to brain cell death resulting from ischemia-reperfusion injury. However, effective treatment approaches for patients with ischemic stroke are still lacking in clinical practice. This study investigated the potential neuroprotective eff...
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MDPI AG
2024-09-01
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| author | Yeon Hee Yu Gun Woo Kim Yu Ran Lee Dae-Kyoon Park Beomjong Song Duk-Soo Kim |
| author_facet | Yeon Hee Yu Gun Woo Kim Yu Ran Lee Dae-Kyoon Park Beomjong Song Duk-Soo Kim |
| author_sort | Yeon Hee Yu |
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| description | Cerebral ischemic stroke is a major cause of death worldwide due to brain cell death resulting from ischemia-reperfusion injury. However, effective treatment approaches for patients with ischemic stroke are still lacking in clinical practice. This study investigated the potential neuroprotective effects of sildenafil, a phosphodiesterase-5 inhibitor, in a gerbil model of global brain ischemia. We investigated the effects of sildenafil on the expression of glial fibrillary acidic protein and aquaporin-4, which are markers related to astrocyte activation and water homeostasis, respectively. Immunofluorescence analysis showed that the number of cells co-expressing these markers, which was elevated in the ischemia-induced group, was significantly reduced in the sildenafil-treated groups. This suggests that sildenafil may have a potential mitigating effect on astrocyte activation induced by ischemia. Additionally, we performed various behavioral tests, including the open-field test, novel object recognition, Barnes maze, Y-maze, and passive avoidance tests, to evaluate sildenafil’s effect on cognitive function impaired by ischemia. Overall, the results suggest that sildenafil may serve as a neuroprotective agent, potentially alleviating delayed neuronal cell death and improving cognitive function impaired by ischemia. |
| format | Article |
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| institution | OA Journals |
| issn | 2227-9059 |
| language | English |
| publishDate | 2024-09-01 |
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| series | Biomedicines |
| spelling | doaj-art-30ffadd9e4254cbb9f69534dd3cc9e262025-08-20T01:56:10ZengMDPI AGBiomedicines2227-90592024-09-01129207710.3390/biomedicines12092077Effects of Sildenafil on Cognitive Function Recovery and Neuronal Cell Death Protection after Transient Global Cerebral Ischemia in GerbilsYeon Hee Yu0Gun Woo Kim1Yu Ran Lee2Dae-Kyoon Park3Beomjong Song4Duk-Soo Kim5Department of Anatomy, College of Medicine, Soonchunhyang University, Cheonan 31151, Republic of KoreaResearch Supporting Center for Medical Science, College of Medicine, Dong-A, Busan 49201, Republic of KoreaDepartment of Anatomy, College of Medicine, Soonchunhyang University, Cheonan 31151, Republic of KoreaDepartment of Anatomy, College of Medicine, Soonchunhyang University, Cheonan 31151, Republic of KoreaDepartment of Anatomy, College of Medicine, Soonchunhyang University, Cheonan 31151, Republic of KoreaDepartment of Anatomy, College of Medicine, Soonchunhyang University, Cheonan 31151, Republic of KoreaCerebral ischemic stroke is a major cause of death worldwide due to brain cell death resulting from ischemia-reperfusion injury. However, effective treatment approaches for patients with ischemic stroke are still lacking in clinical practice. This study investigated the potential neuroprotective effects of sildenafil, a phosphodiesterase-5 inhibitor, in a gerbil model of global brain ischemia. We investigated the effects of sildenafil on the expression of glial fibrillary acidic protein and aquaporin-4, which are markers related to astrocyte activation and water homeostasis, respectively. Immunofluorescence analysis showed that the number of cells co-expressing these markers, which was elevated in the ischemia-induced group, was significantly reduced in the sildenafil-treated groups. This suggests that sildenafil may have a potential mitigating effect on astrocyte activation induced by ischemia. Additionally, we performed various behavioral tests, including the open-field test, novel object recognition, Barnes maze, Y-maze, and passive avoidance tests, to evaluate sildenafil’s effect on cognitive function impaired by ischemia. Overall, the results suggest that sildenafil may serve as a neuroprotective agent, potentially alleviating delayed neuronal cell death and improving cognitive function impaired by ischemia.https://www.mdpi.com/2227-9059/12/9/2077global ischemiareperfusionsildenafilbehavioral testneuroprotection |
| spellingShingle | Yeon Hee Yu Gun Woo Kim Yu Ran Lee Dae-Kyoon Park Beomjong Song Duk-Soo Kim Effects of Sildenafil on Cognitive Function Recovery and Neuronal Cell Death Protection after Transient Global Cerebral Ischemia in Gerbils Biomedicines global ischemia reperfusion sildenafil behavioral test neuroprotection |
| title | Effects of Sildenafil on Cognitive Function Recovery and Neuronal Cell Death Protection after Transient Global Cerebral Ischemia in Gerbils |
| title_full | Effects of Sildenafil on Cognitive Function Recovery and Neuronal Cell Death Protection after Transient Global Cerebral Ischemia in Gerbils |
| title_fullStr | Effects of Sildenafil on Cognitive Function Recovery and Neuronal Cell Death Protection after Transient Global Cerebral Ischemia in Gerbils |
| title_full_unstemmed | Effects of Sildenafil on Cognitive Function Recovery and Neuronal Cell Death Protection after Transient Global Cerebral Ischemia in Gerbils |
| title_short | Effects of Sildenafil on Cognitive Function Recovery and Neuronal Cell Death Protection after Transient Global Cerebral Ischemia in Gerbils |
| title_sort | effects of sildenafil on cognitive function recovery and neuronal cell death protection after transient global cerebral ischemia in gerbils |
| topic | global ischemia reperfusion sildenafil behavioral test neuroprotection |
| url | https://www.mdpi.com/2227-9059/12/9/2077 |
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