Essential Microenvironment for Thymopoiesis is Preserved in Human Adult and Aged Thymus
Normal human thymuses at various ages were immunohistologically examined in order to determine whether adult or aged thymus maintained the microenvironment for the T cell development and thymopoiesis was really ongoing. To analyze the thymic microenvironment, two monoclonal antibodies (MoAb) were em...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2003-01-01
|
| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1080/10446670310001598465 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832562103217553408 |
|---|---|
| author | J. Shiraishi M. Utsuyama S. Seki H. Akamatsu M. Sunamori M. Kasai K. Hirokawa |
| author_facet | J. Shiraishi M. Utsuyama S. Seki H. Akamatsu M. Sunamori M. Kasai K. Hirokawa |
| author_sort | J. Shiraishi |
| collection | DOAJ |
| description | Normal human thymuses at various ages were immunohistologically examined in order to determine whether adult or aged thymus maintained the microenvironment for the T cell development and thymopoiesis was really ongoing. To analyze the thymic microenvironment, two monoclonal antibodies (MoAb) were employed. One is MoAb to IL-1 receptor (IL-1R) recognizing medullary and subcapsular cortical epithelial cells of normal infant human thymus. The other is UH-1 MoAb recognizing thymic epithelial cells within the cortex, which are negative with IL-1R-MoAb. Thymus of subjects over 20 years of age was split into many fragments and dispersed in the fatty tissue. However, the microenvironment of each fragment was composed of both IL-1R positive and UH-1 positive epithelial cells, and the UH-1 positive portion was populated with lymphocytes showing a follicle-like appearance. Lymphocytes in these follicle-like portions were mostly CD4+CD8+ double positive cells and contained many proliferating cells as well as apoptotic cells. Thus these follicle-like portions in adult and aged thymus were considered to be functioning as cortex as in infant thymus. Proliferative activity of thymocytes in the thymic cortex and the follicle-like portions definitely declined with advance of age, while incidence of apoptotic thymocytes increased with aging. |
| format | Article |
| id | doaj-art-30f60c2414614381b279935d76479aba |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2003-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-30f60c2414614381b279935d76479aba2025-02-03T01:23:27ZengWileyClinical and Developmental Immunology1740-25221740-25302003-01-01101535910.1080/10446670310001598465Essential Microenvironment for Thymopoiesis is Preserved in Human Adult and Aged ThymusJ. Shiraishi0M. Utsuyama1S. Seki2H. Akamatsu3M. Sunamori4M. Kasai5K. Hirokawa6Department of Pathology and Immunology, Tokyo Medical and Dental University Graduate School, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8519, JapanDepartment of Pathology and Immunology, Tokyo Medical and Dental University Graduate School, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8519, JapanDepartment of Pathology and Immunology, Tokyo Medical and Dental University Graduate School, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8519, JapanDepartment of Cardiothoracic Surgery, Tokyo Medical and Dental University Graduate School, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8519, JapanDepartment of Cardiothoracic Surgery, Tokyo Medical and Dental University Graduate School, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8519, JapanDepartment of Bacterial and Blood Products, National Institute of Infectious Diseases, 1-23-1, Toyama, Shinjuku-ku, Tokyo 162-8640, JapanDepartment of Pathology and Immunology, Tokyo Medical and Dental University Graduate School, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8519, JapanNormal human thymuses at various ages were immunohistologically examined in order to determine whether adult or aged thymus maintained the microenvironment for the T cell development and thymopoiesis was really ongoing. To analyze the thymic microenvironment, two monoclonal antibodies (MoAb) were employed. One is MoAb to IL-1 receptor (IL-1R) recognizing medullary and subcapsular cortical epithelial cells of normal infant human thymus. The other is UH-1 MoAb recognizing thymic epithelial cells within the cortex, which are negative with IL-1R-MoAb. Thymus of subjects over 20 years of age was split into many fragments and dispersed in the fatty tissue. However, the microenvironment of each fragment was composed of both IL-1R positive and UH-1 positive epithelial cells, and the UH-1 positive portion was populated with lymphocytes showing a follicle-like appearance. Lymphocytes in these follicle-like portions were mostly CD4+CD8+ double positive cells and contained many proliferating cells as well as apoptotic cells. Thus these follicle-like portions in adult and aged thymus were considered to be functioning as cortex as in infant thymus. Proliferative activity of thymocytes in the thymic cortex and the follicle-like portions definitely declined with advance of age, while incidence of apoptotic thymocytes increased with aging.http://dx.doi.org/10.1080/10446670310001598465 |
| spellingShingle | J. Shiraishi M. Utsuyama S. Seki H. Akamatsu M. Sunamori M. Kasai K. Hirokawa Essential Microenvironment for Thymopoiesis is Preserved in Human Adult and Aged Thymus Clinical and Developmental Immunology |
| title | Essential Microenvironment for Thymopoiesis is Preserved in Human Adult and Aged Thymus |
| title_full | Essential Microenvironment for Thymopoiesis is Preserved in Human Adult and Aged Thymus |
| title_fullStr | Essential Microenvironment for Thymopoiesis is Preserved in Human Adult and Aged Thymus |
| title_full_unstemmed | Essential Microenvironment for Thymopoiesis is Preserved in Human Adult and Aged Thymus |
| title_short | Essential Microenvironment for Thymopoiesis is Preserved in Human Adult and Aged Thymus |
| title_sort | essential microenvironment for thymopoiesis is preserved in human adult and aged thymus |
| url | http://dx.doi.org/10.1080/10446670310001598465 |
| work_keys_str_mv | AT jshiraishi essentialmicroenvironmentforthymopoiesisispreservedinhumanadultandagedthymus AT mutsuyama essentialmicroenvironmentforthymopoiesisispreservedinhumanadultandagedthymus AT sseki essentialmicroenvironmentforthymopoiesisispreservedinhumanadultandagedthymus AT hakamatsu essentialmicroenvironmentforthymopoiesisispreservedinhumanadultandagedthymus AT msunamori essentialmicroenvironmentforthymopoiesisispreservedinhumanadultandagedthymus AT mkasai essentialmicroenvironmentforthymopoiesisispreservedinhumanadultandagedthymus AT khirokawa essentialmicroenvironmentforthymopoiesisispreservedinhumanadultandagedthymus |