Affinity selection mass spectrometry (AS-MS) for prospecting ligands in natural product libraries
Natural product libraries are a source of diverse 3D molecular features furnishing an array of biological functions in drug discovery research. In this paper we will oversee the use of affinity selection mass spectrometry (AS-MS) for identifying ligands to a variety of biological targets using natur...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-05-01
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| Series: | Frontiers in Natural Products |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fntpr.2025.1562501/full |
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| author | Bruno S. do Amaral Marcela C. de Moraes Carmen L. Cardoso Quezia B. Cass |
| author_facet | Bruno S. do Amaral Marcela C. de Moraes Carmen L. Cardoso Quezia B. Cass |
| author_sort | Bruno S. do Amaral |
| collection | DOAJ |
| description | Natural product libraries are a source of diverse 3D molecular features furnishing an array of biological functions in drug discovery research. In this paper we will oversee the use of affinity selection mass spectrometry (AS-MS) for identifying ligands to a variety of biological targets using natural products as the molecular library. The assay modes used in solution or with the immobilized target and their pros and cons will be presented. Additionally, the required experiments and investigation for accurate chemical annotation of the disclosed ligands in a non-target assay are discussed. |
| format | Article |
| id | doaj-art-30dce4ba25f84f46b2cd18b39f52f0e4 |
| institution | Kabale University |
| issn | 2813-2602 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Natural Products |
| spelling | doaj-art-30dce4ba25f84f46b2cd18b39f52f0e42025-08-20T03:28:48ZengFrontiers Media S.A.Frontiers in Natural Products2813-26022025-05-01410.3389/fntpr.2025.15625011562501Affinity selection mass spectrometry (AS-MS) for prospecting ligands in natural product librariesBruno S. do Amaral0Marcela C. de Moraes1Carmen L. Cardoso2Quezia B. Cass3Instituto Federal de Educação, Ciência e Tecnologia de São Paulo, São Paulo, BrazilDepartamento de Química Orgânica, BioCrom – Laboratório de Cromatografia de Bioafinidade e Química Ambiental, Instituto de Química, Universidade Federal Fluminense, Niterói, BrazilDepartamento de Química, Grupo de Cromatografia de Bioafinidade e Produtos Naturais (GCBPN), Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, BrazilSeparare, Departamento de Química, Universidade Federal de São Carlos, São Carlos, BrazilNatural product libraries are a source of diverse 3D molecular features furnishing an array of biological functions in drug discovery research. In this paper we will oversee the use of affinity selection mass spectrometry (AS-MS) for identifying ligands to a variety of biological targets using natural products as the molecular library. The assay modes used in solution or with the immobilized target and their pros and cons will be presented. Additionally, the required experiments and investigation for accurate chemical annotation of the disclosed ligands in a non-target assay are discussed.https://www.frontiersin.org/articles/10.3389/fntpr.2025.1562501/fullligand discoveryimmobilized targetssolution-based assayschemical annotationnon-functional assaysmolecular library |
| spellingShingle | Bruno S. do Amaral Marcela C. de Moraes Carmen L. Cardoso Quezia B. Cass Affinity selection mass spectrometry (AS-MS) for prospecting ligands in natural product libraries Frontiers in Natural Products ligand discovery immobilized targets solution-based assays chemical annotation non-functional assays molecular library |
| title | Affinity selection mass spectrometry (AS-MS) for prospecting ligands in natural product libraries |
| title_full | Affinity selection mass spectrometry (AS-MS) for prospecting ligands in natural product libraries |
| title_fullStr | Affinity selection mass spectrometry (AS-MS) for prospecting ligands in natural product libraries |
| title_full_unstemmed | Affinity selection mass spectrometry (AS-MS) for prospecting ligands in natural product libraries |
| title_short | Affinity selection mass spectrometry (AS-MS) for prospecting ligands in natural product libraries |
| title_sort | affinity selection mass spectrometry as ms for prospecting ligands in natural product libraries |
| topic | ligand discovery immobilized targets solution-based assays chemical annotation non-functional assays molecular library |
| url | https://www.frontiersin.org/articles/10.3389/fntpr.2025.1562501/full |
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