Perspectives of RNAi, CUADb and CRISPR/Cas as Innovative Antisense Technologies for Insect Pest Control: From Discovery to Practice
Pest management is undergoing a transformative shift with the development of the cutting-edge antisense technologies: RNA interference (RNAi), contact unmodified antisense DNA biotechnology (CUADb), and the CRISPR-associated proteins (CRISPR/Cas). These approaches function by facilitating sequence-s...
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MDPI AG
2025-07-01
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| Series: | Insects |
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| author | Hemant Kumar Nikita Gal’chinsky Verma Sweta Nikita Negi Roman Filatov Anamika Chandel Jamin Ali Vol Oberemok Kate Laikova |
| author_facet | Hemant Kumar Nikita Gal’chinsky Verma Sweta Nikita Negi Roman Filatov Anamika Chandel Jamin Ali Vol Oberemok Kate Laikova |
| author_sort | Hemant Kumar |
| collection | DOAJ |
| description | Pest management is undergoing a transformative shift with the development of the cutting-edge antisense technologies: RNA interference (RNAi), contact unmodified antisense DNA biotechnology (CUADb), and the CRISPR-associated proteins (CRISPR/Cas). These approaches function by facilitating sequence-specific pairing of nucleic acids followed by nuclease-mediated cleavage, offering exceptional precision for targeted pest control. While RNA-guided mechanisms such as RNAi and CRISPR/Cas were initially characterized in non-insect systems, primarily as innate defenses against viral infections, the DNA-guided CUADb pathway was first identified in insect pests as a functional pest control strategy. Its broader role in ribosomal RNA (rRNA) biogenesis was recognized later. Together, these discoveries have revealed an entirely new dimension of gene regulation, with profound implications for sustainable pest management. Despite sharing a common principle of sequence-specific targeting RNAi, CUADb, and CRISPR/Cas differ in several key aspects, including their mechanisms of action, target specificity, and applicability. Rather than serving as universal solutions, each technology is likely to be optimally effective against specific pest groups. Moreover, these technologies allow for rapid adaptation of control strategies to overcome target-site resistance, ensuring long-term efficacy. This review summarizes the core functional characteristics, potential applications, and current limitations of each antisense technology, emphasizing their complementary roles in advancing environmentally sustainable pest control. By integrating foundational biological discoveries with applied innovations, this work provides a new perspectives on incorporating antisense-based strategies into next-generation integrated pest management systems. |
| format | Article |
| id | doaj-art-30d8314665524897a444deebb4a8a3b1 |
| institution | DOAJ |
| issn | 2075-4450 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
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| series | Insects |
| spelling | doaj-art-30d8314665524897a444deebb4a8a3b12025-08-20T03:08:09ZengMDPI AGInsects2075-44502025-07-0116774610.3390/insects16070746Perspectives of RNAi, CUADb and CRISPR/Cas as Innovative Antisense Technologies for Insect Pest Control: From Discovery to PracticeHemant Kumar0Nikita Gal’chinsky1Verma Sweta2Nikita Negi3Roman Filatov4Anamika Chandel5Jamin Ali6Vol Oberemok7Kate Laikova8Department of Entomology, Agriculture College, Garhwa, Birsa Agricultural University, Ranchi 834006, IndiaDepartment of General Biology and Genetics, V.I. Vernadsky Crimean Federal University, 295007 Simferopol, RussiaDivision of Entomology, ICAR-Indian Agricultural Research Institute, New Delhi 110012, IndiaDivision of Genomic Resources, National Bureau of Agricultural Insect Resources, Bengaluru 560024, IndiaDepartment of General Biology and Genetics, V.I. Vernadsky Crimean Federal University, 295007 Simferopol, RussiaDivision of Vegetable Sciences, ICAR-Indian Agricultural Research Institute, New Delhi 110012, IndiaCollege of Plant Protection, Jilin Agricultural University, Changchun 130118, ChinaDepartment of General Biology and Genetics, V.I. Vernadsky Crimean Federal University, 295007 Simferopol, RussiaDepartment of General Biology and Genetics, V.I. Vernadsky Crimean Federal University, 295007 Simferopol, RussiaPest management is undergoing a transformative shift with the development of the cutting-edge antisense technologies: RNA interference (RNAi), contact unmodified antisense DNA biotechnology (CUADb), and the CRISPR-associated proteins (CRISPR/Cas). These approaches function by facilitating sequence-specific pairing of nucleic acids followed by nuclease-mediated cleavage, offering exceptional precision for targeted pest control. While RNA-guided mechanisms such as RNAi and CRISPR/Cas were initially characterized in non-insect systems, primarily as innate defenses against viral infections, the DNA-guided CUADb pathway was first identified in insect pests as a functional pest control strategy. Its broader role in ribosomal RNA (rRNA) biogenesis was recognized later. Together, these discoveries have revealed an entirely new dimension of gene regulation, with profound implications for sustainable pest management. Despite sharing a common principle of sequence-specific targeting RNAi, CUADb, and CRISPR/Cas differ in several key aspects, including their mechanisms of action, target specificity, and applicability. Rather than serving as universal solutions, each technology is likely to be optimally effective against specific pest groups. Moreover, these technologies allow for rapid adaptation of control strategies to overcome target-site resistance, ensuring long-term efficacy. This review summarizes the core functional characteristics, potential applications, and current limitations of each antisense technology, emphasizing their complementary roles in advancing environmentally sustainable pest control. By integrating foundational biological discoveries with applied innovations, this work provides a new perspectives on incorporating antisense-based strategies into next-generation integrated pest management systems.https://www.mdpi.com/2075-4450/16/7/746RNAiCUADbCRISPR/Casantisense technologiesinsect pest control |
| spellingShingle | Hemant Kumar Nikita Gal’chinsky Verma Sweta Nikita Negi Roman Filatov Anamika Chandel Jamin Ali Vol Oberemok Kate Laikova Perspectives of RNAi, CUADb and CRISPR/Cas as Innovative Antisense Technologies for Insect Pest Control: From Discovery to Practice Insects RNAi CUADb CRISPR/Cas antisense technologies insect pest control |
| title | Perspectives of RNAi, CUADb and CRISPR/Cas as Innovative Antisense Technologies for Insect Pest Control: From Discovery to Practice |
| title_full | Perspectives of RNAi, CUADb and CRISPR/Cas as Innovative Antisense Technologies for Insect Pest Control: From Discovery to Practice |
| title_fullStr | Perspectives of RNAi, CUADb and CRISPR/Cas as Innovative Antisense Technologies for Insect Pest Control: From Discovery to Practice |
| title_full_unstemmed | Perspectives of RNAi, CUADb and CRISPR/Cas as Innovative Antisense Technologies for Insect Pest Control: From Discovery to Practice |
| title_short | Perspectives of RNAi, CUADb and CRISPR/Cas as Innovative Antisense Technologies for Insect Pest Control: From Discovery to Practice |
| title_sort | perspectives of rnai cuadb and crispr cas as innovative antisense technologies for insect pest control from discovery to practice |
| topic | RNAi CUADb CRISPR/Cas antisense technologies insect pest control |
| url | https://www.mdpi.com/2075-4450/16/7/746 |
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